Results 81 to 90 of about 6,778,475 (339)

Dynamics of Bacteriophage Genome Ejection In Vitro and In Vivo

, 2010
Bacteriophages, phages for short, are viruses of bacteria. The majority of phages contain a double-stranded DNA genome packaged in a capsid at a density of ~500 mg/ml. This high density requires substantial compression of the normal B form helix, leading
Molineux, Ian J., Panja, Debabrata
core   +1 more source

Helical Chirality: a Link between Local Interactions and Global Topology in DNA [PDF]

, 2010
DNA supercoiling plays a major role in many cellular functions. The global DNA conformation is however intimately linked to local DNA-DNA interactions influencing both the physical properties and the biological functions of the supercoiled molecule ...
A Minsky, A Stasiak, A Travers, AA Kornyshev, AA Travers, AA Vetcher, AK McClendon, AV Vologodskii, AV Vologodskii, BF Eichman, C Brochier-Armanet, CJ Benham, DE Pulleyblank, DI Cherny, DM Lilley, E Marguet, EL Zechiedrich, F Charbonnier, FH Crick, G Charvin, H Wong, HM Berman, IG Panyutin, J Yan, JC Wang, JF Marko, KC Dong, KC Neuman, KD Corbett, KD Corbett, L Postow, LF Liu, LS Shlyakhtenko, LS Shlyakhtenko, M Graille, M Kampmann, MD Stone, N Nöllmann, NJ Crisona, O Espeli, O Guipaud, P Forterre, P Forterre, P Lopez-Garcia, P Lopez-Garcia, P Várnai, PJJ Robinson, Péter Várnai, R Kanaar, R Lavery, S Inoue, S Shaw, S Trigueros, SS Zakharova, Stefan Wölfl, T Bankhead, T Schalch, T Schlick, T Stuchinskaya, TR Strick, V Katrich, V Stupina, VV Rybenkov, W Humphrey, WK Olson, WL DeLano, X Qiu, Y Timsit, Y Timsit, Y Timsit, Y Timsit, Y Timsit, Y Timsit, Y-C Xu, Youri Timsit, Z Liu, ZJ Tan   +76 more
core   +3 more sources

DNA Polymerase-Catalyzed DNA Network Growth [PDF]

Journal of the American Chemical Society, 2008
The distinct base pairing property of DNA is an advantageous phenomenon that has been exploited in the usage of DNA as scaffold for directed self-organization to form nanometer-sized objects in a desirable fashion. Herein we report the construction of three-dimensional DNA-based networks that can be generated and amplified by the DNA polymerase chain ...
Keller, Sascha, Marx, Andreas, Wang, Jie, Chandra, Madhaviah, Berger, Rüdiger   +4 more
openaire   +4 more sources

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

PARP-3 and APLF function together to accelerate nonhomologous end joining [PDF]

, 2010
PARP-3 is a member of the ADP-ribosyl transferase superfamily of unknown function. We show that PARP-3 is stimulated by DNA double-strand breaks (DSBs) in vitro and functions in the same pathway as the poly (ADP-ribose)-binding protein APLF to accelerate
Ahel, Ame, Anna E.O. Fisher, Audebert, Bekker-Jensen, Benjamin, Bernardo Reina-San-Martin, Boulton, Brown, Bryant, Bryant, Caldecott, Caldecott, Critchlow, Ding, Drouet, El-Khamisy, Farmer, Fisher, Grawunder, Guy Poirier, Hochegger, Hottiger, Iles, Isabelle Robert, Kanno, Keith W. Caldecott, Kickhoefer, Kleine, Le Page, Lehtio, Limei Ju, Lobrich, Loseva, Macrae, Mahaney, Maria C. Zuma, Mehrotra, Michele Rouleau, Morrison, Okano, Otto, Poirier, Robert, Roberts, Rogakou, Rogakou, Rouleau, Rulten, Smith, Soulas-Sprauel, Stevnsner, Stuart L. Rulten, Sugimura, Trucco, Wang, Yan, Yang, Yano, Yano   +59 more
core   +1 more source

Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancer

FEBS Letters, EarlyView.
Anaplastic thyroid cancer (ATC) lacks iodide uptake ability due to MAPK activation increasing the expression of the histone methyltransferase EZH2, which represses thyroid differentiation genes (TDGs) such as the sodium iodide symporter (NIS). Dual inhibition of MAPK (U0126) and EZH2 (EPZ6438/Tazemetostat) reverses this mechanism, thus restoring TDG ...
Diego Claro de Mello, Marcella Maringolo Cristovão, Guilherme Henrique, Vinicius Gonçalves Rodrigues, Caroline Serrano‐Nascimento, Edna Teruko Kimura, Cesar Seigi Fuziwara   +6 more
wiley   +1 more source

Method for systematic targeted isolation of homologous cDNA fragments in a multiplex format

BioTechniques, 2004
In this study, a two-step method for systematic multiplex cloning of homologous cDNAs from related species was developed. The first step, called MUCH (multiplex cloning of homologous genes), is cloning of partial but authentic cDNA fragments of ...
Reiko Ohara, Hisashi Koga, Reiko Kikuno, Osamu Ohara   +3 more
doaj   +1 more source

Defects Can Increase the Melting Temperature of DNA-Nanoparticle Assemblies

, 2007
DNA-gold nanoparticle assemblies have shown promise as an alternative technology to DNA microarrays for DNA detection and RNA profiling. Understanding the effect of DNA sequences on the melting temperature of the system is central to developing reliable ...
Harris, Nolan C., Kiang, Ching-Hwa
core   +1 more source

Hematopoietic (stem) cells—The elixir of life?

FEBS Letters, EarlyView.
The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo, Jonah Anderson, Emilie Dinh Vedrenne, Noël Yeh Martín, Jette Lengefeld   +4 more
wiley   +1 more source

Multiplexed DNA-Modified Electrodes [PDF]

, 2010
We report the use of silicon chips with 16 DNA-modified electrodes (DME chips) utilizing DNA-mediated charge transport for multiplexed detection of DNA and DNA-binding protein targets.
Barton, Jacqueline K., Gorodetsky, Alon A., Muren, Natalie B., Slinker, Jason D.   +3 more
core   +2 more sources