Results 181 to 190 of about 2,473,744 (242)

Thermal Bed Design for Temperature-Controlled DNA Amplification Using Optoelectronic Sensors. [PDF]

Sensors (Basel)
Garcia-Torales G, Torres-Ortega HH, Estrada-Marmolejo R, Beltran-Gonzalez AB, Strojnik M.   +4 more
europepmc   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

Exploring Life Detection on Mars: Understanding Challenges in DNA Amplification in Martian Regolith Analogue After Fe Ion Irradiation. [PDF]

Life (Basel)
Cassaro A, Pacelli C, Onofri S.
europepmc   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Characterization and integrated analysis of extrachromosomal DNA amplification in hematological malignancies. [PDF]

Neoplasia
Zhang H, Liu B, Cheng J, Li Z, Jia M, Li M, Zhao L, Wang L, Xi Y.   +8 more
europepmc   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Effects of fixation and demineralization on histomorphology and DNA amplification of canine bone marrow. [PDF]

Vet Pathol
Diamantino GML, Beeler-Marfisi J, Foster RA, Sears W, Defarges A, Vernau W, Bienzle D.   +6 more
europepmc   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Protocol for genome-wide analysis of somatic variants at single-cell resolution using primary template-directed DNA amplification. [PDF]

STAR Protoc
Derks LLM, van Leeuwen AJCN, Steemers AS, Trabut L, van Roosmalen MJ, Poort VM, Hagelaar R, Verheul M, Middelkamp S, van Boxtel R.   +9 more
europepmc   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source