Results 141 to 150 of about 4,713,939 (389)

Preparation of Mealybugs (Hemiptera: Pseudococcidae) for Genetic Characterization and Morphological Examination. [PDF]

open access: yes, 2015
Mealybugs (Hemiptera: Pseudococcidae) are economically significant agricultural pests on many different crops. Because of their small size and lack of easily visible characters for identification, determination of their taxonomic status is difficult and ...
Bahder, BW   +3 more
core   +1 more source

Sterivex DNA extraction v1

open access: yes, 2020
A mostly automated protocol for extraction of genomic DNA from seawater filtered onto a Sterivex filter (Cat. No. SVGP0150). Reagents come from the Macherey-Nagel NucleoMag Plant Kit for DNA purfication (Cat. No. 744400). Automated liquid handling is performed on an eppendorf EpMotion 5075t with multi-channel pipettes.
Rob Lampe, Andrew E Allen
openaire   +2 more sources

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

open access: yesMolecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka   +10 more
wiley   +1 more source

Simplified DNA Extraction Methods for Sexing Chick Embryos

open access: yesThe Journal of Poultry Science, 2004
We investigated the efficiency of DNA extraction and the accuracy of polymerase chain reaction- (PCR-) based sexing of DNA that was obtained from adult chickens and early chicken embryos. DNA samples were prepared using water-, ammonium-, and proteinase-
Takeo Minematsu   +4 more
doaj   +1 more source

Characteristics of Alveolar Bone Marrow Cells from Patients Undergoing Dental Extractions or Dental Implant Therapy [PDF]

open access: yes, 2020
Alveolar bone marrow stromal cells (aBMSCs) play important roles in craniofacial wound healing. To establish an easy, efficient and reliable method to harvest aBMSCs, we compared three different methods: extraction socket aspiration, osteotomy aspiration
Lee, Meng H
core   +2 more sources

Ekstraksi Dna Dan Amplifikasi Its Rdna Isolat Fungi Endofit Lbkurcc67 Umbi Tanaman Dahlia (Dahlia Variabilis) [PDF]

open access: yes, 2015
> Endophyte fungi lives in the plant tissues without causing harm to their host and has known that can produce secondary metabolite and extracellular enzyme.
Charles H. Lang (318203)   +5 more
core   +3 more sources

Comparing self‐reported race and genetic ancestry for identifying potential differentially methylated sites in endometrial cancer: insights from African ancestry proportions using machine learning models

open access: yesMolecular Oncology, EarlyView.
Integrating ancestry, differential methylation analysis, and machine learning, we identified robust epigenetic signature genes (ESGs) and Core‐ESGs in Black and White women with endometrial cancer. Core‐ESGs (namely APOBEC1 and PLEKHG5) methylation levels were significantly associated with survival, with tumors from high African ancestry (THA) showing ...
Huma Asif, J. Julie Kim
wiley   +1 more source

The Case for National DNA Identification Cards [PDF]

open access: yes, 2003
Foes of the United States have demonstrated their ability to strike at the heart of this country. Fear of renewed attacks and a desire for greater national security have now prompted many to call for improvements in the national personal identification ...
Quarmby, Ben
core   +1 more source

Insect collections and DNA analyses: how to manage collections? [PDF]

open access: yes, 2008
Numerous papers have been recently published regarding DNA studies of museum insect specimens. Despite the great enthusiasm for such analyses, there continues to be a potential conflict between specimen maintenance and their use for experimental ...
MANDRIOLI, Mauro
core   +1 more source

Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples

open access: yesBMC Research Notes, 2018
Efficient and easy-to-use DNA extraction and purification methods are critical in implementing PCR-based diagnosis of pathogens. In order to optimize the routine clinical laboratory diagnosis of eukaryotic enteric pathogens, we compare, via quantitative ...
E. Menu   +5 more
semanticscholar   +1 more source

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