Results 221 to 230 of about 323,377 (241)
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Fast preparation of fungal DNA for PCR screening
Journal of Microbiological Methods, 2011Rapid DNA preparation for the quick screening is highly demanded in diverse research fields. Here, we combined an extraction buffer and heat treatment to generate DNA templates from yeast and filamentous fungal materials for PCR. This method may be widely applicable to diverse fungal species in clinical and basic studies.
Wei-Chiang Shen +2 more
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Polyamines, DNA Methylation, and Fungal Differentiation
Critical Reviews in Microbiology, 1994Mucorales constitute a group of fungi that, because of their growth characteristics, have been used extensively in the study of cell differentiation, cell morphogenesis, and stimuli perception. We have studied the role of polyamine metabolism in the development of different Mucorales, with emphasis on Mucor and Phycomyces species.
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Relevance of plant and fungal DNA metabarcoding in aerobiology
Aerobiologia, 2019Aerobiology studies biological particles present in the air of both indoor and outdoor environments. In the past few years, the application of molecular techniques has considerably improved the knowledge of organismal diversity in air samples and highlighted its ecological and clinical importance.
BANCHI, ELISA, Pallavicini A., Muggia L.
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Quantitative Analysis of Fungal DNA in Chronic Rhinosinusitis
The Laryngoscope, 2004AbstractObjectives/Hypothesis Fungi have been recognized as important pathogens in sinusitis; however, they are equally present in patients with and without sinusitis. The authors postulated that the quantity of fungal DNA in the nose is determinant of disease, is greater in patients with chronic rhinosinusitis, and is directly correlated to their ...
Andrew H. Murr +4 more
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Tracking Plant, Fungal, and Bacterial DNA in Honey Specimens*
Journal of Forensic Sciences, 2011Abstract: Consuming honey can result in adverse effects owing to poisoning by bacterial (botulism) or plant toxins. We have devised a method to extract polymerase chain reaction (PCR) amplifiable DNA of up to c. 400 bp in length based on dialysis of a 15‐mL honey sample for 18 h against deionized water followed by sequential extraction using phenol ...
OLIVIERI, CRISTINA +3 more
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Nuclear DNA content of 11 fungal species in Glomales
Genome, 1998The nuclear DNA content of 11 species of Glomales was evaluated by flow cytometry after DAPI staining relative to Gigaspora margarita, which was used as internal standard. The nuclear DNA content of this species was calibrated by propidium iodide staining relative to chicken red blood cells.
Hosny, M. +2 more
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Endophytic fungal DNA, the source of contamination in spruce needle DNA
Molecular Ecology, 1997DNA isolated and amplified from higher plants may originate from symbiotic microbes occupying plant tissues. A recent report on the phylogeny of Picea contained sequence data that upon later analysis proved to originate from filamentous ascomycetes. Isolates of endophytic fungi from Picea foliage collected from the same location as the original samples
Jeffrey K. Stone +4 more
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Fungal Nails? DNA Facts Challenge Dystrophic Etiology
Journal of the American Podiatric Medical Association, 2021Abstract Historically recalcitrant to treatment, infection of the nail unit is a pervasive clinical condition affecting about 10%-20% of the U.S. population; patients present with both cosmetic symptomatology and pain, with subsequent dystrophic morphology. To date, the presumptive infectious etiologies include classically-reported fungal dermatophytes,
Beth, Pearce +3 more
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DNA barcodes for marine fungal identification and discovery
Fungal Ecology, 2013Abstract We employed DNA barcodes for identification of fungal species in marine sediments. Sediments were collected seasonally along the Southeast coast of India from which a culturable fungal library was constructed. All cultured species were morphologically documented using microscopical analysis. A maximum population density of 19.3 × 10 3 CFU/g
Seerangan Manokaran +6 more
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Multipartite structure of mitochondrial DNA in a fungal longlife mutant
Plasmid, 1989Rearrangements in the mitochondrial genome of the longlife mutant ex1 of the ascomycete Podospora anserina have led to a heterogeneous population of subgenomic molecules. The restriction maps of individual subcircles were established using overlapping recombinant lambda phages isolated from an ex 1 mtDNA/EMBL3 library.
Karl Esser, Erika Schulte, Ulrich Kück
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