Results 81 to 90 of about 2,473,744 (242)

Development and validation of a T7 based linear amplification for genomic DNA

BMC Genomics, 2003
Background Genomic maps of transcription factor binding sites and histone modification patterns provide unique insight into the nature of gene regulatory networks and chromatin structure.
Schreiber Stuart L, Liu Chih Long, Bernstein Bradley E   +2 more
doaj   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Truncated Amplification: A Method for High-Fidelity Template-Driven Nucleic Acid Amplification

BioTechniques, 2002
The error rate of conventional PCR is problematic when amplifying from single cells or amplifying segments for protein functional analysis by in vitro translation. We describe truncated amplification, a method for high-fidelity amplification in which DNA
Qiang Liu, Piotr Swiderski, Steve S. Sommer   +2 more
doaj   +1 more source

Whole genome amplification for PGD and PND; molecular and a-CGH diagnosis [PDF]

, 2009
Whole genome amplification amplifies the entire genome in a few hours from samples of minimal DNA quantities, even from single cells. This may have many applications, especially in prenatal diagnosis, PGD and PGS. The hypothesis for chapter 3 was: Can
Glentis, S.
core  

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification

BioTechniques, 2014
The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used.
Konstantin B. Ignatov, Ekaterina V. Barsova, Arkady F. Fradkov, Konstantin A. Blagodatskikh, Tatiana V. Kramarova, Vladimir M. Kramarov   +5 more
doaj   +1 more source

Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies. [PDF]

, 2008
BACKGROUND: The amplification of bacterial RNA is required if complex host-pathogen interactions are to be studied where the recovery of bacterial RNA is limited.
Laing Ken, Waddell, SJ, Butcher, PD, Senner Claire, Simon Waddell (4462816), Ken Laing, Laing, K, Ken Laing (173955), Philip D Butcher, Waddell Simon J, Butcher Philip D, Claire Senner (44405), Simon J Waddell, Claire Senner, Senner, C, Philip D Butcher (16047203)   +15 more
core   +1 more source

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

BMC Biotechnology, 2011
Background Quantitative real-time PCR (qPCR) is becoming increasingly important for DNA genotyping and gene expression analysis. For continuous monitoring of the production of PCR amplicons DNA-intercalating dyes are widely used.
Dráberová Lubica, Bugajev Viktor, Eitler Jiří, Shaik Gouse M, Polakovičová Iva, Horáková Helena, Dráber Petr   +6 more
doaj   +1 more source

Analysis of the giant genomes of Fritillaria (Liliaceae) indicates that a lack of DNA removal characterizes extreme expansions in genome size. [PDF]

, 2015
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.Plants exhibit an extraordinary range of genome sizes,
Novák, P, Pellicer, J., Fay, M. F., Leitch, IJ, Neumann, P., Peter D. Day, Leitch, A. R., Pellicer, J, Michael F. Fay, Petr Novák, Ilia J. Leitch, Day, PD, Kelly, L. J., Richard A. Nichols, Pavel Neumann, Lysak, M. A., Laura J. Kelly, Neumann, P, Jaume Pellicer, Nichols, RA, Andrew R. Leitch, Macas, J., Macas, J, Novak, P., Leitch, I. J., Lysak, M., Berger, M., Lysak, MA, Day, P. D., Martin A. Lysak, Renny-Byfield, S, Leitch, AR, Nichols, R. A., Jiří Macas, Simon Renny‐Byfield, Kelly, LJ, Berger, M, Fay, MF, Madeleine Berger, Renny-Byfield, S.   +39 more
core   +1 more source