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野生动物学报, 2020 毛发（无根）作为一种非损伤性的生物样本，在保护遗传学和分子生态学研究中发挥着十分重要的作用。以毛干中提取的DNA为模板做PCR扩增时，mtDNA片段扩增效果较好，但核基因的扩增成功率极低。人们将其归因于核DNA质低量少所致。然而我们在前期研究中发现，从毛干中得到的总DNA混有大量的细菌DNA，是导致核基因扩增困难的主要因素之一。为此我们尝试建立一种高效、快速、低成本，而且不影响DNA提取的清除毛干细菌的方法。本研究以蓝狐被毛为材料，经过普通清洗后，以4种方法处理毛干，随后使用磁珠法进行总DNA提取 ...
周永恒 1 马跃 1 +3 more
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野生动物学报, 2010 褐马鸡是中国特有的国家Ⅰ级保护濒危鸟类,为了解决野生褐马鸡分子生物学研究中存在的取样局限性,采用非伤害性取样和非损伤性取样,对褐马鸡微量血液、皮、脚垫、指甲、羽毛、陈旧标本的皮以及粪便的DNA提取进行了初步研究。结果表明,从褐马鸡微量血液（10μL）中可以提取到浓度和纯度高的基因组DNA（41.85μg）;从褐马鸡的皮、脚垫、指甲、羽毛中可以提取出基因组DNA并扩增出目标片断;陈旧标本的皮能够提取到基因组DNA。该研究将拓宽褐马鸡分子生物学研究的采样范围,并可以提高野外非损伤性取样在保护遗传学中的应用。
武玉珍, 王孟本, 张峰
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Implications of Tobacco Use on Pathogen‐Driven Diseases: A Public Health and Policy Perspective
World Medical &Health Policy, Volume 17, Issue 3, Page 468-474, September 2025.ABSTRACT The tobacco epidemic remains one of the most significant global public health challenges, responsible for over 8 million deaths annually, with a substantial portion occurring among non‐smokers exposed to second‐hand smoke. While cigarette smoking remains the most prevalent form of tobacco use worldwide, the rise of electronic nicotine delivery
Nadine Kabbani, James L. Olds
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Zhongguo shiyan zhenduanxue, 2013 目的比较两种不同方法提取的基因组DNA的纯度、产量和后续实验效果。方法采用酚/氯仿法和盐析法两种方法直接从全血中提取基因组DNA,并采用电泳、PCR和基质支持的激光释放/电离飞行时间质谱分析（MALDI-TOF MS）方法进行检测。结果酚/氯仿法提取的基因组DNA纯度高于盐析法 ...
宋洁云 +4 more
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Veterinary Dermatology, Volume 36, Issue 4, Page 474-484, August 2025.Background – Sporotrichosis is a chronic, mycotic infection caused by fungi of the genus Sporothrix. Zoonotic sporotrichosis occurs mainly through S. brasiliensis transmission, resulting from the organism's traumatic introduction via scratches or bites, or contact with exudate from contaminated cats.
Steffanie Amadei +6 more
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Systematic Entomology, Volume 50, Issue 3, Page 495-518, July 2025.Using genomic data from 285 species across 19 planthopper families, we conducted the first phylogenomic study targeting global planthoppers and inferred their phylogeny with the highest resolution so far. We proposed taxonomic changes including the establishment of a new family Borysthenidae stat. rev. and a new superfamily Meenoploidea superfam.
Junchen Deng +9 more
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Zhongguo shiyan zhenduanxue, 2003 目的 探讨石蜡包埋组织中提取DNA应用于PCR反应的影响因素。方法 应用从石蜡包埋组织中提取的DNA ,PCR反应扩增甲状腺髓样癌RET基因第 11外显子 ,分析石蜡包埋组织中提取DNA应用于PCR反应的影响因素。结果 10 .0 μm× 1组织切片组 ,PCR反应循环次数 4 0次组 ,PCR反应模板量 0 .0 5 μg组PCR反应取得高质量目的基因DNA。结论 减少提取组织用量有利于减少抑制PCR物质的量 ,有利于反应的成功 ;PCR反应循环次数一般应大于 4 0次 ...
赵吉生 +6 more
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Large seeds as a defensive strategy against partial granivory in the Fagaceae
Journal of Ecology, Volume 113, Issue 3, Page 598-607, March 2025.Large seeds dispersed by scatter‐hoarding granivores, such as Fagaceae acorns, tolerate partial damage to achieve higher dispersal efficiency. By demonstrating that seed size itself acts as a defensive trait, the findings provide new insights into seed size diversity and evolution, highlighting how large seeds counterbalance reserve losses to evade ...
Si‐Chong Chen +5 more
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研磨-CTAB法与碱性异硫氰酸胍沸腾法提取真菌DNA的比较分析
Zhongguo shiyan zhenduanxue, 2005 目的对两种病原真菌基因组DNA提取方法[研磨-CTAB（十六烷基三甲基溴化胺）法及碱性异硫氰酸胍沸腾法]的提取效果进行比较,为临床真菌标本的前期处理提供有效参考。方法对来自于同一批培养基的4株申克孢子丝菌及6株普通真菌菌株分别采用研磨-CTAB法及碱性异硫氰酸胍沸腾法抽提基因组DNA,并用紫外分光光度计测定基因组DNA样品OD260和OD280值,计算提取样品的浓度并估计核酸的纯度,然后采用配对资料t检验的统计学方法进行比较。结果碱性异硫氰酸胍沸腾法提取的DNA浓度及纯度均高于研磨-CTAB法 ...
李晓红 +4 more
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European Policy Analysis, Volume 11, Issue 1, Page 114-137, Winter 2025.Abstract In Europe, and specifically in countries bordering the Baltic Sea, preparedness issues have moved up the policy agenda since Russia's 2014 and 2022 invasions of Ukraine. “Ordinary people” are encouraged to prepare for crises—be it through stockpiling food at home, fact‐checking information, or signing up for military training.
Alexandra M. Friede
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