Results 51 to 60 of about 112,962 (287)

PNA-Dependent Gene Chemistry: Stable Coupling of Peptides and Oligonucleotides to Plasmid DNA

BioTechniques, 2000
Two approaches are described for stably conjugating peptides, proteins and oligonucleotides onto plasmid DNA. Both methods use a peptide nucleic acid (PNA) clamp, which binds irreversibly and specifically to a binding site cloned into the plasmid.
O. Zelphati, X. Liang, C. Nguyen, S. Barlow, S. Sheng, Z. Shao, P.L. Felgner   +6 more
doaj   +1 more source

DNA polymerase III protein, HolC, helps resolve replication/transcription conflicts

Microbial Cell, 2021
In Escherichia coli, DNA replication is catalyzed by an assembly of proteins, the DNA polymerase III holoenzyme. This complex includes the polymerase and proofreading subunits, the processivity clamp and clamp loader complex.
Susan T. Lovett
doaj   +1 more source

Harnessing Non‐Covalent Protein–Protein Interaction Domains for Production of Biocatalytic Materials Systems

Advanced Functional Materials, EarlyView.
Non‐covalent protein–protein interactions mediated by SH3, PDZ, or GBD domains enable the self‐assembly of stable and biocatalytically active hydrogel materials. These soft materials can be processed into monodisperse foams that, once dried, exhibit enhanced mechanical stability and activity and are easily integrated into microstructured flow ...
Julian S. Hertel, Maria Alessandra Martini, Marius Stoeckle, Annika J. Weber, Kersten S. Rabe, Christof M. Niemeyer   +5 more
wiley   +1 more source

The Role of Replication Clamp-Loader Protein HolC of Escherichia coli in Overcoming Replication/Transcription Conflicts

mBio, 2021
Transcription elongation complexes present an impediment to DNA replication. We provide evidence that one component of the replication clamp loader complex, HolC, of Escherichia ...
Deani L. Cooper, Taku Harada, Samia Tamazi, Alexander E. Ferrazzoli, Susan T. Lovett   +4 more
doaj   +1 more source

A Smart Bio‐Battery Facilitates Diabetic Bone Defect Repair Via Inducing Macrophage Reprogramming and Synergistically Modulating Bone Remodeling Coupling

Advanced Functional Materials, EarlyView.
This research presents a novel implantable bio‐battery, GF‐OsG, tailored for diabetic bone repair. GF‐OsG generates microcurrents in high‐glucose conditions to enhance vascularization, shift macrophages to the M2 phenotype, and regulate immune responses.
Nanning Lv, Haifu Sun, Wenxiang Tang, Yonggang Li, Zhonglai Qian, Lihui Hong, Chong Chen, Hongye Li, Jiaxiang Bai, Yusen Qiao, Mingming Liu   +10 more
wiley   +1 more source

Lysine residue 185 of Rad1 is a topological but not a functional counterpart of lysine residue 164 of PCNA. [PDF]

PLoS ONE, 2011
Monoubiquitylation of the homotrimeric DNA sliding clamp PCNA at lysine residue 164 (PCNA(K164)) is a highly conserved, DNA damage-inducible process that is mediated by the E2/E3 complex Rad6/Rad18.
Niek Wit, Peter H L Krijger, Paul C M van den Berk, Heinz Jacobs   +3 more
doaj   +1 more source

Multiplexed DNA-Modified Electrodes [PDF]

, 2010
We report the use of silicon chips with 16 DNA-modified electrodes (DME chips) utilizing DNA-mediated charge transport for multiplexed detection of DNA and DNA-binding protein targets.
Barton, Jacqueline K., Gorodetsky, Alon A., Muren, Natalie B., Slinker, Jason D.   +3 more
core   +2 more sources

Laser‐Based Sculpturing of Embedded Ultrathin Metal‐Oxide Nanopores for Enhanced Biomolecular Sensing

Advanced Functional Materials, EarlyView.
Controlled laser‐drilling of embedded HfO2 membranes creates three layer nanopores with Gaussian‐shaped cavities sculptured in the supporting layers. These embedded solid‐state nanopores slow DNA translocation by 12‐fold compared to SiNx pores, enabling high‐resolution, label‐free detection of short DNAs, RNAs, and proteins.
Jostine Joby, Yulia Marom, Guocong Lin, Aleksandra Baron‐Wiechec, Amit Meller   +4 more
wiley   +1 more source

Emergence of highly profibrotic and proinflammatory Lrat+Fbln2+ HSC subpopulation in alcoholic hepatitis

Hepatology, EarlyView., 2022
Lrat+ quiescent hepatic stellate cells (qHSC) give rise to Lrat+Fbln2+ activated HSC (aHSC) in alcohol‐associated hepatitis and this subpopulation is highly profibrotic, inflammatory, and immunoregulatory based on their single cell transcriptomic profile. Abstract Background and Aims Relative roles of HSCs and portal fibroblasts in alcoholic hepatitis (
Steven Balog, Reika Fujiwara, Stephanie Q. Pan, Khairat B. El‐Baradie, Hye Yeon Choi, Sonal Sinha, Qihong Yang, Kinji Asahina, Yibu Chen, Meng Li, Matthew Salomon, Stanley W.‐K. Ng, Hidekazu Tsukamoto   +12 more
wiley   +1 more source

The Ctf18 RFC-like complex positions yeast telomeres but does not specify their replication time [PDF]

, 2006
Peer ...
Andrulis ED, Anne D Donaldson, Askree SH, Bermudez VP, Boulton SJ, Bowman GD, Bylund GO, Bystricky K, Cosgrove AJ, Dimitrova DS, Donaldson AD, E Douglas Robertson, Ferguson BM, Gartenberg MR, Gilbert DM, Gilbert N, Gotta M, Gottschling DE, Gravel S, Hanna JS, Hayashi A, Hediger F, Heun P, Heun P, Kenna MA, Kim J, Laroche T, Martin SG, Mayer ML, Merkle CJ, Naiki T, Ohta S, Palladino F, Petronczki M, Porter SE, Raghuraman MK, Raghuraman MK, Roy R, Shin-ichiro Hiraga, Skibbens RV, Smolikov S, Stellwagen AE, Stevenson JB, Strahl-Bolsinger S, Straight AF, Suter B, Taddei A, Taddei A, Tanabe H, Tanaka T, Tham WH, Woodfine K   +51 more
core   +2 more sources