Results 191 to 200 of about 1,668,835 (315)

Base-resolution UV footprinting by sequencing reveals distinctive damage signatures for DNA-binding proteins. [PDF]

Nat Commun, 2023
Elliott K, Singh VK, Boström M, Larsson E.   +3 more
europepmc   +1 more source

PANoptosis in the pathogenesis of myelodysplastic syndromes

Molecular Oncology, EarlyView.
PANoptosis, a combination of three types of programmed cell death, is mediated by a large protein complex called a PANoptosome. In healthy bone marrow hematopoietic cells, PANoptosis is restricted by inhibitory signaling. In MDS, bone marrow cells become sensitive to the PANoptotic stimuli due to the aberrant inactivation of inhibitory signaling or ...
Rohit Thalla, Jyoti Lamichhane, Cameron Lewis, Peter Breslin, Jiwang Zhang   +4 more
wiley   +1 more source

Multiple RNA- and DNA-binding proteins exhibit direct transfer of polynucleotides with implications for target-site search. [PDF]

Proc Natl Acad Sci U S A, 2023
Hemphill WO, Voong CK, Fenske R, Goodrich JA, Cech TR.   +4 more
europepmc   +1 more source

Spatial biology in cancer epigenetics

Molecular Oncology, EarlyView.
Spatial epigenomics combines molecular profiling with tissue architecture to reveal how gene regulation is organized within intact tissues. In cancer, these technologies uncover the mechanisms driving tumor heterogeneity and microenvironmental interactions, opening new opportunities for biomarker discovery and precision medicine.
Eva Crespo‐García, Manel Esteller
wiley   +1 more source

TransBind allows precise detection of DNA-binding proteins and residues using language models and deep learning. [PDF]

Commun Biol
Tahmid MT, Hasan AKMM, Bayzid MS.
europepmc   +1 more source

Hybrid_DBP: Prediction of DNA-binding proteins using hybrid features and convolutional neural networks. [PDF]

Front Pharmacol, 2022
Yu S, Peng D, Zhu W, Liao B, Wang P, Yang D, Wu F.   +6 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Single-molecule fluorescence reveals the DNA unwinding mechanism of mitochondrial helicase TWINKLE and its interplay with single-stranded DNA-binding proteins. [PDF]

Nucleic Acids Res
Wang HY, Lee YY, Chien PJ, Tsai WA, Sun PJ, Wang LT, Wu CC, Fan HF.   +7 more
europepmc   +1 more source

Single-Stranded DNA Binding Proteins and Their Identification Using Machine Learning-Based Approaches. [PDF]

Biomolecules, 2022
Guo JT, Malik F.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source