Results 21 to 30 of about 112,962 (287)

MutS regulates access of the error-prone DNA polymerase Pol IV to replication sites: a novel mechanism for maintaining replication fidelity [PDF]

, 2016
Translesion DNA polymerases (Pol) function in the bypass of template lesions to relieve stalled replication forks but also display potentially deleterious mutagenic phenotypes that contribute to antibiotic resistance in bacteria and lead to human disease.
Argaraña, Carlos Enrique, Fernández, Marisa Mariel, Malchiodi, Emilio Luis, Margara, Lucía, Monti, Mariela Roxana   +4 more
core   +1 more source

cryo-EM structures of the E. coli replicative DNA polymerase reveal its dynamic interactions with the DNA sliding clamp, exonuclease and τ

eLife, 2015
The replicative DNA polymerase PolIIIα from Escherichia coli is a uniquely fast and processive enzyme. For its activity it relies on the DNA sliding clamp β, the proofreading exonuclease ε and the C-terminal domain of the clamp loader subunit τ.
Rafael Fernandez-Leiro, Julian Conrad, Sjors HW Scheres, Meindert H Lamers   +3 more
doaj   +1 more source

Functional compartmentalization of Rad9 and Hus1 reveals diverse assembly of the 9-1-1 complex components during the DNA damage response in Leishmania [PDF]

, 2016
The Rad9-Rad1-Hus1 (9-1-1) complex is a key component in the coordination of DNA damage sensing, cell cycle progression and DNA repair pathways in eukaryotic cells. This PCNA-related trimer is loaded onto RPA-coated single stranded DNA and interacts with
Damasceno, Jeziel D., McCulloch, Richard, Obonaga, Ricardo, Santos, Elaine V., Scott, Alan, Tosi, Luiz R.O.   +5 more
core   +1 more source

Three DNA polymerases, recruited by different mechanisms, carry out NER repair synthesis in human cells [PDF]

, 2010
Nucleotide excision repair (NER) is the most versatile DNA repair system that deals with the major UV photoproducts in DNA, as well as many other DNA adducts.
Cloney, Ross, Fousteri, Maria I, Japers, Nicolaas G, Lehmann, Alan R, Limsirichaikul, Siripan, Miki, Yoshio, Mullenders., Leon H F, Nakazawa, Yuka, Niimi, Atsuko, Ogi, Tomoo, Overmeer, René M, Takenaka, Katsuya, Volker, Marcel, Yamashita, Shunichi   +13 more
core   +3 more sources

Allosteric communication in DNA polymerase clamp loaders relies on a critical hydrogen-bonded junction

eLife, 2021
Clamp loaders are AAA+ ATPases that load sliding clamps onto DNA. We mapped the mutational sensitivity of the T4 bacteriophage sliding clamp and clamp loader by deep mutagenesis, and found that residues not involved in catalysis or binding display ...
Subu Subramanian, Kent Gorday, Kendra Marcus, Matthew R Orellana, Peter Ren, Xiao Ran Luo, Michael E O'Donnell, John Kuriyan   +7 more
doaj   +1 more source

Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA [PDF]

, 2013
In eukaryotes, the Cdt1-bound replicative helicase core MCM2-7 is loaded onto DNA by the ORC-Cdc6 ATPase to form a prereplicative complex (pre-RC) with an MCM2-7 double hexamer encircling DNA.
A Costa, A Fernández-Cid, Alberto Riera, Alejandra Fernández-Cid, AS Brewster, AY Lyubimov, B Stillman, BA Kelch, BI Khayrutdinov, Bruce Stillman, C Evrin, C Evrin, C Liu, C Santocanale, C Speck, C Speck, Cecile Evrin, Christian Speck, D Remus, D Remus, D Suck, E Arias-Palomo, EF Pettersen, EL Dueber, G Tang, GC Lander, GD Bowman, GD Pintilie, H Li, H Li, Hironori Kawakami, Huilin Li, I Ilves, IN Chesnokov, J Frigola, J Liu, J Sun, JC Randell, Jingchuan Sun, JL Bowers, L Zou, M Gaudier, M O'Donnell, MG Clarey, MG Clarey, ML Baker, ML Bochman, ML Bochman, ML Bochman, PW Lau, R Henderson, RC Heller, RD Klemm, RJ Fletcher, S Chen, S Donovan, S Onesti, S Waga, SE Moyer, SL Forsburg, SP Bell, SP Bell, Stefan A Samel, T Miyata, T Pape, Y Ishimi, Y Kawasaki, YH Kang, Z Chen   +68 more
core   +1 more source

The crystal structure of Haloferax volcanii proliferating cell nuclear antigen reveals unique surface charge characteristics due to halophilic adaptation [PDF]

, 2009
Background: The high intracellular salt concentration re quired to maintain a halophilic lifestyle poses challenges to haloarchaeal proteins that must stay soluble, stable and functional in this extreme environment.
Bunting, KA, Christofi, P, Morroll, S, Winter, JA   +3 more
core   +2 more sources

A Replicase Clamp-Binding Dynamin-like Protein Promotes Colocalization of Nascent DNA Strands and Equipartitioning of Chromosomes in E. coli

Cell Reports, 2013
In Escherichia coli, bidirectional chromosomal replication is accompanied by the colocalization of sister replication forks. However, the biological significance of this mechanism and the key factors involved are still largely unknown.
Shogo Ozaki, Yusaku Matsuda, Kenji Keyamura, Hironori Kawakami, Yasunori Noguchi, Kazutoshi Kasho, Komomo Nagata, Tamami Masuda, Yukari Sakiyama, Tsutomu Katayama   +9 more
doaj   +1 more source

Rings in the Extreme: PCNA Interactions and Adaptations in the Archaea [PDF]

, 2012
Biochemical and structural analysis of archaeal proteins has enabled us to gain great insight into many eukaryotic processes, simultaneously offering fascinating glimpses into the adaptation and evolution of proteins at the extremes of life. The archaeal
Bunting, KA, Winter, JA
core   +2 more sources

Role of the Checkpoint Clamp in DNA Damage Response

Biomolecules, 2013
DNA damage occurs during DNA replication, spontaneous chemical reactions, and assaults by external or metabolism-derived agents. Therefore, all living cells must constantly contend with DNA damage.
Mihoko Kai
doaj   +1 more source