Results 261 to 270 of about 5,403,999 (308)

Computational Design of DNA-Binding Proteins

2016
Predicting the outcome of engineered and naturally occurring sequence perturbations to protein-DNA interfaces requires accurate computational modeling technologies. It has been well established that computational design to accommodate small numbers of DNA target site substitutions is possible. This chapter details the basic method of design used in the
Summer, Thyme, Yifan, Song
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Affinity purification of DNA-binding proteins

Journal of Biochemical and Biophysical Methods, 2001
The focus of this review is on DNA affinity chromatography, which is the most powerful tool for purification of DNA binding proteins. The use of nonspecific-, sequence specific- and single stranded-DNA affinity columns in purification of various DNA binding proteins is discussed.
H, Gadgil, S A, Oak, H W, Jarrett
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Protein DNA Binding

This lesson explores the structure and function of the restriction enzyme EcoRI. The activity begins by reviewing the RCSB Molecule of the Month article on this class of enzymes and examines interactions of DNA and protein (the enzyme) in order to accomplish a specific function (cutting the DNA molecule at a specific sequence).
Gadd, Brian, Dutta, Shuchismita
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DNA Stability and DNA Binding Proteins

1996
Publisher Summary This chapter reviews the initial studies that focus on characterizing the factors that contribute to the stability of the genomes of hyperthermophiles, both extrinsic factors, such as the intracellular ionic environment and so-called histone like DNA binding proteins, and intrinsic factors, such as DNA base composition and topology.
R A, Grayling, K, Sandman, J N, Reeve
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DNA-Binding proteins in human serum

Biochemical and Biophysical Research Communications, 1975
Summary Human serum DNA-binding proteins were isolated by affinity chromatography on DNA-cellulose. These proteins represent a significant fraction (1.5%) of the serum proteins. The isolated proteins were quantitatively assayed by a nitrocellulose membrane filter technique using human DNA.
S P, Brehm, S O, Hoch, J A, Hoch
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Glycolytic enzymes as DNA binding proteins

International Journal of Biochemistry, 1993
1. Numerous studies have demonstrated the presence of at least four glycolytic enzymes in the nuclear compartment of several cell systems. 2. These include, lactate dehydrogenase, phosphoglycerate kinase, aldolase and glyceraldehyde-3-phosphate dehydrogenase. 3.
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DNA binding of a nonstructural reovirus protein

Canadian Journal of Biochemistry, 1981
The specific early inhibition of DNA synthesis in reovirus-infected cells suggests that the cell nucleus is a target for virus-induced damage. We have now examined the affinity of reovirus proteins for DNA, postulating that such affinity could provide a mechanism for the inhibition.
I H, Shelton, G J, Kasupski, C, Oblin, R, Hand   +3 more
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Electrophoretic assay for DNA-binding proteins.

Methods in enzymology, 1987
This chapter describes a generally applicable assay for specific DNA-binding proteins in crude extracts. The assay is based on gel electrophoretic separation of protein–DNA complexes from each other and from free DNA. This assay employed in several laboratories to detect a variety of DNA-binding proteins.
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Structure and function of DNA-binding proteins

Current Opinion in Genetics & Development, 1995
The past year has seen the determination of over three dozen new structures of DNA-binding proteins. Highlights of new structural motifs include the DNA-binding domain of p53 tumor suppressor and the amino-terminal fragment of Escherichia coli DNA topoisomerase I.
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Molecular Beacons for DNA Binding Proteins: An Emerging Technology for Detection of DNA Binding Proteins and Their Ligands

ASSAY and Drug Development Technologies, 2006
Quantitation of the level or activity of specific proteins is one of the most commonly performed experiments in biomedical research. Protein detection has historically been difficult to adapt to high throughput platforms because of heavy reliance upon antibodies for protein detection.
Benjamin, Dummitt, Yie-Hwa, Chang
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