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The DNA-directed RNA polymerases of soybean
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973Abstract Four cellular RNA polymerases have been partially purified from hypocotyls of soybean ( Glycine max ). Enzyme isolation from nuclear preparations indicate the DEAE polymerases I and II are localized within the nucleus. Inhibitor studies showed that DEAE polymerase II was preferentially inhibited by α-amanitin, DEAE peaks III and IV were ...
Joe L. Key, Paul A. Horgen
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Effect of granaticin on the activity of DNA-directed RNA polymerase fromStreptomyces granaticolor
Folia Microbiologica, 1989DNA-directed RNA polymerase was isolated from wild-type and mutant (asporogenic and granaticin overproducing) Streptomyces granaticolor. The effect of granaticin on the enzyme activity was compared with that of standard transcription inhibitors, rifampicin and rifamycin SV and shown to be zero.
Spízek J +3 more
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DNA-directed RNA polymerase from HeLa cells
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975Abstract DNA-directed RNA polymerase was solubilized from total HeLa cells. Three distinct classes of the enzyme could be clearly differentiated by their sensitivity toward α-amanitin. While form A is completely resistant to high concentrations (133 μg/ml) of this toxin, enzyme B is highly sensitive and is completely inhibited by concentrations of 0 ...
Bernd J. Benecke, Klaus H. Seifart
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The isolation and properties of a DNA-directed RNA polymerase from yeast mitochondria
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976A method is described for the rapid isolation of yeast mitochondrial DNA-directed RNA polymerase. The enzyme obtained had a specific activity of 1.56 nmol UMP incorporated per mg protein in 20 min at 37 degrees C, and is some 95% pure. This purified enzyme upon polyacrylamide gel elctrophoresis consists of a single polypeptide of 68 000 mol.
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Journal of Cancer Research and Clinical Oncology, 2023
DNA-directed RNA polymerase (DDRP) related genes and long non-coding RNAs (lncRNAs) play an important role in the development of lung adenocarcinoma (LUAD), the leading cause of cancer-related death worldwide. Therefore, we aimed to construct a DDRP-associated lncRNA model to predict the prognosis of LUAD and to evaluate its sensitivity to ...
Jiaao, Yu +3 more
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DNA-directed RNA polymerase (DDRP) related genes and long non-coding RNAs (lncRNAs) play an important role in the development of lung adenocarcinoma (LUAD), the leading cause of cancer-related death worldwide. Therefore, we aimed to construct a DDRP-associated lncRNA model to predict the prognosis of LUAD and to evaluate its sensitivity to ...
Jiaao, Yu +3 more
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Mitochondrial DNA-directed RNA polymerase from Saccharomyces cerevisae mitochondria
Biochemical and Biophysical Research Communications, 1971Abstract A DNA-directed RNA polymerase activity has been detected in yeast mitochondrial extracts which is sensitive to rifampicin. This activity is distinct from that found in the nucleus and cytoplasm, and is possibly coded for, or under the control of the mitochondrial genome.
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Environmental Toxicology and Pharmacology, 2012
The aryl hydrocarbon receptor (AhR) mediates a variety of biological responses to ubiquitous environmental pollutants. AhR is ligand activated transcription factor with high affinities for aromatic planar compounds such as β-naphthoflavone (BNF), 3-methylcholanthrene (3-MC), benzo[a]pyrene (BaP) or dioxin (TCDD).
Agnieszka Anna Rawłuszko, Damian Brauze
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The aryl hydrocarbon receptor (AhR) mediates a variety of biological responses to ubiquitous environmental pollutants. AhR is ligand activated transcription factor with high affinities for aromatic planar compounds such as β-naphthoflavone (BNF), 3-methylcholanthrene (3-MC), benzo[a]pyrene (BaP) or dioxin (TCDD).
Agnieszka Anna Rawłuszko, Damian Brauze
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Journal of Proteome Research, 2014
Enzymatic machineries fundamental for information processing (e.g., transcription, replication, translation) in Archaea are simplified versions of their eukaryotic counterparts. This is clearly noticeable in the conservation of sequence and structure of corresponding enzymes (see for example the archaeal DNA-directed RNA polymerase (RNAP)). In Eukarya,
Mikel Azkargorta +3 more
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Enzymatic machineries fundamental for information processing (e.g., transcription, replication, translation) in Archaea are simplified versions of their eukaryotic counterparts. This is clearly noticeable in the conservation of sequence and structure of corresponding enzymes (see for example the archaeal DNA-directed RNA polymerase (RNAP)). In Eukarya,
Mikel Azkargorta +3 more
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Biological Chemistry Hoppe-Seyler, 1989
Stimulation of growth in resting cultures of the ciliated protozoan Tetrahymena thermophila stimulated putrescine formation in a manner coordinated to transcription and partly to DNA-polymerisation. The stimulation of polyamine biosynthesis involved increased formation of the precursor L-ornithine from L-arginine as well as stimulation of the ...
Wolfgang Eichler, Reinhard Corr
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Stimulation of growth in resting cultures of the ciliated protozoan Tetrahymena thermophila stimulated putrescine formation in a manner coordinated to transcription and partly to DNA-polymerisation. The stimulation of polyamine biosynthesis involved increased formation of the precursor L-ornithine from L-arginine as well as stimulation of the ...
Wolfgang Eichler, Reinhard Corr
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