Results 251 to 260 of about 5,185,708 (294)
Some of the next articles are maybe not open access.
Biochemistry, 2000
Twelve classes of deoxyribozymes that promote an ATP-dependent "self-capping" reaction were isolated by in vitro selection from a random-sequence pool of DNA. Each deoxyribozyme catalyzes the transfer of the AMP moiety of ATP to its 5'-terminal phosphate group, thereby forming a 5',5'-pyrophosphate linkage.
Y, Li, Y, Liu, R R, Breaker
openaire +2 more sources
Twelve classes of deoxyribozymes that promote an ATP-dependent "self-capping" reaction were isolated by in vitro selection from a random-sequence pool of DNA. Each deoxyribozyme catalyzes the transfer of the AMP moiety of ATP to its 5'-terminal phosphate group, thereby forming a 5',5'-pyrophosphate linkage.
Y, Li, Y, Liu, R R, Breaker
openaire +2 more sources
Current Protocols in Molecular Biology, 2008
AbstractThis unit presents characteristics and reaction conditions of the DNA‐dependent DNA polymerases, including E. coli DNA polymerase I and its Klenow fragment, T4 DNA polymerase, native and modified T7 DNA polymerase, phi29 DNA polymerase, Bst DNA polymerase, and Taq DNA polymerase. The unit also provides overviews of other classes of thermophilic
Rebecca B, Kucera, Nicole M, Nichols
openaire +2 more sources
AbstractThis unit presents characteristics and reaction conditions of the DNA‐dependent DNA polymerases, including E. coli DNA polymerase I and its Klenow fragment, T4 DNA polymerase, native and modified T7 DNA polymerase, phi29 DNA polymerase, Bst DNA polymerase, and Taq DNA polymerase. The unit also provides overviews of other classes of thermophilic
Rebecca B, Kucera, Nicole M, Nichols
openaire +2 more sources
Journal of the American Chemical Society, 2004
Cloning DNA typically involves the joining of target DNAs with vector constructs by enzymatic ligation. A commonly used enzyme for this reaction is bacteriophage T4 DNA ligase, which requires ATP as the energy source to catalyze the otherwise unfavorable formation of a phosphodiester bond.
Alavattam, Sreedhara +2 more
openaire +2 more sources
Cloning DNA typically involves the joining of target DNAs with vector constructs by enzymatic ligation. A commonly used enzyme for this reaction is bacteriophage T4 DNA ligase, which requires ATP as the energy source to catalyze the otherwise unfavorable formation of a phosphodiester bond.
Alavattam, Sreedhara +2 more
openaire +2 more sources
Active DNA demethylation damages DNA
Science, 2022Active DNA demethylation maintains enhancer activity in nonproliferating cells but can damage ...
Isaac F, López-Moyado, Anjana, Rao
openaire +2 more sources
DNA Elasticity from Short DNA to Nucleosomal DNA
The Journal of Physical Chemistry B, 2015Active biological processes like transcription, replication, recombination, DNA repair, and DNA packaging encounter bent DNA. Machineries associated with these processes interact with the DNA at short length (
Garai, Ashok +3 more
openaire +3 more sources
Forensic Science, Medicine, and Pathology, 2007
The rapid development, success, and occasional failures of forensic DNA profiling are highly publicised, and as a consequence are well known to the scientific and public communities alike. Over the same period of time that forensic DNA typing has accelerated onto the scene, another related discipline has been born and has made equally, or perhaps, even
openaire +2 more sources
The rapid development, success, and occasional failures of forensic DNA profiling are highly publicised, and as a consequence are well known to the scientific and public communities alike. Over the same period of time that forensic DNA typing has accelerated onto the scene, another related discipline has been born and has made equally, or perhaps, even
openaire +2 more sources
EcoRI DNA methyltransferase-DNA interactions
Biochemistry, 1992We present a novel strategy with synthetic hemimethylated DNA substrates containing uracil for thymine and inosine for guanosine replacements and EcoRI DNA methyltransferase to characterize the importance of major groove hydrophobic groups to the sequence-specific modification of DNA.
N O, Reich, M J, Danzitz
openaire +2 more sources
Annual Review of Biochemistry, 1981
The various problems of disentangling DNA strands or duplexes in a cell are all rooted in the double-helical structure of DNA. Three distinct subfamilies of enzymes, known as the DNA topoisomerases, have evolved to solve these problems. This review focuses on work in the past decade on the mechanisms and cellular functions of these enzymes.
openaire +4 more sources
The various problems of disentangling DNA strands or duplexes in a cell are all rooted in the double-helical structure of DNA. Three distinct subfamilies of enzymes, known as the DNA topoisomerases, have evolved to solve these problems. This review focuses on work in the past decade on the mechanisms and cellular functions of these enzymes.
openaire +4 more sources
Science, 1988
DNA looping is widely used in nature. It is well documented in the regulation of prokaryotic and eukaryotic gene expression, DNA replication, and site-specific DNA recombination. Undoubtedly looping also functions in other protein-DNA transactions such as repair and chromosome segregation.
openaire +3 more sources
DNA looping is widely used in nature. It is well documented in the regulation of prokaryotic and eukaryotic gene expression, DNA replication, and site-specific DNA recombination. Undoubtedly looping also functions in other protein-DNA transactions such as repair and chromosome segregation.
openaire +3 more sources
DNA topoisomerases and DNA repair
BioEssays, 1988AbstractDNA topoisomerases are enzymes that can modify, and may regulate, the topological state of DNA through concerted breaking and rejoining of the DNA strands. They have been believed to be directly involved in DNA excision repair, and perhaps to be required for the control of repair as well. The vicissitudes of this hypothesis provide a noteworthy
C S, Downes, R T, Johnson
openaire +2 more sources