Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A subcellular selective APEX2-based proximity labeling used for identifying mitochondrial G-quadruplex DNA binding proteins. [PDF]
Wang X +5 more
europepmc +1 more source
DNAPred_Prot: Identification of DNA-Binding Proteins Using Composition- and Position-Based Features. [PDF]
Barukab O, Khan YD, Khan SA, Chou KC.
europepmc +1 more source
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
A bacterial expression cloning screen reveals single-stranded DNA-binding proteins as potent desicco-protectants. [PDF]
Hibshman JD +3 more
europepmc +1 more source
Comparative Analysis on Alignment-Based and Pretrained Feature Representations for the Identification of DNA-Binding Proteins. [PDF]
Chen D, Zhang H, Chen Z, Xie B, Wang Y.
europepmc +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Regulation of DNA damage response by RNA/DNA-binding proteins: Implications for neurological disorders and aging. [PDF]
Kodavati M +3 more
europepmc +1 more source
Inhibitor of DNA binding proteins revealed as orchestrators of steady state, stress and malignant hematopoiesis. [PDF]
Singh S +6 more
europepmc +1 more source

