Results 141 to 150 of about 4,748,303 (367)
How to Process Sputum Samples and Extract Bacterial DNA for Microbiota Analysis [PDF]
, 2018 Different steps and conditions for DNA extraction for microbiota analysis in sputum have been reported in the literature. We aimed at testing both dithiothreitol (DTT) and enzymatic treatments of sputum samples and identifying the most suitable DNA ...
Andrea Gramegna +17 more
core +3 more sources
BMC Research Notes, 2018 Efficient and easy-to-use DNA extraction and purification methods are critical in implementing PCR-based diagnosis of pathogens. In order to optimize the routine clinical laboratory diagnosis of eukaryotic enteric pathogens, we compare, via quantitative ...
E. Menu +5 more
semanticscholar +1 more source
Molecular Oncology, EarlyView.We quantified and cultured circulating tumor cells (CTCs) of 62 patients with various cancer types and generated CTC‐derived tumoroid models from two salivary gland cancer patients. Cellular liquid biopsy‐derived information enabled molecular genetic assessment of systemic disease heterogeneity and functional testing for therapy selection in both ...
Nataša Stojanović Gužvić +31 more
wiley +1 more source
Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary cells in the presence of adenovirus [PDF]
, 1994 Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin ...
Baker, A. +4 more
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Automatic detection of spermatozoa for laser capture microdissection [PDF]
, 2008 In sexual assault crimes, differential extraction of spermatozoa from vaginal swab smears is often ineffective, especially when only a few spermatozoa are present in an overwhelming amount of epithelial cells.
Deforce, Dieter +3 more
core +2 more sources
Frugal method for extracting DNA from saliva suitable for low-budget labs and usable in PCR assays
Journal of BioScience and BiotechnologyBlood is the preferred source of DNA as it yields large quantities of high-quality DNA. However, there are challenges associated with this process, including difficulty in finding small and thin veins, rigid veins due to aging, and individuals with ...
Kamar Shayah +4 more
doaj +1 more source
Robust CTAB-activated charcoal protocol for plant DNA extraction
Acta Agriculturae Slovenica, 2006 DNA extracted from plants rich in polyphenols and/or polysaccharides is often problematic when subjected to polymerase chain reaction, especially when mature tissues are used for DNA extraction.
Mitja KRIŽMAN +4 more
doaj +1 more source
Optimization of DNA extraction from dental remains
Electrophoresis, 2019 Efficient DNA extraction procedures is a critical step involved in the process of successful DNA analysis of such samples. Various protocols have been devised for the genomic DNA extraction from human tissues and forensic stains, such as dental tissue ...
C. Cafiero +5 more
semanticscholar +1 more source
Molecular Oncology, EarlyView.The authors analyzed the spatial distributions of gene and metabolite profiles in cervical cancer through spatial transcriptomic and spatially resolved metabolomic techniques. Pivotal genes and metabolites within these cases were then identified and validated.
Lixiu Xu +3 more
wiley +1 more source
Species Authentication of Dog, Cat, and Tiger Using Cytochrome Β Gene [PDF]
, 2013 Adulteration of animal food products for economic reason has happened during the last decades. Species identification method development was needed to prevent falsification information.
Irine, I. (Irine) +2 more
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