Results 191 to 200 of about 888,680 (335)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

DNA-editing enzymes as potential treatments for heteroplasmic mtDNA diseases. [PDF]

J Intern Med, 2020
Zekonyte U, Bacman SR, Moraes CT.
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

DNA, SESSO DELLE MOSCHE E GENE EDITING: PROTEGGERE LA FRUTTA SENZA PESTICIDI

, 2022
Giuseppe Saccone
openalex   +2 more sources

Domain associated with zinc fingers‐containing NF90‐NF45 complex inhibits m6A modification of primary microRNA by suppressing METTL3/14 activity

FEBS Open Bio, EarlyView.
NF90–NF45 functions as a negative regulator of methyltransferase‐like 3/14 (METTL3/14)‐mediated N6‐methyladenosine (m6A) modification on primary microRNAs (pri‐miRNAs). NF90–NF45 binds to anti‐oncogenic pri‐miRNAs and inhibits their m6A modification, thereby suppressing the biogenesis of anti‐oncogenic miRNAs.
Takuma Higuchi, Shunsuke Morioka, Keiko Morisawa, Kazutsugu Matsukawa, Shingo Ashida, Takeshi Suzuki, Shuji Sakamoto   +6 more
wiley   +1 more source

Programming PAM antennae for efficient CRISPR-Cas9 DNA editing. [PDF]

Sci Adv, 2020
Wang F, Hao Y, Li Q, Li J, Zhang H, Zhang X, Wang L, Bustamante C, Fan C.   +8 more
europepmc   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

MagnEdit-interacting factors that recruit DNA-editing enzymes to single base targets. [PDF]

Life Sci Alliance, 2020
McCann JL, Salamango DJ, Law EK, Brown WL, Harris RS.   +4 more
europepmc   +1 more source

Faculty Opinions recommendation of Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans.

, 2019
Ronald Ellis
openalex   +2 more sources

Structural basis for Cas9-mediated DNA interrogation and editing with 5′ truncated sgRNAs [PDF]

, 2023
Kaitlyn A. Kieman, Jieun Kwon, Melissa Munoz, Bradley Merill, Miljan Siminovic   +4 more
openalex   +1 more source