Subterranean ecosystems are understudied and challenging to conventionally survey given the inaccessibility of underground voids and networks. In this study, we conducted a eukaryotic environmental DNA (eDNA) metabarcoding survey across the karst ...
Katrina M. West +5 more
doaj +1 more source
Ancient DNA preserved in small bone fragments from the P.W. Lund collection
The Lund collection is one of the oldest subfossil collections in the world. The vast assemblage of subfossils was collected in the 1830s and 1840s by Peter Wilhelm Lund in Lagoa Santa, Brazil, and was shipped to Copenhagen in 1848, where it was stored ...
Frederik V. Seersholm +5 more
doaj +1 more source
Sponges as natural environmental DNA samplers [PDF]
At a time of unprecedented impacts on marine biodiversity, scientists are rapidly becoming persuaded by the potential of screening large swathes of the oceans through the retrieval, amplification and sequencing of trace DNA fragments left behind by marine organisms; an approach known as 'environmental DNA' (eDNA) [1].
Mariani, Stefano +3 more
openaire +4 more sources
Revising the range of Rocky Mountain tailed frog, Ascaphus montanus, in British Columbia, Canada, using environmental DNA methods [PDF]
The Rocky Mountain tailed frog, Ascaphus montanus , is a species at‐risk in Canada. Based upon time‐ and area‐constrained physical search surveys completed between 1996 and 2004, its Canadian distribution was defined as occurring in 19 tributaries and ...
Round, Jessica M. +8 more
core +2 more sources
Ranavirus titers are decoupled in concurrently collected host tissues and environmental DNA samples [PDF]
Please cite as: Alyssa W. Kaganer, Elizabeth M. Bunting, Matthew P. Hare. (2021) Ranavirus titers are decoupled in concurrently collected host tissues and environmental DNA samples. [dataset] Cornell University eCommons Repository.
Kaganer, Alyssa W. +2 more
core +1 more source
Environmental Status Assessment Using DNA Metabarcoding: Towards a Genetics Based Marine Biotic Index (gAMBI) [PDF]
Aylagas, E., Á. Borja, N. Rodríguez-Ezpeleta, 2014. Environmental Status Assessment Using DNA Metabarcoding: Towards a Genetics Based Marine Biotic Index (gAMBI). PLoS ONE, 9(3): e90529.
Rodríguez-Ezpeleta, Naiara +5 more
core +1 more source
Flap endonuclease 1 (FEN1) is an important component of the intricate molecular machinery for DNA replication and repair. FEN1 is a structure-specific 5′ nuclease that cleaves nascent single-stranded 5′ flaps during the maturation of Okazaki fragments ...
Mohamed A. Sobhy +5 more
doaj +1 more source
Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel Internal Amplification Control approach using Biotrove OpenArrays [PDF]
PCR-based detection assays are prone to inhibition by substances present in environmental samples, thereby potentially leading to inaccurate target quantification or false-negative results.
Gent-Pelzer, M. van +13 more
core +1 more source
Phylogenetic classification of short environmental DNA fragments [PDF]
Krause L, Diaz NN, Goesmann A, et al. Phylogenetic classification of short environmental DNA fragments. Nucleic Acids Research. 2008;36(7):2230-2239.Metagenomics is providing striking insights into the ecology of microbial communities.
Nattkemper, Tim Wilhelm ; https://orcid.org/ +10 more
core +2 more sources
The Role of Methylation of DNA in Environmental Adaptation [PDF]
Methylation of DNA is an epigenetic mechanism that influences patterns of gene expression. DNA methylation marks contribute to adaptive phenotypic variation but are erased during development. The role of DNA methylation in adaptive evolution is therefore unclear.
Kevin B, Flores +2 more
openaire +2 more sources

