Results 121 to 130 of about 3,781,819 (347)

Measurement of Epstein-Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye [PDF]

, 2016
BACKGROUND Reactivation of Epstein-Barr virus (EBV) infection may cause serious, life-threatening complications in immunocompromised individuals. EBV DNA is often detected in EBV-associated disease states, with viral load believed to be a reflection of ...
Ausimmune Investigator Group (AIG), Dwyer, Dominic E, Lay, Meav-Lang J, Lucas, Robyn, Ponsonby, Anne-Louise, Ratnamohan, Mala, Taylor, Janette   +6 more
core   +1 more source

Measuring turnover of SIV DNA in resting CD4+ T cells using pyrosequencing: implications for the timing of HIV eradication therapies.

PLoS ONE, 2014
Resting CD4+ T cells are a reservoir of latent HIV-1. Understanding the turnover of HIV DNA in these cells has implications for the development of eradication strategies.
Jeanette C Reece, Alexey Martyushev, Janka Petravic, Andrew Grimm, Shayarana Gooneratne, Thakshila Amaresena, Robert De Rose, Liyen Loh, Miles P Davenport, Stephen J Kent   +9 more
doaj   +1 more source

Diffusion‐based size determination of solute particles: a method adapted for postsynaptic proteins

FEBS Open Bio, EarlyView.
We present a diffusion‐based approach for measuring the size of macromolecules and their complexes, and demonstrate its use on postsynaptic proteins. The method requires fluorescein‐labelled protein samples, a microfluidic device that maintains laminar flow for said samples, a microscope recording the emitted fluorescent signals, and an analytic ...
András László Szabó, Eszter Nagy‐Kanta, Soma Varga, Edit Andrea Jáger, Csaba István Pongor, Mária Laki, András József Laki, Zoltán Gáspári   +7 more
wiley   +1 more source

The Cytosolic DNA-Sensing cGAS-STING Pathway in Cancer.

Cancer Discovery, 2019
The recognition of DNA as an immune-stimulatory molecule is an evolutionarily conserved mechanism to initiate rapid innate immune responses against microbial pathogens. The cGAS-STING pathway was discovered as an important DNA-sensing machinery in innate
J. Kwon, S. Bakhoum
semanticscholar   +1 more source

Mitochondria‐associated membranes (MAMs): molecular organization, cellular functions, and their role in health and disease

FEBS Open Bio, EarlyView.
Mitochondria‐associated membranes (MAMs) are contact sites between the endoplasmic reticulum and mitochondria that regulate calcium signaling, lipid metabolism, autophagy, and stress responses. This review outlines their molecular organization, roles in cellular homeostasis, and how dysfunction drives neurodegeneration, metabolic disease, cancer, and ...
Viet Bui, Maryline Santerre, Natalia Shcherbik, Bassel E Sawaya   +3 more
wiley   +1 more source

Genetic diversity in the env V1-V2 region of proviral quasispecies from long-term controller MHC-typed cynomolgus macaques infected with SHIVSF162P4cy [PDF]

, 2018
Intra-host evolution of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) has been shown by viral RNA analysis in subjects who naturally suppress plasma viremia to low levels, known as controllers.
Borsetti, Alessandra, Capone, Alessia, Cella, Eleonora, Ciccozzi, Massimo, Ensoli, Barbara, Farcomeni, Stefania, Ferrantelli, Flavia, Lo Presti, Alessandra, Maggiorella, Maria T, Mee, Edward T, Rose, Nicola J, Sernicola, Leonardo   +11 more
core   +1 more source

The C4 protein encoded by tomato leaf curl Yunnan virus reverses transcriptional gene silencing by interacting with NbDRM2 and impairing its DNA-binding ability

PLoS Pathogens, 2020
In plants, cytosine DNA methylation is an efficient defense mechanism against geminiviruses, since methylation of the viral genome results in transcriptional gene silencing (TGS).
Yuzhen Mei, Yaqin Wang, Fangfang Li, Xueping Zhou, Savithramma P. Dinesh-Kumar   +4 more
doaj  

Interplay between RNA‐protein interactions and RNA structures in gene regulation

FEBS Open Bio, EarlyView.
Methodological advances in mapping transcriptome‐wide RNA‐protein interactions and RNA structures have started to uncover the potential of RNP conformations in gene regulation. Competing RNA–RNA, RNA‐protein and protein–protein interactions shape the compaction and function of RNPs throughout their lifetime and may provide novel therapeutic targets in ...
Jenni Rapakko, Mauro Scaravilli, Minna‐Liisa Änkö   +2 more
wiley   +1 more source

Intracellular sensing of viral genomes and viral evasion

Experimental and Molecular Medicine, 2019
During viral infection, virus-derived cytosolic nucleic acids are recognized by host intracellular specific sensors. The efficacy of this recognition system is crucial for triggering innate host defenses, which then stimulate more specific adaptive ...
Hyun-Cheol Lee, Kiramage Chathuranga, Jong-Soo Lee   +2 more
semanticscholar   +1 more source

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source