Results 31 to 40 of about 762,695 (253)

Structural basis for CRISPR RNA-guided DNA recognition by Cascade [PDF]

, 2011
The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids.
A Bolotin, A Noy, AJR Heck, AL Gnatt, Albert J R Heck, AM Maxam, Ambrosius P L Snijders, Arjan Barendregt, B Wiedenheft, Blake Wiedenheft, C Hale, C Pourcel, CR Hale, D Han, DH Haft, DY Lee, Edze R Westra, Egbert J Boekema, Esther van Duijn, FJ Mojica, FJ Mojica, FV Karginov, GT Oostergetel, H Deveau, H Deveau, J Carte, J Li, J van der Oost, JB Ma, JE Garneau, Jelle B Bultema, Jennifer A Doudna, John van der Oost, K Lorenzen, K Yu, Kaihong Zhou, KS Makarova, LA Marraffini, LA Marraffini, LA Marraffini, M Thomas, M van Heel, M Zhou, Magnus Lundgren, Marieke R Beijer, Mark J Dickman, Matthijs M Jore, MJ Dickman, MR Singleton, N Tahallah, P Horvath, P Horvath, R Barrangou, R Jansen, RE Haurwitz, Reinhild Wurm, RH van den Heuvel, RK Lillestøl, Rolf Wagner, S Houwing, Sakharam P Waghmare, SC Raghavan, SJ Brouns, SP Waghmare, Stan J J Brouns, T Itoh, T Wilson-Sali, TA Baker, TH Tang, V Kunin, Y Agari, Ümit Pul   +71 more
core   +5 more sources

RNA-programmed genome editing in human cells

eLife, 2013
Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign DNA at specific sites. We show here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA ...
Martin Jinek, Alexandra East, Aaron Cheng, Steven Lin, Enbo Ma, Jennifer Doudna   +5 more
doaj   +1 more source

DNA multi-bit non-volatile memory and bit-shifting operations using addressable electrode arrays and electric field-induced hybridization. [PDF]

, 2018
DNA has been employed to either store digital information or to perform parallel molecular computing. Relatively unexplored is the ability to combine DNA-based memory and logical operations in a single platform.
Heller, Michael J, Huang, Xiaohua, Kim, Sejung, Song, Youngjun   +3 more
core   +3 more sources

Simplified detection of the hybridized DNA using a graphene field effect transistor

Science and Technology of Advanced Materials, 2017
Detection of disease-related gene expression by DNA hybridization is a useful diagnostic method. In this study a monolayer graphene field effect transistor (GFET) was fabricated for the detection of a particular single-stranded DNA (target DNA).
Arun Kumar Manoharan, Shanmugavel Chinnathambi, Ramasamy Jayavel, Nobutaka Hanagata   +3 more
doaj   +1 more source

Complexation of norfloxacin with DNA in the presence of caffeine [PDF]

, 2006
1H NMR spectroscopy (500 MHz) has been used to quantify the complexation of the antibacterial antibiotic Norfloxacin (NOR) with DNA in the presence of Caffeine (CAF). Separate studies have been made for the self-association of NOR, its hetero-association
Davies, David B., Evstigneev, M.P., Rybakova, K.A.   +2 more
core   +1 more source

Ion-Channel Genosensor for the Detection of Specific DNA Sequences Derived from Plum Pox Virus in Plant Extracts

Sensors, 2014
A DNA biosensor for detection of specific oligonucleotides sequences of Plum Pox Virus (PPV) in plant extracts and buffer is proposed. The working principles of a genosensor are based on the ion-channel mechanism. The NH2-ssDNA probe was deposited onto a
Kamila Malecka, Lech Michalczuk, Hanna Radecka, Jerzy Radecki   +3 more
doaj   +1 more source

Fabrication and functionalization of PCB gold electrodes suitable for DNA-based electrochemical sensing [PDF]

, 2014
The request of high specificity and selectivity sensors suitable for mass production is a constant demand in medical research. For applications in point-of-care diagnostics and therapy, there is a high demand for low cost and rapid sensing platforms ...
Dhaenens, Kristof, Gielen, An, Henry, OYF, Lundeberg, J, O'Sullivan, CK, Salvo, Pietro, Sanchez, JLA, Solnestam, BW, Vanfleteren, Jan   +8 more
core   +1 more source

Impedimetric DNA Biosensor Based on a Nanoporous Alumina Membrane for the Detection of the Specific Oligonucleotide Sequence of Dengue Virus

Sensors, 2013
A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (~50–100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution ...
Chee-Seng Toh, Jiajia Deng
doaj   +1 more source

Identification of DNA-binding protein target sequences by physical effective energy functions. Free energy analysis of lambda repressor-DNA complexes [PDF]

BMC Structural Biology 2007, 7:61, 2007
Specific binding of proteins to DNA is one of the most common ways in which gene expression is controlled. Although general rules for the DNA-protein recognition can be derived, the ambiguous and complex nature of this mechanism precludes a simple recognition code, therefore the prediction of DNA target sequences is not straightforward.
arxiv   +1 more source

Factors Impacting Invader-Mediated Recognition of Double-Stranded DNA

Molecules, 2022
The development of chemically modified oligonucleotides enabling robust, sequence-unrestricted recognition of complementary chromosomal DNA regions has been an aspirational goal for scientists for many decades.
Caroline P. Shepard, Raymond G. Emehiser, Saswata Karmakar, Patrick J. Hrdlicka   +3 more
doaj   +1 more source