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α-Fetoprotein gene DNA-binding proteins
Archives of Biochemistry and Biophysics, 1985The technique of protein blotting was used to study nuclear protein interaction with the alpha-fetoprotein (AFP) gene. The DNA-binding specificity was optimized by varying the amount of competitor DNA and the ionic strength. The specific binding of AFP gene DNA was observed for a set of Morris hepatoma 7777 nuclear proteins. Similar specificity was not
G J, Cote, Z, Wang, J F, Chiu
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Plant bZIP Protein DNA Binding Specificity
Journal of Molecular Biology, 1993Plant bZIP proteins exhibit a relaxed DNA-binding specificity for DNA sequence motifs containing an ACGT core. Gel mobility shift experiments employing ten different recombinant plant bZIP proteins demonstrated that nucleotides flanking the ACGT core affected binding specificity and identified three different types of ACGT elements: G-box, CACGTG; C ...
T, Izawa, R, Foster, N H, Chua
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Computational Design of DNA-Binding Proteins
2016Predicting the outcome of engineered and naturally occurring sequence perturbations to protein-DNA interfaces requires accurate computational modeling technologies. It has been well established that computational design to accommodate small numbers of DNA target site substitutions is possible. This chapter details the basic method of design used in the
Summer, Thyme, Yifan, Song
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Glycolytic enzymes as DNA binding proteins
International Journal of Biochemistry, 19931. Numerous studies have demonstrated the presence of at least four glycolytic enzymes in the nuclear compartment of several cell systems. 2. These include, lactate dehydrogenase, phosphoglycerate kinase, aldolase and glyceraldehyde-3-phosphate dehydrogenase. 3.
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Double-Stranded DNA Binding Protein Hu
1989The genome of eukaryotes is highly organised, structurally and functionally. The supercoiling of the DNA, its packing around the histone core into nucleosomes, and the ordering of nucleosomes into chromatin results in highly condensed chromosomes in the cell nucleus (Felsenfeld and McGhee, 1986).
K, Wilson +3 more
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DNA Stability and DNA Binding Proteins
1996Publisher Summary This chapter reviews the initial studies that focus on characterizing the factors that contribute to the stability of the genomes of hyperthermophiles, both extrinsic factors, such as the intracellular ionic environment and so-called histone like DNA binding proteins, and intrinsic factors, such as DNA base composition and topology.
R A, Grayling, K, Sandman, J N, Reeve
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The adenovirus DNA-binding protein DBP
Journal of VirologyAdenoviruses are a group of double-stranded DNA viruses that can mainly cause respiratory, gastrointestinal, and eye infections in humans. In addition, adenoviruses are employed as vector vaccines for combatting viral infections, including SARS-CoV-2, and serve as excellent gene therapy vectors.
Luca D. Bertzbach +5 more
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Telomeric DNA binding proteins.
BioEssays : news and reviews in molecular, cellular and developmental biology, 1994The physical ends of eukaryotic chromosomes form a specialized nucleoprotein complex composed of DNA and DNA binding proteins. This nucleoprotein complex, termed the telomere, is essential for chromosome stability. In most organisms, the DNA portion of the nucleo-protein complex consists of simple tandem DNA repeats with one strand guanine rich.
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