Results 311 to 320 of about 90,456 (335)
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Acta Tropica, 2012
Due to the difficulty in morphological identification the development of reliable molecular tools for species distinction is a priority for piroplasma. Previous studies based on 18S rRNA and other gene sequences provided a backbone for the phylogeny of piroplasma. However, it is difficult to discriminate species in a comprehensive sample.
Qiaoyun Ren +11 more
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Due to the difficulty in morphological identification the development of reliable molecular tools for species distinction is a priority for piroplasma. Previous studies based on 18S rRNA and other gene sequences provided a backbone for the phylogeny of piroplasma. However, it is difficult to discriminate species in a comprehensive sample.
Qiaoyun Ren +11 more
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2012
This chapter is an overview of the techniques for DNA barcoding of fishes from field collection to DNA sequence analysis. Recommendations for modifications of field protocols and best tissue sampling practices are made. A variety of DNA extraction protocols is provided, including high-throughput robot-assisted methods. A pair of well-tested forward and
Carole C. Baldwin +3 more
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This chapter is an overview of the techniques for DNA barcoding of fishes from field collection to DNA sequence analysis. Recommendations for modifications of field protocols and best tissue sampling practices are made. A variety of DNA extraction protocols is provided, including high-throughput robot-assisted methods. A pair of well-tested forward and
Carole C. Baldwin +3 more
openaire +2 more sources
Russian Journal of Genetics, 2021
The method of DNA barcoding has become a reliable tool that allows fast and accurate identification and verifies biodiversity in the hands of an experienced taxonomist. This method is very popular in various fields of human activity because of its ease of use and economic benefit.
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The method of DNA barcoding has become a reliable tool that allows fast and accurate identification and verifies biodiversity in the hands of an experienced taxonomist. This method is very popular in various fields of human activity because of its ease of use and economic benefit.
openaire +2 more sources
2012
Conventional DNA barcoding uses an approximately 650 bp DNA barcode of the mitochondrial gene COI for species identification in animal groups. Similar size fragments from chloroplast genes have been proposed as barcode markers for plants. While PCR amplification and sequencing of a 650 bp fragment is consistent in freshly collected and well-preserved ...
Charly McKenna, Mehrdad Hajibabaei
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Conventional DNA barcoding uses an approximately 650 bp DNA barcode of the mitochondrial gene COI for species identification in animal groups. Similar size fragments from chloroplast genes have been proposed as barcode markers for plants. While PCR amplification and sequencing of a 650 bp fragment is consistent in freshly collected and well-preserved ...
Charly McKenna, Mehrdad Hajibabaei
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2020
DNA barcoding is a very useful and promising approach to the identification of biological species in which DNA sequences from a tissue sample of the target organism serve as the basic key for information retrieval. Old existing software for evolutionary analysis of DNA (specific or genomic) sequences was developed for taxonomic and phylogenetic ...
Ena Ray Banerjee, Payal Pal
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DNA barcoding is a very useful and promising approach to the identification of biological species in which DNA sequences from a tissue sample of the target organism serve as the basic key for information retrieval. Old existing software for evolutionary analysis of DNA (specific or genomic) sequences was developed for taxonomic and phylogenetic ...
Ena Ray Banerjee, Payal Pal
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Biology Bulletin Reviews, 2019
Abstract—The search for universal plant DNA barcodes has proved to be a big challenge. A single locus (or combination of several loci) that can be used for species identification has yet to be revealed, though the (rbcL + matK+ ITS) combination recommended in 2009 as the standard makes it possible to assign a species to the corresponding genus.
Alexander V. Rodionov +2 more
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Abstract—The search for universal plant DNA barcodes has proved to be a big challenge. A single locus (or combination of several loci) that can be used for species identification has yet to be revealed, though the (rbcL + matK+ ITS) combination recommended in 2009 as the standard makes it possible to assign a species to the corresponding genus.
Alexander V. Rodionov +2 more
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DNA barcodes of Philippine accipitrids
Molecular Ecology Resources, 2010AbstractDNA barcoding is a molecular method that rapidly identifies an individual to a known taxon or its closest relative based on a 650‐bp fragment of the cytochrome c oxidase subunit I (COI). In this study, DNA barcodes of members of the family Accipitridae, including Haliastur indus (brahminy kite), Haliaeetus leucogaster (white‐bellied sea eagle),
Anna Mae T. Sumaya +6 more
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2012
DNA barcoding refers to the technique of sequencing a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene, the "DNA barcode," from a taxonomically unknown specimen and performing comparisons with a reference library of barcodes of known species origin to establish a species-level identification. The library barcodes gain their
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DNA barcoding refers to the technique of sequencing a short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene, the "DNA barcode," from a taxonomically unknown specimen and performing comparisons with a reference library of barcodes of known species origin to establish a species-level identification. The library barcodes gain their
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2008
The Barcode of Life initiative represents an ambitious effort to develop an identification system for eukaryotic life based on the analysis of sequence diversity in short, standardized gene regions. Work is furthest advanced for members of the animal kingdom.
Paul D. N. Hebert +3 more
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The Barcode of Life initiative represents an ambitious effort to develop an identification system for eukaryotic life based on the analysis of sequence diversity in short, standardized gene regions. Work is furthest advanced for members of the animal kingdom.
Paul D. N. Hebert +3 more
openaire +2 more sources
DNA Barcoding Amphibians and Reptiles
2012Only a few major research programs are currently targeting COI barcoding of amphibians and reptiles (including chelonians and crocodiles), two major groups of tetrapods. Amphibian and reptile species are typically old, strongly divergent, and contain deep conspecific lineages which might lead to problems in species assignment with incomplete reference ...
Miguel Vences +3 more
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