Results 71 to 80 of about 416,069 (337)

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke, AHN Hopman, AR Mitchell, C Huxley, C Lengauer, Christoph Lengauer, D Pinkel, ED Green, EW Jabs, HF Willard, HF Willard, HF Willard, HM Rosenberg, Ian Dunham, J Anastasi, J Meyne, JE Koch, JS Waye, JS Waye, JS Waye, L Manuelidis, L Manuelidis, M Klever, P Emmerich, PJ Poddighe, PR Langer, S Popp, T Cremer, T Cremer, T Cremer, T Imai, T Maniatis, Terry Featherstone, Thomas Cremer   +33 more
core   +1 more source

DNA-directed termination of mammalian RNA polymerase II

Genes & Development
ABSTRACTThe best-studied mechanism of eukaryotic RNA polymerase II (RNAPII) transcriptional termination involves polyadenylation site-directed cleavage of the nascent RNA. The RNAPII-associated cleavage product is then degraded by XRN2, dislodging RNAPII from the DNA template.
Davidson, Lee, Rouvière, Jérôme O., Sousa-Luís, Rui, Nojima, Takayuki, Proudfoot, Nicholas J., Jensen, Torben Heick, West, Steven   +6 more
openaire   +3 more sources

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

FANCM promotes PARP inhibitor resistance by minimizing ssDNA gap formation and counteracting resection inhibition

Cell Reports
Summary: Poly(ADP-ribose) polymerase inhibitors (PARPis) exhibit remarkable anticancer activity in tumors with homologous recombination (HR) gene mutations. However, the role of other DNA repair proteins in PARPi-induced lethality remains elusive.
Zeyuan Liu, Huadong Jiang, Sze Yuen Lee, Nannan Kong, Ying Wai Chan   +4 more
doaj   +1 more source

Essential and non-essential DNA replication genes in the model halophilic Archaeon, Halobacterium sp. NRC-1

BMC Genetics, 2007
Background Information transfer systems in Archaea, including many components of the DNA replication machinery, are similar to those found in eukaryotes.
DasSarma Shiladitya, DasSarma Priya, Berquist Brian R   +2 more
doaj   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Low Cost DNA Molecular Weight Marker: Primer-Directed Synthesis from pGEM-T Easy Vector

Walailak Journal of Science and Technology, 2011
A low cost DNA molecular weight marker was produced by a marker primer-directed synthetic method using pGEM-T Easy vector as the DNA template. Seven primers were used to amplify eight different DNA fragments, which were 150, 300, 375, 500, 700, 1,000, 1 ...
Siriporn RIYAJAN, Wiparat YENTUA, Jaros PHINCHONGSAKULDIT   +2 more
doaj   +1 more source

Translesion synthesis in mammalian cells [PDF]

, 2006
DNA damage blocks the progression of the replication fork. In order to circumvent the damaged bases, cells employ specialized low stringency DNA polymerases, which are able to carry out translesion synthesis (TLS) past different types of damage. The five
Alan R. Lehmann, Avkin, Avkin, Bienko, Garg, Gibbs, Gibbs, Gibbs, Guo, Haracska, Hoege, Huang, Johnson, Kannouche, Kannouche, King, Lehmann, Lemontt, Ling, Maher, Masutani, Masutani, McCulloch, Murakumo, Nair, Nakajima, Nelson, Nelson, Nelson, Ogi, Ohmori, Pages, Stelter, Tissier, Vidal, Watanabe   +35 more
core   +2 more sources

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source