Zhongguo shiyan zhenduanxue, 2000 ９０年代初，由美国Ａｆｆｙｍｅｔｒｉｘ公司的Ｆｏｄｏｒ博士提出并开始基因芯片技术的研究。至今，ＤＮＡ芯片技术在医学各个 领域中已显示出巨大的发展潜力并取得一定成功，他以一次性检查上万个基因活动的优势相继在人类肿瘤疾病的研究、艾 滋病的研究、细菌感染患者的研究、遗传病的研究等临床医学各个领域中取得了巨大突破，并且成形的己用于临床诊断的 ＤＮＡ芯片已在１９９７年后相继问世并投入商业化生产。此技术对患者的发病机制，临床诊断，患者的预后及监控患者在用药 方面的临床反应，制定患者的治疗方案中发挥了极大的作用 ...
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[Liquid Biopsy Revolutionizes the Precise Management of Tumors Across the Entire Course: Current Situation and Future Prospects]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanZeng J, Zhang J.
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[Construction of a highly efficient HBV infection cell model based on HBV co-receptor neuropilin-1]. [PDF]
Zhonghua Gan Zang Bing Za ZhiLiu ZH, He XM, Xu KX, Yu HB, Chen J.
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[<i>Streptococcus salivarius</i>-derived extracellular vesicles exacerbate progression of lupus nephritis by activating natural killer cells]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoXiao J, Jin L, Duan L, Gong Y, Li H.
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Zhongshan Daxue xuebao. Yixue kexue ban, 1994 以异硫氰酸胍裂解血清释放核酸使被二氧化硅吸附，经乙醇洗涤后用水悬浮，上清即含纯化ＤＮＡ。结合双阶变温ＰＣＲ建立一种简单快速的血清ＨＢＶ－ＤＮＡ检测方法。该法只需检测１０ｕｌ血清，完成提取和扩增只要３ｈ．操作很易掌握。用之检测５６例ＨＢｓＡｇ＋／ＨＢｅＡｇ＋的血清、４２例ＨＢｓＡｇ＋／ＨＢｅＡｇ－的血清和５４例ＨＢｓＡｇ／ＨＢｅＡｇ的血清，结果ＨＢＶ－ＤＮＡ检出率分别为８６％、５７％和１３％。应用表明这是一种简捷有效的核酸纯化和扩增方法，适于对大量临床样本作检测之用。
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[Differential analysis of gut microbiome in patients with periprosthetic joint infection, aseptic failure, and osteoarthritis]. [PDF]
Zhongguo Xiu Fu Chong Jian Wai Ke Za ZhiMuzhabaier K +6 more
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[ROMO1 Promotes the Repair of Radiation-induced Skin Injury by Regulating Mitochondrial Function and Keratinocyte Proliferation]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanYang T +8 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 1993 用多聚酶链反应(PCR)技术检测了16例肝细胞性肝癌(HCC)患者癌灶和癌旁双份组织石蜡包埋标本中的乙型肝炎病毒脱氧核糖核酸(HBV-DNA)。以C基因引物扩增HBV-DNA,32份标本中检出率71.87％。以新鲜冷冻肝标本作对照检出率81.25％,二者相比无显著性差别(X~=0.57,P>0.05)。以质粒提取HBV-DNA作灵敏度测定,PCR-EB可达10fg,PCR-SBH可达lag。应用非放射性的地高辛素标记探针作杂交灵敏度达~32P标记探针的水平。
doaj
[Retrospect and Prospect of Immune Caries Prevention Research in China]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanXu Q, Fan M.
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[Screening of High-quality Spermatozoa Using Transwell Membrane Pores and Assessment of Their Performance]. [PDF]
Sichuan Da Xue Xue Bao Yi Xue BanLi Y +7 more
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