Clamp loader ATPases and the evolution of DNA replication machinery
BMC Biology, 2012 Clamp loaders are pentameric ATPases of the AAA+ family that operate to ensure processive DNA replication. They do so by loading onto DNA the ring-shaped sliding clamps that tether the polymerase to the DNA.
Kelch Brian A +3 more
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Structure of an archaeal PCNA1-PCNA2-FEN1 complex: elucidating PCNA subunit and client enzyme specificity. [PDF]
, 2006 The archaeal/eukaryotic proliferating cell nuclear antigen (PCNA) toroidal clamp interacts with a host of DNA modifying enzymes, providing a stable anchorage and enhancing their respective processivities.
Andrew S. Doré +48 more
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Biochemical characterization of DNA damage checkpoint complexes: clamp loader and clamp complexes with specificity for 5' recessed DNA. [PDF]
PLoS Biology, 2003 The cellular pathways involved in maintaining genome stability halt cell cycle progression in the presence of DNA damage or incomplete replication. Proteins required for this pathway include Rad17, Rad9, Hus1, Rad1, and Rfc-2, Rfc-3, Rfc-4, and Rfc-5 ...
Viola Ellison, Bruce Stillman
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TFE and Spt4/5 open and close the RNA polymerase clamp during the transcription cycle [PDF]
, 2016 Transcription is an intrinsically dynamic process and requires the coordinated interplay of RNA polymerases (RNAPs) with nucleic acids and transcription factors.
Gietl, Andreas +5 more
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Reconstitution of recombination-associated DNA synthesis with human proteins. [PDF]
, 2013 The repair of DNA breaks by homologous recombination is a high-fidelity process, necessary for the maintenance of genome integrity. Thus, DNA synthesis associated with recombinational repair must be largely error-free.
Grossi, Sara M +4 more
core +1 more source
M. tuberculosis sliding β-clamp does not interact directly with the NAD+-dependent DNA ligase.
PLoS ONE, 2012 The sliding β-clamp, an important component of the DNA replication and repair machinery, is drawing increasing attention as a therapeutic target. We report the crystal structure of the M. tuberculosis β-clamp (Mtbβ-clamp) to 3.0 Å resolution. The protein
Vandna Kukshal +5 more
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Structural visualization of key steps in human transcription initiation. [PDF]
, 2013 Eukaryotic transcription initiation requires the assembly of general transcription factors into a pre-initiation complex that ensures the accurate loading of RNA polymerase II (Pol II) at the transcription start site. The molecular mechanism and function
Fang, Jie +3 more
core +1 more source
Searching for Low Probability Opening Events in a DNA Sliding Clamp
Life, 2022 The β subunit of E. coli DNA polymererase III is a DNA sliding clamp associated with increasing the processivity of DNA synthesis. In its free form, it is a circular homodimer structure that can accomodate double-stranded DNA in a nonspecific manner.
Reza Esmaeeli +2 more
doaj +1 more source
The Rad4TopBP1 ATR-Activation domain functions in G1/S phase in a chromatin-dependent manner [PDF]
, 2012 DNA damage checkpoint activation can be subdivided in two steps: initial activation and signal amplification. The events distinguishing these two phases and their genetic determinants remain obscure.
Carr, Antony +8 more
core +7 more sources
mBio, 2023 African swine fever virus (ASFV) is a complex nucleocytoplasmic, large DNA virus that infects both domestic pigs and wild boar, but little is known about the process of genomic replication. The E301R protein (pE301R) from ASFV was previously predicted as
Su Li +16 more
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