Results 81 to 90 of about 112,962 (287)

Template-directed biopolymerization: tape-copying Turing machines

, 2012
DNA, RNA and proteins are among the most important macromolecules in a living cell. These molecules are polymerized by molecular machines. These natural nano-machines polymerize such macromolecules, adding one monomer at a time, using another linear ...
Chowdhury, Debashish, Sharma, Ajeet K.
core   +1 more source

An Injection‐Molded Modified Silicone Rubber for Cancer‐on‐Chip Applications

Advanced Materials Technologies, Volume 10, Issue 6, March 18, 2025.
This study describes the characterization and application of an injection‐molded functionalized PDMS fluidic insert for the commercially available Micronit system. Modified PDMS can accommodate cell culture and is able to covalently bind proteins for long‐lasting coatings under flow‐conditions.
Ben J. Haspels, Frederikus Bakker, Lucas J. A. M. Beckers, Roland Kanaar, Koen C. Kriege, Susanne M. Valster, Roland C. M. Vulders, Maayke M. P. Kuijten   +7 more
wiley   +1 more source

Stretching of proteins in a uniform flow

, 2006
Stretching of a protein by a fluid flow is compared to that in a force-clamp apparatus. The comparison is made within a simple topology-based dynamical model of a protein in which the effects of the flow are implemented using Langevin dynamics.
Chan E. Y., Marek Cieplak, P. Szymczak
core   +1 more source

Multiple prebiotic metals mediate translation. [PDF]

, 2018
Today, Mg2+ is an essential cofactor with diverse structural and functional roles in life's oldest macromolecular machine, the translation system. We tested whether ancient Earth conditions (low O2, high Fe2+, and high Mn2+) can revert the ribosome to a ...
Bowman, Jessica C, Bray, Marcus S, Glass, Jennifer B, Haynes, Jay William, Hud, Nicholas V, Lenz, Timothy K, Petrov, Anton S, Reddi, Amit R, Williams, Loren Dean   +8 more
core   +1 more source

Femtosecond‐Laser‐Fabricated 3D CYTOP Microfluidic Chips Enabling Super‐Resolution Live Imaging of Cell Behavior in Confined Microspaces

Advanced Materials Technologies, EarlyView.
Using two‐photon polymerization of SU‐8 together with mold processing allowed the creation of defect‐free, flexible 3D microfluidic structures with sub‐micrometer precision in CYTOP. The fabricated CYTOP microfluidic chips enabled super‐resolution imaging of cancer cells in the microchannels, clearly visualizing previously undetectable fine structural ...
Koji Sugioka, Mirai Hanzawa, Kotaro Obata, Sota Matsumoto, Masatoshi Takagi, Asako Sakaue‐Sawano, Asako Tosaki, Satoshi Shimozono, Felix Sima, Hiroyuki Kawano, Takuro Tojima, Daisuke Miyashiro, Kazunari Ozasa, Yasutaka Hanada, Godai Miyaji, Akihiko Nakano, Atsushi Miyawaki   +16 more
wiley   +1 more source

Single-molecule studies contrast ordered DNA replication with stochastic translesion synthesis

eLife, 2017
High fidelity replicative DNA polymerases are unable to synthesize past DNA adducts that result from diverse chemicals, reactive oxygen species or UV light.
Gengjing Zhao, Emma S Gleave, Meindert Hugo Lamers   +2 more
doaj   +1 more source

Circular RNA PTPN4 Contributes to Blood‐Brain Barrier Disruption during Early Epileptogenesis

Advanced Science, EarlyView.
Epileptic condition induces CircPTPN4 upregulation, which promotes ECE‐1 expression through competitive sequestration of miR‐145a‐5p. The elevated ECE‐1 catalyzes the ET‐1 production, leading to p38/MAPK pathway activation and subsequent downregulation of tight junction protein expression. This cascade results in increased BBB permeability and enhanced
Jiurong Yang, Yang Hu, Feiyu Wang, Xintao Peng, Honggang Qi, Yuanyuan Yao, Canyu Zhang, Lijie Zhou, Xuemei Liang, Kang Xu, Cong Zhang, Aifeng Zhang, Chen Chen, Yu Zeng, Chenchen Zhang, Guangming Gan, Xinjian Zhu   +16 more
wiley   +1 more source

Assembly and functional analysis of an S/MAR based episome with the cystic fibrosis transmembrane conductance regulator gene [PDF]

, 2018
Improving the efficacy of gene therapy vectors is still an important goal toward the development of safe and efficient gene therapy treatments. S/MAR (scaffold/matrix attached region)-based vectors are maintained extra-chromosomally in numerous cell ...
Ascenzioni, Fiorentina, Castellani, Stefano, Cavinato, Luca, Cimino, Giuseppe, Conese, Massimo, De Rocco, Davide, Guarnieri, Simone, Mariggiò, Maria A., Morini, Elena, Pompili, Barbara, Porto, Paola Del   +10 more
core   +3 more sources

Annexin A13 Protects Against Acute Kidney Injury by Inactivating TGF‐β/Smad3 Signaling

Advanced Science, EarlyView.
ANXA13 is negatively regulated by Smad3 and exerts its protective role in AKI by inactivating TGF‐β/Smad3 signaling and Smad3‐p21 cell cycle arrest pathway through binding to TβRI, inhibiting the interaction between TβRI and TβRII, thereby suppressing TβRI phosporylation.
Jiaxiao Li, Chen Wu, Yuqi Zhu, Zicheng Liu, Wenjuan Yu, Andrew Lukwaro, Yu Zhong, Guoqiang Xie, Lili Zhou, Xiaoru Huang, Hui‐yao Lan, Junzhe Chen, Ying Tang   +12 more
wiley   +1 more source

Structural and Dynamic Features of the Recognition of 8-oxoguanosine Paired with an 8-oxoG-clamp by Human 8-oxoguanine-DNA Glycosylase

Current Issues in Molecular Biology
8-oxoguanine (oxoG) is formed in DNA by the action of reactive oxygen species. As a highly mutagenic and the most common oxidative DNA lesion, it is an important marker of oxidative stress. Human 8-oxoguanine-DNA glycosylase (OGG1) is responsible for its
Maria V. Lukina, Polina V. Zhdanova, Vladimir V. Koval   +2 more
doaj   +1 more source