Results 121 to 130 of about 73,396 (304)

Shiga Toxin Detection Methods : A Short Review [PDF]

, 2013
The Shiga toxins comprise a family of related protein toxins secreted by certain types of bacteria. Shigella dysenteriae, some strain of Escherichia coli and other bacterias can express toxins which caused serious complication during the infection. Shiga
González-Aguilar, G., Guerrero, Y. Castaño   +1 more
core  

De Novo Design and Directed Evolution Refinement of Mirror‐Image Protein Binders Targeting Interleukin‐4

Advanced Science, EarlyView.
This study presents the de novo design and directed evolution of a mirror‐image D‐protein inhibitor targeting human interleukin‐4 (IL‐4). The engineered molecule exhibits nanomolar binding affinity for IL‐4 and effectively inhibits IL‐4–mediated signaling.
Liqing Xu, Yuxiang Ren, Tongyue Wang, Hongyong Li, Yue Yao, Lei Liu, Ke Sun, Peilong Lu   +7 more
wiley   +1 more source

Waveguide-Enhanced Nanoplasmonic Biosensor for Ultrasensitive and Rapid DNA Detection

Micromachines
DNA is fundamental for storing and transmitting genetic information. Analyzing DNA or RNA base sequences enables the identification of genetic disorders, monitoring gene expression, and detecting pathogens.
Devesh Barshilia, Akhil Chandrakanth Komaram, Lai-Kwan Chau, Guo-En Chang   +3 more
doaj   +1 more source

An Artificial Antibody‐Based Toolbox Accelerates Validation of Hidden Microproteins Encoded by the Dark Genome

Advanced Science, EarlyView.
Microproteins are hidden treasures encoded by the “dark proteome” but remain largely underexplored due to the lack of highly efficient tools. We developed a molecularly imprinted polymers (MIPs)‐based toolbox (CLAIMID) to achieve accelerated and ultrasensitive microproteins validation at multiple biological scales (single living cells, cell populations,
Hui He, Anqi Zhang, Zhanchen Guo, Qi Li, Song Gao, Wenyan Guan, Ying Li, Jingran Chen, Sijia Sun, Jian He, Zhen Liu   +10 more
wiley   +1 more source

Construction of a nrdA::luxCDABE Fusion and Its Use in Escherichia coli as a DNA Damage Biosensor

Sensors, 2008
The promoter of nrdA gene which is related with DNA synthesis was used to construct a DNA damage sensitive biosensor. A recombinant bioluminescent E.
Man Bock Gu, Byoung Chan Kim, Joo- Myung Ahn, Ee Taek Hwang   +3 more
doaj  

A nanoscale electrochemical guanine DNA-biosensor based on a flower-like nanocomposite of Tb-doped ZnO for the sensitive determination of pemetrexed. [PDF]

RSC Adv, 2023
Abbasi M, Jahani S, Biroudian S, Boroujeni MA, Maghfoury F, Amini-Zadeh M, Malekyan L, Faramarzpoor HR, Foroughi MM.   +8 more
europepmc   +1 more source

Single‐Cell Transcriptomics Reveals FLS2‐Dependent Hypoxia Signaling and ERF13‐Mediated Transcription During flg22‐Triggered Immunity

Advanced Science, EarlyView.
This study employs sc‐RNA sequencing, genetics, and phenotyping to systematically map the cell‐type‐specific immune responses triggered by flg22. It reveals FLS2‐dependent transcriptional reprogramming in epidermal and mesophyll cells, and uncovers crosstalk between immune and hypoxia signaling pathways.
Yaping Zhou, Aizhi Qin, Mengfan Li, Qianli Zhao, Luyao Kong, Lulu Yan, Chunyang Li, Hao Liu, Yinpeng Zhang, Jiani Long, Mengyu Liao, Mengmeng Zhou, Xiaoli Fan, Baozhen Wang, Wenxuan Kang, Shui Wang, Zhixin Liu, Xuwu Sun   +17 more
wiley   +1 more source

Label free electrochemical DNA biosensor for COVID-19 diagnosis. [PDF]

Talanta, 2023
Lomae A, Preechakasedkit P, Hanpanich O, Ozer T, Henry CS, Maruyama A, Pasomsub E, Phuphuakrat A, Rengpipat S, Vilaivan T, Chailapakul O, Ruecha N, Ngamrojanavanich N.   +12 more
europepmc   +1 more source

Enzymatic DNA Reaction Networks for Orchestrating Stimuli‐Dependent Temporal Molecular Pulse

Advanced Science, EarlyView.
We present an enzymatic DNA reaction network (EDRN) that encodes nucleic‐acid targets in time, converting inputs into a universal strand and then into programmable transient fluorescence pulses. With time‐color multiplexing, EDRN enables single‐tube high‐plex nucleic acid detection and shows strong agreement with clinical sequencing across 32 specimens.
Jiayu Yang, Yali Chang, Zibin Chu, Linghao Zhang, Tengfang Zhang, Dai Erhei, Xin Su, Zhe Yin   +7 more
wiley   +1 more source

Conductive Ink-Coated Paper-Based Supersandwich DNA Biosensor for Ultrasensitive Detection of Neisseria gonorrhoeae. [PDF]

Biosensors (Basel), 2023
Gupta N, Kumar D, Das A, Sood S, Malhotra BD.   +4 more
europepmc   +1 more source