Results 101 to 110 of about 3,017,680 (323)
Circular dichroism and conformational polymorphism of DNA [PDF]
Nucleic Acids Research, 2009 Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures.
Jaroslav Kypr +3 more
openaire +3 more sources
Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]
, 1993 The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini +3 more
core +1 more source
CSA: An efficient algorithm to improve circular DNA multiple alignment
BMC Bioinformatics, 2009 Background The comparison of homologous sequences from different species is an essential approach to reconstruct the evolutionary history of species and of the genes they harbour in their genomes.
Pereira Luísa +2 more
doaj +1 more source
Circulating histones as clinical biomarkers in critically ill conditions
FEBS Letters, EarlyView.Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez +17 more
wiley +1 more source
AIP Advances, 2015 The equilibrium configuration equations of DNA chain with elastic rod model are derived in detail by the variation of the free energy functional, which depends on the curvature, torsion, twisting angle and its derivative with respect to the arc-length of
Ye Xiao, Zaixing Huang
doaj +1 more source
TempliPhi, ⌽29 DNA Polymerase Based Rolling Circle Amplification of Templates for DNA Sequencing
BioTechniques, 2002 We have developed a novel, isothermal DNA amplification strategy that employs ⌽29 DNA polymerase and rolling circle amplification to generate high-quality templates for DNA sequencing reactions. The TempliPhi™ DNA amplification kits take advantage of the
John R. Nelson +10 more
doaj +1 more source
Similar gene estimates from circular and linear standards in quantitative PCR analyses using the prokaryotic 16S rRNA gene as a model. [PDF]
PLoS ONE, 2012 Quantitative PCR (qPCR) is one of the most widely used tools for quantifying absolute numbers of microbial gene copies in test samples. A recent publication showed that circular plasmid DNA standards grossly overestimated numbers of a target gene by as ...
Athenia L Oldham, Kathleen E Duncan
doaj +1 more source
FEBS Letters, EarlyView.Exposure to common noxious agents (1), including allergens, pollutants, and micro‐nanoplastics, can cause epithelial barrier damage (2) in our body's protective linings. This may trigger an immune response to our microbiome (3). The epithelial barrier theory explains how this process can lead to chronic noncommunicable diseases (4) affecting organs ...
Can Zeyneloglu +17 more
wiley +1 more source
Entropic penalties in circular DNA assembly [PDF]
The Journal of Chemical Physics, 2014 The thermodynamic properties of DNA circular molecules are investigated by a new path integral computational method which treats in the real space the fundamental forces stabilizing the molecule. The base pair and stacking contributions to the classical action are evaluated separately by simulating a broad ensemble of twisted conformations.
openaire +4 more sources
Rational tangle surgery and Xer recombination on catenanes
, 2011 The protein recombinase can change the knot type of circular DNA. The action of a recombinase converting one knot into another knot is normally mathematically modeled by band surgery.
Conway +5 more
core +1 more source