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Key Players in the Methanogenic Biodegradation of n-Hexadecane Identified by DNA-Stable Isotope Probing

International Biodeterioration and Biodegradation, 2019
Abstract Methanogenic biodegradation of n-alkanes is an important process in the biogeochemical carbon cycles. The key players in this biochemical processes were investigated for a 920-day of culturing with universally labelled (UL)-13C-hexadecane and 1, 2-13C-hexadecane as the sources of carbon and energy, using stable isotope probing combined with ...
Jin-Feng Liu, Bo-Zhong Mu, Ji-Dong Gu
exaly   +2 more sources

Identification of metolachlor mineralizing bacteria in aerobic and anaerobic soils using DNA-stable isotope probing

Biodegradation, 2017
The influence of soil environmental factors such as aeration on the ecology of microorganisms involved in the mineralization and degradation of the popular soil-applied pre-emergent herbicide, metolachlor is unknown. To address this knowledge gap, we utilized DNA-based stable isotope probing (SIP) where soil microcosms were incubated aerobically or ...
Ramdas G, Kanissery   +4 more
openaire   +2 more sources

Use of DNA‐stable isotope probing and functional gene probes to investigate the diversity of methyl chloride‐utilizing bacteria in soil

Environmental Microbiology, 2005
Summary Enrichment and isolation of methyl chloride‐utilizing bacteria from various terrestrial environments, including woodland and forest soils, resulted in the identification of seven methyl chloride‐utilizing strains belonging to the genus Hyphomicrobium , an
Elena, Borodina   +3 more
openaire   +2 more sources

Unveiling plastic biodegradation pathways through 13C-DNA stable isotope probing and metagenomics

Journal of Hazardous Materials
Polyhydroxyalkanoates (PHAs) are promising biobased and biodegradable alternatives to conventional plastics, yet their degradation mechanisms and the diversity of microorganisms involved remain poorly characterized in marine ecosystems. Here, we used 13C-labeled poly(3-hydroxybutyrate) (PHB) and combined DNA-stable isotope probing (DNA-SIP) with ...
Barbe, Valérie   +14 more
openaire   +2 more sources

Cross‐feeding of methane carbon among bacteria on rice roots revealed by DNA‐stable isotope probing

Environmental Microbiology Reports, 2009
Summary Most of methane in flooded rice fields is emitted via transport through the plant gas vascular system. In the reverse direction, oxygen is diffusing to the living roots, and hence, the rhizosphere and roots of rice serve as an important habitat for CH 4 oxidation ...
Qiu, Q., Conrad, R., Lu, Y.
openaire   +3 more sources

[Applications and perspectives of DNA stable-isotope probing in metagenomics: a review].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2013
DNA stable-isotope probing (DNA-SIP) is a recently developed method with which the incorporation of stable isotope from a labeled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic ...
Wei, Liu   +3 more
openaire   +1 more source

Identification of novel potential acetate-oxidizing bacteria in thermophilic methanogenic chemostats by DNA stable isotope probing

Applied Microbiology and Biotechnology, 2019
Syntrophic oxidization of acetate and propionate are both critical steps of methanogenesis during thermophilic anaerobic digestion. However, knowledge on syntrophic acetate-oxidizing bacteria (SAOB) and syntrophic propionate-oxidizing bacteria (SPOB) is limited because of the difficulty in pure culture isolation due to symbiotic relationship.
Dan Zheng   +7 more
openaire   +2 more sources

Mechanism of microplastics promoting sulfamethoxazole biodegradation in activated sludge as revealed by DNA-stable isotope probing

Science of The Total Environment
Microplastics (MPs) often coexist with sulfonamide antibiotics (SAs) in the activated sludge of wastewater treatment plants (WWTPs). Microbial degradation is a crucial pathway for SAs removal in the activated sludge, though its response to MPs still yet to be disclosed. Here, we combined DNA-stable isotope probing (DNA-SIP), PICRUSt and MENA techniques
Xueqing, Wu   +6 more
openaire   +2 more sources

ออโตโทรฟิคแอมโมเนียออกซิเดชันของแอมโมเนียออกซิไดซิงอาร์เคียและแบคทีเรียในระบบบำบัดน้ำเสียด้วยเทคนิค DNA stable isotope probing (DNA-SIP)

การกำจัดแอมโมเนียในระบบบำบัดน้ำเสียในปัจจุบันเป็นการทำงานร่วมกันระหว่างจุลินทรีย์สองกลุ่ม คือ แอมโมเนียออกซิไดซิงอาร์เคีย (Ammonia-oxidizing archaea, AOA) และแอมโมเนียออกซิไดซิงแบคทีเรีย (Ammonia-Oxidizing bacteria, AOB) จากการศึกษากิจกรรมของจุลินทรีย์กลุ่ม AOB ที่ผ่านมาขณะออกซิไดซ์แอมโมเนียพบว่ามีการทำงานแบบออโตโทรป (Autotroph) คือใช้แหล่งคาร ...
openaire   +1 more source

Root exudate-consuming microbial community structure by DNA stable isotope probing

2006
International ...
Balesdent, Jérôme   +4 more
openaire   +1 more source

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