Results 261 to 270 of about 1,958,390 (304)

Cytoplasmic DNA-binding proteins

Experimental Cell Research, 1973
Abstract Cytoplasmic DNA-binding proteins were isolated from Chinese hamster liver, kidney and tissue culture cells by DNA-polyacrylamide chromatography. With homologous Chinese hamster DNA, and with calf thymus DNA, 1.4% of the proteins were bound to the column. With single-stranded DNA and with heterologous Micrococcus lysodeikticus DNA there was
S T, Vaughan, D E, Comings
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Ultrasensitive protein–DNA binding assays

Current Opinion in Biotechnology, 2003
Protein-DNA binding assays have been used in a variety of fields from fundamental studies regarding the binding process itself, to serving as probes for the detection, quantification and separation of target analytes. These assays have been used for the study of protein-DNA complex stoichiometry, the detection of DNA damage, and real-time separation of
Victor, Pavski, X Chris, Le
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DNA Binding by Proteins

Science, 1988
Study of proteins that recognize specific DNA sequences has yielded much information, but the field is still in its infancy. Already two major structural motifs have been discovered, the helix-turn-helix and zinc finger, and numerous examples of DNA-binding proteins containing either of them are known. The restriction enzyme Eco RI uses yet a different
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Affinity purification of DNA-binding proteins

Journal of Biochemical and Biophysical Methods, 2001
The focus of this review is on DNA affinity chromatography, which is the most powerful tool for purification of DNA binding proteins. The use of nonspecific-, sequence specific- and single stranded-DNA affinity columns in purification of various DNA binding proteins is discussed.
H, Gadgil, S A, Oak, H W, Jarrett
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DNA-Binding proteins in human serum

Biochemical and Biophysical Research Communications, 1975
Summary Human serum DNA-binding proteins were isolated by affinity chromatography on DNA-cellulose. These proteins represent a significant fraction (1.5%) of the serum proteins. The isolated proteins were quantitatively assayed by a nitrocellulose membrane filter technique using human DNA.
S P, Brehm, S O, Hoch, J A, Hoch
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DNA-binding proteins of mammalian mitochondria

Mitochondrion, 2005
Acid-soluble proteins were isolated from liver and spleen mitochondria and their ability to form complexes with DNA was investigated. According to electrophoresis data, acid-soluble proteins include about 20 polypeptides ranging in the molecular mass from 10 to 120 kDa.
Michael P, Kutsyi, Natalia A, Gouliaeva, Elena A, Kuznetsova, Azhub I, Gaziev   +3 more
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α-Fetoprotein gene DNA-binding proteins

Archives of Biochemistry and Biophysics, 1985
The technique of protein blotting was used to study nuclear protein interaction with the alpha-fetoprotein (AFP) gene. The DNA-binding specificity was optimized by varying the amount of competitor DNA and the ionic strength. The specific binding of AFP gene DNA was observed for a set of Morris hepatoma 7777 nuclear proteins. Similar specificity was not
G J, Cote, Z, Wang, J F, Chiu
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Plant bZIP Protein DNA Binding Specificity

Journal of Molecular Biology, 1993
Plant bZIP proteins exhibit a relaxed DNA-binding specificity for DNA sequence motifs containing an ACGT core. Gel mobility shift experiments employing ten different recombinant plant bZIP proteins demonstrated that nucleotides flanking the ACGT core affected binding specificity and identified three different types of ACGT elements: G-box, CACGTG; C ...
T, Izawa, R, Foster, N H, Chua
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Computational Design of DNA-Binding Proteins

2016
Predicting the outcome of engineered and naturally occurring sequence perturbations to protein-DNA interfaces requires accurate computational modeling technologies. It has been well established that computational design to accommodate small numbers of DNA target site substitutions is possible. This chapter details the basic method of design used in the
Summer, Thyme, Yifan, Song
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Glycolytic enzymes as DNA binding proteins

International Journal of Biochemistry, 1993
1. Numerous studies have demonstrated the presence of at least four glycolytic enzymes in the nuclear compartment of several cell systems. 2. These include, lactate dehydrogenase, phosphoglycerate kinase, aldolase and glyceraldehyde-3-phosphate dehydrogenase. 3.
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