Results 191 to 200 of about 665,876 (395)

Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity

FEBS Open Bio, EarlyView.
ICP34.5 is one of the most important antihost response proteins. The saRNA‐encoding HSV‐1 neurovirulence protein ICP34.5 clearly mediated the eukaryotic initiation factor 2 alpha subunit (eIF2α) dephosphorylation and significant suppression of innate immune responses in vitro, leading to enhanced expression of the saRNA‐encoded gene.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
wiley   +1 more source

Theory of Nucleosome Corkscrew Sliding in the Presence of Synthetic DNA Ligands [PDF]

arXiv, 2004
Histone octamers show a heat-induced mobility along DNA. Recent theoretical studies have established two mechanisms that are qualitatively and quantitatively compatible with in vitro experiments on nucleosome sliding: Octamer repositiong through one-basepair twist defects and through ten-basepair bulge defects. A recent experiment demonstrated that the
arxiv  

The use of native T7 DNA polymerase for site-directed mutagenesis [PDF]

, 1989
Kataryzna Bebenek, Thomas A. Kunkel
openalex   +1 more source

Comparative study of adenosine 3′‐pyrophosphokinase domains of MuF polymorphic toxins

FEBS Open Bio, EarlyView.
With the ultimate goal of understanding the association of toxin‐immunity modules to temperate phages, we characterized toxins from three prophages and examined cross‐protection from immunity proteins. The toxins exhibit adenosine 3′‐pyrophosphokinase activity and are toxic in Escherichia coli.
Eloïse M. Paulet, Julia Bartoli, Atakan Kabtan, Chloé M. Piras, Audrey C. Tempier, Eric Cascales, Julie P. Viala   +6 more
wiley   +1 more source

Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase

BioTechniques, 2005
Yair Adereth, Kristen J. Champion, Tien Hsu, Vincent Dammai   +3 more
doaj   +1 more source

Direct method for detecting small quantities of hepatitis B virus DNA in serum and plasma using the polymerase chain reaction. [PDF]

, 1989
Jerome B. Zeldis, J H Lee, D. Mamish, D J Finegold, Reema Sircar, Qiu Ling, Peter Juel Thiis Knudsen, I. K. Kuramoto, Larry Mimms   +8 more
openalex   +1 more source

Fungal speciation using quantitative polymerase chain reaction (QPCR) in patients with and without chronic rhinosinusitis. [PDF]

, 2006
Objectives/hypothesisThe objectives of this study were to determine the mycology of the middle meatus using an endoscopically guided brush sampling technique and polymerase chain reaction laboratory processing of nasal mucous; to compare the mycology of ...
Goldberg, Andrew N, Murr, Andrew H, Vesper, Stephen   +2 more
core  

BEAN and HABAS: Polyphyletic insertions in the DNA‐directed RNA polymerase

Protein Science
AbstractThe β and β′ subunits of the RNA polymerase (RNAP) are large proteins with complex multi‐domain architectures that include several insertional domains. Here, we analyze the domain organizations of RNAP‐β and RNAP‐β′ using sequence, experimentally determined structures and AlphaFold structure predictions.
Alvarez‐Carreño, Claudia, Huynh, Angela T., Petrov, Anton S., Orengo, Christine, Williams, Loren Dean   +4 more
openaire   +2 more sources

Iron‐dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP‐1 monocytes

FEBS Open Bio, EarlyView.
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna, Richard Buchal, Martin Leníček, Amit Shachak, Jan Pláteník   +4 more
wiley   +1 more source

FEM1B enhances TRAIL‐induced apoptosis in T lymphocytes and monocytes

FEBS Open Bio, EarlyView.
FEM1B facilitates TRAIL‐induced apoptosis through distinct mechanisms in T lymphocytes and monocytes. In T lymphocytes, FEM1B engages with TRAF2, leading to a reduction in TRAF2 expression, which subsequently lessens TRAF2's inhibitory influence on caspase‐8.
Chenbo Yang, Wenhui Yu, Cui Dang, Jingjing Zhang, Jiahan Lu, Jing Xue   +5 more
wiley   +1 more source