Results 81 to 90 of about 8,402,765 (253)

MRE11 facilitates the removal of human topoisomerase II complexes from genomic DNA [PDF]

, 2012
Topoisomerase II creates a double-strand break intermediate with topoisomerase covalently coupled to the DNA via a 5'-phosphotyrosyl bond. These intermediate complexes can become cytotoxic protein-DNA adducts and DSB repair at these lesions requires ...
Caroline A. Austin, Graham H. Jackson, Nicholas J. Morris, Zbyslaw Sondka, Tainer, JA, Padget, Kay, Simon J. Cockell, Curtis, H, Cockell, SJ, Curtis, Hannah, Sondka, Zbyslaw, Austin, CA, John A. Tainer, Cowell, IG, Austin, Caroline, Padget, K, Morris, Nicholas, Moiani, Davide, Cowell, Ian, Ian G. Cowell, Sondka, Z, Lee, KC, Tainer, John, Jackson, Graham, Kay Padget, Moiani, D, Lee, Ka Cheong, Jackson, GH, Ka Cheong Lee, Davide Moiani, Hannah Curtis, Morris, NJ, Cockell, Simon   +32 more
core   +1 more source

DNA endoreplication level in endosperm and in cotyledons during seed development in three dicotyledonous species

Acta Societatis Botanicorum Poloniae, 2014
The DNA content of nuclear and cellular endosperm and in cotyledons in the course of seed development in Helianthus annuus, Pisum sativum and Vicia faba was cytophotometrically measured after Faulgen's reaction.
Kazimierz Marciniak
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Ochratoxin A: In Utero Exposure in Mice Induces Adducts in Testicular DNA [PDF]

, 2010
Ochratoxin A (OTA) is a nephrotoxin and carcinogen that is associated with Balkan endemic nephropathy and urinary tract tumors. OTA crosses the placenta and causes adducts in the liver and kidney DNA of newborns.
Jennings-Gee, Jamie E., Mark Steven Miller, Tozlovanu, Mariana, Jamie E. Jennings-Gee, Mariana Tozlovanu, Miller, Mark Steven, Annie Pfohl-Leszkowicz, Gary G. Schwartz, Schwartz, Gary G., Manderville, Richard A., Pfohl-Leszkowicz, Annie, Richard Manderville   +11 more
core   +1 more source

Measuring DNA content in live cells by fluorescence microscopy

Cell Division, 2018
Background Live-cell fluorescence microscopy (LCFM) is a powerful tool used to investigate cellular dynamics in real time. However, the capacity to simultaneously measure DNA content in cells being tracked over time remains challenged by dye-associated ...
Cecil J. Gomes, Michael W. Harman, Sara M. Centuori, Charles W. Wolgemuth, Jesse D. Martinez   +4 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Proof of principle for shotgun DNA mapping by unzipping [PDF]

, 2009
Ability to map polymerases and nucleosomes on chromatin is important for understanding the impact of chromatin remodeling on key cellular processes. Current methods (such as ChIP and ChIP-chip) have produced a wealth of information that demonstrates ...
Steven J. Koch, Herskowitz, Lawrence J., Steven Koch, Linh Le, Salvagno, Anthony L., Anthony L. Salvagno, R. Andy Maloney, Le, Linh, Linh N. Le, Lawrence J. Herskowitz, Anthony Salvagno, Koch, Steven J., Lawrence Herskowitz   +12 more
core   +1 more source

Assessment of Mitochondrial DNA Content and Mass in Macrophages

Bio-Protocol, 2016
Mitochondria are essential regulators in not only ATP generation and metabolic reprogramming but also the generation of reactive oxygen species (ROS) in response to pathogenic stimuli. During exposure to environmental stresses including oxidative stress,
Jae-Min Yuk, Tae Kim, Eun-Kyeong Jo
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source