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DNA Barcoding of Marine Metazoans
2016The accumulation of DNA barcode sequences will provide an increasingly useful and comprehensive library for species identification and discovery of marine metazoans. Here we present a summary of protocols designed to obtain DNA barcodes of marine metazoans from diverse phyla.
Dirk, Steinke +2 more
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DNA barcodes of Philippine accipitrids
Molecular Ecology Resources, 2010AbstractDNA barcoding is a molecular method that rapidly identifies an individual to a known taxon or its closest relative based on a 650‐bp fragment of the cytochrome c oxidase subunit I (COI). In this study, DNA barcodes of members of the family Accipitridae, including Haliastur indus (brahminy kite), Haliaeetus leucogaster (white‐bellied sea eagle),
Perry S, Ong +6 more
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DNA Barcoding Methods for Invertebrates
2012Invertebrates comprise approximately 34 phyla, while vertebrates represent one subphylum and insects a (very large) class. Thus, the clades excepting vertebrates and insects encompass almost all of animal diversity. Consequently, the barcoding challenge in invertebrates is that of barcoding animals in general.
Nathaniel, Evans, Gustav, Paulay
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DNA Barcoding of Marine Metazoa
Annual Review of Marine Science, 2011More than 230,000 known species representing 31 metazoan phyla populate the world's oceans. Perhaps another 1,000,000 or more species remain to be discovered. There is reason for concern that species extinctions may outpace discovery, especially in diverse and endangered marine habitats such as coral reefs.
Ann, Bucklin +2 more
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Generating 2D Barcode for DNA Barcode Sequences
DNA barcode sequence is a short DNA sequence representing a sample from a particular species. The commonly used DNA barcodes are at least 200 bps long. This large number of characters cannot be encoded in two-dimensional codes for sample recognition and tracking. In the present study, we described a method that can be used to compress the DNA sequencesRui, Liu +3 more
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DNA Barcodes: Methods and Protocols
2012DNA barcoding, a new method for the quick identification of any species based on extracting a DNA sequence from a tiny tissue sample of any organism, is now being applied to taxa across the tree of life. As a research tool for taxonomists, DNA barcoding assists in identification by expanding the ability to diagnose species by including all life history
W John, Kress, David L, Erickson
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Methods for DNA Barcoding of Fungi
2012This chapter describes methods currently used for DNA barcoding of fungi, including some comments on the barcoding of aged herbarium material. The collecting procedures are focussed on macro-fungi. The laboratory methods are for medium-throughput DNA barcoding, targeted at the 96-well format, but without the assistance of robotics. In the absence of an
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ddRAD Sequencing and DNA Barcoding
Double-digest restriction site-associated DNA sequencing is a library preparation protocol that enables capturing variable sites across the genome including single-nucleotide polymorphisms (SNPs). These SNPs can be utilized to gain evolutionary insights into patterns observed in DNA barcodes, to infer population structure and phylogenies, to detect ...Mutanen Marko +2 more
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