Results 11 to 20 of about 4,740 (167)

Climate stability drives multidimensional nestedness of amphibian assemblages in a Chinese sky island system

open access: yesFunctional Ecology, EarlyView.
Read the free Plain Language Summary for this article on the Journal blog. Abstract The nested subset pattern (nestedness) has been widely used to explain species distributions in island and fragmented systems. Mountain sky islands serve as critical natural laboratories for understanding the evolutionary consequences of geographic isolation and climate
Caiwen Zhang   +5 more
wiley   +1 more source

Tolerability and Feasibility of Minimally Invasive Canine Skin Sampling: Excellent Tolerability Meets Transcriptomic Challenges

open access: yesVeterinary Dermatology, EarlyView.
ABSTRACT Background Advances in transcriptomics have driven the demand for minimally invasive, reproducible and high‐yield skin sampling methods, particularly for studying inflammatory skin diseases in companion animals. Hypothesis/Objectives We tested tolerability, feasibility and RNA quantity and quality of three minimally invasive skin sampling ...
Ina Herrmann   +2 more
wiley   +1 more source

The genetic diversity of mango (Mangifera indica L.) germplasm in Taiwan by high‐throughput single‐nucleotide polymorphism genotyping

open access: yesPLANTS, PEOPLE, PLANET, Volume 8, Issue 1, Page 301-315, January 2026.
Mango, the king of fruits, is important to daily life, nutrition, and households in South and Southeast Asia. It is considered the second most important fruit in Taiwan, where many varieties are available. Using high‐throughput DNA genotyping, we analyzed mango germplasm for variety identification, genebank management, and breeding. Our results confirm
Ching‐heng Lin   +5 more
wiley   +1 more source

DNA Barcoding Reveals Cryptic Diversity and Informs Conservation Priorities in Chinese Firmiana Species (Malvaceae) Using Genome Skimming Data DNA条形码揭示中国梧桐属 (锦葵科) 隐存多样性及保护优先级:来自基因组浅层测序数据的证据

open access: yesIntegrative Conservation, Volume 4, Issue 4, Page 569-583, December 2025.
Accurate species identification is critical for conserving endangered Firmiana trees in China. However, morphological similarities among closely related Firmiana species complicate its taxonomic identification. We conducted genome skimming on 62 samples representing all 10 Chinese species and discovered nrITS as the optimal DNA barcode, achieving 100 ...
Li‐Jun Yan   +8 more
wiley   +1 more source

Recommendations to Reinforce the Implementation of China's Updated National Key Protected Wild Plants List 关于强化实施中国新版《国家重点保护野生植物名录》的建议

open access: yesIntegrative Conservation, Volume 4, Issue 4, Page 527-533, December 2025.
The recommendations cover four key aspects namely the definition of the taxa require high priority in the biodiversity conservation management, enhanced flexibility and adaptivity of the implementation processes, enforced integration of research advancement to enable the sustainable usage of plant resources, and finally actions to propose transboundary
Shi‐Cheng Shao   +4 more
wiley   +1 more source

How to talk about crises? Leaders' narrative strategies during the COVID‐19 vaccination campaign in Italy and France

open access: yesPolicy Studies Journal, Volume 53, Issue 4, Page 994-1013, November 2025.
Abstract Defined by threat, urgency, and uncertainty, crises produce opportunities for government leaders to exploit and create meaning around their policy decisions in such unstable circumstances. In narrating their preferred policy solutions, one of the tools governments can use is relying on evidence‐based information.
Laura Mastroianni, Stefania Profeti
wiley   +1 more source

Effect of a ceruminolytic ear cleaner on clinical, microbiological and ear canal microbiome evolution in canine erythemato‐ceruminous otitis externa associated with proliferation of Malassezia yeasts

open access: yesVeterinary Dermatology, Volume 36, Issue 5, Page 668-678, October 2025.
Background – Erythemato‐ceruminous otitis externa (ECOE) is commonly associated with Malassezia spp. and microbial imbalance. Hypothesis/Objective – To assess the clinical performance of an ear cleaner in dogs suffering from ECOE associated with Malassezia spp. overgrowth and to measure its impact on the microbiota. Conclusions and Clinical Relevance –
Amaury Briand   +11 more
wiley   +1 more source

Genetic mapping and quantitative trait loci analysis of growth-related traits in the small abalone Haliotis diversicolor using next-generation sequencing [PDF]

open access: yes, 2016
杂色鲍(Haliotisdiversicolor)广泛分布于韩国、日本、中国、越南和菲利宾沿岸,是一种具有重要经济价值的养殖鲍种。应用传统的育种方式,杂色鲍育种工作虽然已经取得了一定进展,但是开展分子标记辅助选育仍然是加速该进程的必然选择。下一代测序技术可以高通量、低成本地完成SNP标记的发掘与分型,目前已被广泛应用于遗传研究的各个方面。本文利用下一代测序技术完成了杂色鲍的SNP标记开发与验证、高密度连锁图谱构建、QTL定位和候选基因的关联分析等工作。主要结果如下: (1) 杂色鲍高密度连锁图谱的构建 ...
任鹏
core  

高海拔地区血液基因组DNA提取条件优化

open access: yesZhongguo shiyan zhenduanxue, 2018
目的探讨在高海拔地区应用血液基因组DNA提取试剂盒的最佳条件。方法应用RelaxGene Blood DNA System血液基因组DNA提取系统,对109份血样(每份血样分为2组,实验组和对照组)提取DNA,实验组采用优化条件提取DNA、对照组采用试剂盒说明书方法提取DNA。结果实验组109份样品中有13份DNA浓度在20-50ng/μL之间,35份在51-80ng/μL之间,61份样品>80ng/μL;DNA浓度比例高于对照组 ...
范东艳, 杜宝中, 王苹, 全雄杰
doaj  

A rapid soil DNA extraction method applied to field detection(一种适用于现场检测的土壤DNA快速提取方法(英文))

open access: yes浙江大学学报. 农业与生命科学版
Soil DNA extraction, such as microbial community analysis and gene drift detection, is an important basis for multiple analyses in different fields. Nevertheless, the soil DNA extraction methods for field detection are still lacking.
YAN Jingying(颜晶莹)   +3 more
doaj   +1 more source

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