Integrated one‐pot CRISPR‐Cas detection: Addressing strategies and future prospects
Integrated one‐pot CRISPR‐Cas detection significantly simplifies workflows, reduces cross‐contamination risks, and shortens turnaround times, making it highly suitable for point‐of‐care testing (POCT) and resource‐limited settings. Key challenges include system compatibility issues between Cas proteins and isothermal amplification, limited multiplexing
Jian Zhou +6 more
wiley +1 more source
Thoracic aortic aneurysm (TAA) is a life‐threatening cardiovascular disease with limited therapeutic options. Through single‐cell RNA sequencing of aortic tissues from healthy individuals and TAA patients (105,541 cells), we identified CLEC5A+macrophages as the predominant pathogenic cell population exhibiting the highest M1 polarization score. Machine
Xiangyu Li +3 more
wiley +1 more source
Advancing Yeast Identification Using High-Throughput DNA Barcode Data From a Curated Culture Collection. [PDF]
Vu D +12 more
europepmc +1 more source
Elevated Hemoglobin A2: A Molecular Revisited, and Implications to β‐Thalassemia Screening
In Thailand, the Hb A2 cut‐off value for β‐thalassemia carrier has been changed from 4.0% to 3.6% since 2015. We examined the molecular basis of β‐thalassemia in a large cohort of Thai subjects with this change. The molecular basis of β‐thalassemia was updated, and a change in the Hb A2 cut‐off can alter this spectrum.
Kritsada Singha +8 more
wiley +1 more source
A DNA Barcode Inventory of Austrian Dragonfly and Damselfly (Insecta: Odonata) Species. [PDF]
Zangl L +16 more
europepmc +1 more source
DNA barcoding for identification of species involved in wildlife strikes at Brazilian airports
A collaborative network of 42 Brazilian airports applied DNA barcoding to identify 80 species involved in wildlife strikes. Among them, 32 species (17 birds and 15 bats) were not currently available in Brazil's wildlife strike reporting database, underscoring the value of molecular tools for strengthening wildlife strike monitoring. Abstract This study
Andre Akira Gonzaga Yoshikawa +11 more
wiley +1 more source
A new species of <i>Athelges</i> (Crustacea, Isopoda, Epicaridea, Bopyridae) from Japan, with morphological and DNA barcode data. [PDF]
Shinoda H +4 more
europepmc +1 more source
ABSTRACT Since 2019, coronavirus disease 2019 (COVID‐19) has been associated with increased risks of preterm birth and placental complications. We prospectively investigated alterations in microRNAs (miRNAs) and cytokines in placental and amniotic tissues from pregnant women with and without COVID‐19 to evaluate the infection's impact on pregnancy ...
Wei‐Chun Chen +3 more
wiley +1 more source
Well-Known, Misidentified, or Unnamed? A DNA Barcode-Based Reassessment of the Lepidoptera Fauna of Cyprus Supported by Morphology. [PDF]
Huemer P +7 more
europepmc +1 more source
Effect of fuchsin fixation of pollen on DNA barcode recovery. [PDF]
Streicher MB +2 more
europepmc +1 more source

