PLoS ONE, 2018 The nick translation property of DNA polymerase I (Pol I) ensures the maturation of Okazaki fragments by removing primer RNAs and facilitating ligation. However, prolonged nick translation traversing downstream DNA is an energy wasting futile process, as
Amit Bhardwaj +3 more
doaj +1 more source
A dnaN plasmid shuffle strain for rapid in vivo analysis of mutant Escherichia coli β clamps provides insight into the role of clamp in umuDC-mediated cold sensitivity. [PDF]
PLoS ONE, 2014 The E. coli umuDC gene products participate in two temporally distinct roles: UmuD2C acts in a DNA damage checkpoint control, while UmuD'2C, also known as DNA polymerase V (Pol V), catalyzes replication past DNA lesions via a process termed translesion ...
Vignesh M P Babu, Mark D Sutton
doaj +1 more source
Herpes simplex virus type 1 single strand DNA binding protein and helicase/primase complex disable cellular ATR signaling. [PDF]
PLoS Pathogens, 2013 Herpes Simplex Virus type 1 (HSV-1) has evolved to disable the cellular DNA damage response kinase, ATR. We have previously shown that HSV-1-infected cells are unable to phosphorylate the ATR substrate Chk1, even under conditions in which replication ...
Kareem N Mohni +4 more
doaj +1 more source
A CTP-dependent gating mechanism enables ParB spreading on DNA
eLife, 2021 Proper chromosome segregation is essential in all living organisms. The ParA-ParB-parS system is widely employed for chromosome segregation in bacteria.
Adam SB Jalal +6 more
doaj +1 more source
Nucleotide-dependent DNA gripping and an end-clamp mechanism regulate the bacteriophage T4 viral packaging motor. [PDF]
, 2018 ATP-powered viral packaging motors are among the most powerful biomotors known. Motor subunits arranged in a ring repeatedly grip and translocate the DNA to package viral genomes into capsids.
Alam, Istiaq +4 more
core +3 more sources
Stepwise assembly of the human replicative polymerase holoenzyme
eLife, 2013 In most organisms, clamp loaders catalyze both the loading of sliding clamps onto DNA and their removal. How these opposing activities are regulated during assembly of the DNA polymerase holoenzyme remains unknown.
Mark Hedglin +3 more
doaj +1 more source
The DNA damage checkpoint pathway promotes extensive resection and nucleotide synthesis to facilitate homologous recombination repair and genome stability in fission yeast. [PDF]
, 2014 DNA double-strand breaks (DSBs) can cause chromosomal rearrangements and extensive loss of heterozygosity (LOH), hallmarks of cancer cells. Yet, how such events are normally suppressed is unclear.
Blaikley, EJ +13 more
core +1 more source
Structural characterisation of the complete cycle of sliding clamp loading in Escherichia coli
Nature CommunicationsRing-shaped DNA sliding clamps are essential for DNA replication and genome maintenance. Clamps need to be opened and chaperoned onto DNA by clamp loader complexes (CLCs).
Zhi-Qiang Xu +10 more
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DNA Sliding Clamps as Therapeutic Targets
Frontiers in Molecular Biosciences, 2018 Chromosomal DNA replication is achieved by an assembly of multi-protein complexes at the replication fork. DNA sliding clamps play an important role in this assembly and are essential for cell viability. Inhibitors of bacterial (β-clamp) and eukaryal DNA
Amanda S. Altieri +2 more
doaj +1 more source
A Single-Strand Annealing Protein Clamps DNA to Detect and Secure Homology. [PDF]
PLoS Biology, 2015 Repair of DNA breaks by single-strand annealing (SSA) is a major mechanism for the maintenance of genomic integrity. SSA is promoted by proteins (single-strand-annealing proteins [SSAPs]), such as eukaryotic RAD52 and λ phage Redβ.
Marcel Ander +4 more
doaj +1 more source