Results 81 to 90 of about 1,985,752 (333)

Electrical Probes of DNA-Binding Proteins [PDF]

, 2017
A DNA electrochemistry platform has been developed to probe proteins bound to DNA electrically. Here gold electrodes are modified with thiol-modified DNA, and DNA charge transport chemistry is used to probe DNA binding and enzymatic reaction both with redox-silent and redox-active proteins.
Barton, Jacqueline K., Bartels, Phillip L., Deng, Yingxin, O'Brien, Elizabeth   +3 more
openaire   +3 more sources

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

\u3cem\u3ePlasmodium falciparum\u3c/em\u3e SSB Tetramer Wraps Single-Stranded DNA with Similar Topology but Opposite Polarity to \u3cem\u3eE. coli\u3c/em\u3e SSB [PDF]

, 2012
Single-stranded DNA binding (SSB) proteins play central roles in genome maintenance in all organisms. Plasmodium falciparum, the causative agent of malaria, encodes an SSB protein that localizes to the apicoplast and likely functions in the replication ...
Antony, Edwin, Korolev, Sergey, Lohman, Timothy M., Weiland, Elizabeth A.   +3 more
core   +1 more source

How does a protein search for the specific site on DNA: the role of disorder

, 2006
Proteins can locate their specific targets on DNA up to two orders of magnitude faster than the Smoluchowski three-dimensional diffusion rate. This happens due to non-specific adsorption of proteins to DNA and subsequent one-dimensional sliding along DNA.
B. I. Shklovskii, B. I. Shklovskii, M. Ptashne, M. Slutsky, P. H. Richter, Tao Hu   +5 more
core   +1 more source

Control of DNA minor groove width and Fis protein binding by the purine 2-amino group. [PDF]

, 2013
The width of the DNA minor groove varies with sequence and can be a major determinant of DNA shape recognition by proteins. For example, the minor groove within the center of the Fis-DNA complex narrows to about half the mean minor groove width of ...
Cascio, Duilio, Di Felice, Rosa, Ghane, Tahereh, Hancock, Stephen P, Johnson, Reid C, Rohs, Remo   +5 more
core   +2 more sources

Two hypervarlable minisatellite DNA binding proteins

Nucleic Acids Research, 1991
Hypervariable minisatellite DNA sequences are short, tandemly repeated sequences present at numerous loci in eukaryotes. They stimulate intermolecular homologous recombination up to 13-fold in human cells in culture and may be specific sites for the initiation of recombination in the eukaryotic genome (Wahls, W.P., Wallace, L.J., & Moore, P.D.
W P, Wahls, G, Swenson, P D, Moore
openaire   +3 more sources

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Effective Distance for DNA-Mediated Charge Transport between Repair Proteins [PDF]

, 2019
The stacked aromatic base pairs within the DNA double helix facilitate charge transport down its length in the absence of lesions, mismatches, and other stacking perturbations.
Barton, Jacqueline K., Bedoya, Sebastian, Tse, Edmund C. M., Zwang, Theodore J.   +3 more
core   +5 more sources

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

DNA binding compatibility of the Streptococcus pneumoniae SsbA and SsbB proteins. [PDF]

PLoS ONE, 2011
BACKGROUND: Streptococcus pneumoniae has two paralogous, homotetrameric, single-stranded DNA binding (SSB) proteins, designated SsbA and SsbB. Previous studies demonstrated that SsbA and SsbB have different solution-dependent binding mode preferences ...
Brenda Salerno, Geetha Anne, Floyd R Bryant   +2 more
doaj   +1 more source