Results 91 to 100 of about 888,680 (335)

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases

Nature Communications
Prime editing allows precise installation of any single base substitution and small insertions and deletions without requiring homologous recombination or double-strand DNA breaks in eukaryotic cells.
Hongyuan Zhang, Jiacheng Ma, Zhaowei Wu, Xiaoyang Chen, Yangyang Qian, Weizhong Chen, Zhipeng Wang, Ya Zhang, Huanhu Zhu, Xingxu Huang, Quanjiang Ji   +10 more
doaj   +1 more source

High-Resolution Analysis of the Efficiency, Heritability, and Editing Outcomes of CRISPR/Cas9-Induced Modifications of NCED4 in Lettuce (Lactuca sativa). [PDF]

, 2018
CRISPR/Cas9 is a transformative tool for making targeted genetic alterations. In plants, high mutation efficiencies have been reported in primary transformants. However, many of the mutations analyzed were somatic and therefore not heritable.
Bertier, Lien D, Bradford, Kent J, Britt, Anne B, Huo, Heqiang, Michelmore, Richard W, Ron, Mily   +5 more
core   +2 more sources

Biallelic Inactivation of NSD1 Associated With Carcinogenesis in Sotos Syndrome

Pediatric Blood &Cancer, EarlyView.
Nicholas A. Borja, Anuja Sule, Katie Ann McMullen, Aditi Dhir, Matthew D. Hall, Heather J. McCrea, Sakir Humayun Gultekin, Mustafa Tekin, Bradley Gampel   +8 more
wiley   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Characterizing and controlling CRISPR repair outcomes in nondividing human cells

Nature Communications
Genome editing is poised to revolutionize treatment of genetic diseases, but poor understanding and control of DNA repair outcomes hinders its therapeutic potential.
Gokul N. Ramadoss, Samali J. Namaganda, Manasi M. Kumar, Jennifer R. Hamilton, Rohit Sharma, Karena G. Chow, Luke A. Workley, Bria L. Macklin, Mengyuan Sun, Alvin S. Ha, Jia-Cheng Liu, Christof Fellmann, Hannah L. Watry, Philip H. Dierks, Rudra S. Bose, Julianne Jin, Barbara S. Perez, Cindy R. Sandoval Espinoza, Madeline P. Matia, Serena H. Lu, Luke M. Judge, Brian R. Shy, Andre Nussenzweig, Britt Adamson, Niren Murthy, Jennifer A. Doudna, Martin Kampmann, Bruce R. Conklin   +27 more
doaj   +1 more source

A model for codon position bias in RNA editing [PDF]

, 2005
RNA editing can be crucial for the expression of genetic information via inserting, deleting, or substituting a few nucleotides at specific positions in an RNA sequence. Within coding regions in an RNA sequence, editing usually occurs with a certain bias
Bundschuh, Ralf, Liu, Tsunglin
core   +2 more sources

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

DEMINING: A deep learning model embedded framework to distinguish RNA editing from DNA mutations in RNA sequencing data

Genome Biology
Precise calling of promiscuous adenosine-to-inosine RNA editing sites from transcriptomic datasets is hindered by DNA mutations and sequencing/mapping errors.
Zhi-Can Fu, Bao-Qing Gao, Fang Nan, Xu-Kai Ma, Li Yang   +4 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source