Results 141 to 150 of about 4,713,939 (389)
Preparation of Mealybugs (Hemiptera: Pseudococcidae) for Genetic Characterization and Morphological Examination. [PDF]
Mealybugs (Hemiptera: Pseudococcidae) are economically significant agricultural pests on many different crops. Because of their small size and lack of easily visible characters for identification, determination of their taxonomic status is difficult and ...
Bahder, BW+3 more
core +1 more source
A mostly automated protocol for extraction of genomic DNA from seawater filtered onto a Sterivex filter (Cat. No. SVGP0150). Reagents come from the Macherey-Nagel NucleoMag Plant Kit for DNA purfication (Cat. No. 744400). Automated liquid handling is performed on an eppendorf EpMotion 5075t with multi-channel pipettes.
Rob Lampe, Andrew E Allen
openaire +2 more sources
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka+10 more
wiley +1 more source
Simplified DNA Extraction Methods for Sexing Chick Embryos
We investigated the efficiency of DNA extraction and the accuracy of polymerase chain reaction- (PCR-) based sexing of DNA that was obtained from adult chickens and early chicken embryos. DNA samples were prepared using water-, ammonium-, and proteinase-
Takeo Minematsu+4 more
doaj +1 more source
Characteristics of Alveolar Bone Marrow Cells from Patients Undergoing Dental Extractions or Dental Implant Therapy [PDF]
Alveolar bone marrow stromal cells (aBMSCs) play important roles in craniofacial wound healing. To establish an easy, efficient and reliable method to harvest aBMSCs, we compared three different methods: extraction socket aspiration, osteotomy aspiration
Lee, Meng H
core +2 more sources
Ekstraksi Dna Dan Amplifikasi Its Rdna Isolat Fungi Endofit Lbkurcc67 Umbi Tanaman Dahlia (Dahlia Variabilis) [PDF]
> Endophyte fungi lives in the plant tissues without causing harm to their host and has known that can produce secondary metabolite and extracellular enzyme.
Charles H. Lang (318203)+5 more
core +3 more sources
Integrating ancestry, differential methylation analysis, and machine learning, we identified robust epigenetic signature genes (ESGs) and Core‐ESGs in Black and White women with endometrial cancer. Core‐ESGs (namely APOBEC1 and PLEKHG5) methylation levels were significantly associated with survival, with tumors from high African ancestry (THA) showing ...
Huma Asif, J. Julie Kim
wiley +1 more source
The Case for National DNA Identification Cards [PDF]
Foes of the United States have demonstrated their ability to strike at the heart of this country. Fear of renewed attacks and a desire for greater national security have now prompted many to call for improvements in the national personal identification ...
Quarmby, Ben
core +1 more source
Insect collections and DNA analyses: how to manage collections? [PDF]
Numerous papers have been recently published regarding DNA studies of museum insect specimens. Despite the great enthusiasm for such analyses, there continues to be a potential conflict between specimen maintenance and their use for experimental ...
MANDRIOLI, Mauro
core +1 more source
Efficient and easy-to-use DNA extraction and purification methods are critical in implementing PCR-based diagnosis of pathogens. In order to optimize the routine clinical laboratory diagnosis of eukaryotic enteric pathogens, we compare, via quantitative ...
E. Menu+5 more
semanticscholar +1 more source