Results 151 to 160 of about 4,748,303 (367)

Monitoring genetic population biomarkers for wastewater-based epidemiology [PDF]

open access: yes, 2017
We report a rapid “sample-to-answer” platform that can be used for the quantitative monitoring of genetic biomarkers within communities through the analysis of wastewater. The assay is based on the loop-mediated isothermal amplification (LAMP) of nucleic
Cooper, Jonathan M.   +4 more
core   +3 more sources

Equation of state of looped DNA [PDF]

open access: yesarXiv, 2005
We calculate the equation of state of DNA under tension for the case that the DNA features loops. Such loops occur transiently during DNA condensation in the presence of multivalent ions or sliding cationic protein linkers. The force-extension relation of such looped DNA modelled as a wormlike chain is calculated via path integration in the ...
arxiv  

Comparison of DNA extraction methods for non-marine molluscs: is modified CTAB DNA extraction method more efficient than DNA extraction kits?

open access: yesbioRxiv, 2019
Isolation of high molecular weight DNA from gastropod molluscs and its subsequent PCR amplification is considered difficult due to excessive mucopolysaccharides secretion which co-precipitate with DNA and obstruct successful amplification.
Sudeshna Chakraborty   +2 more
semanticscholar   +1 more source

Circulating tumor DNA (ctDNA) trajectories predict survival in trifluridine/tipiracil‐treated metastatic colorectal cancer patients

open access: yesMolecular Oncology, EarlyView.
The authors applied joint/mixed models that predict mortality of trifluridine/tipiracil‐treated metastatic colorectal cancer patients based on circulating tumor DNA (ctDNA) trajectories. Patients at high risk of death could be spared aggressive therapy with the prospect of a higher quality of life in their remaining lifetime, whereas patients with a ...
Matthias Unseld   +7 more
wiley   +1 more source

Optimization of DNA extraction from seeds and fresh leaf tissues of Soybean (Glycine max) [PDF]

open access: yes, 2012
The effects of various components on extraction buffer such as SDS-NaCI, PVP, p-mercaptoethanol, extraction with Phenol:Chloroform:lsoamyl acetate, and incubation time on the DNA extraction from seeds and fresh leaves of Soybean (Glycine max)were ...
., Evellyn   +2 more
core  

Integrative systems‐level analysis reveals a contextual crosstalk between hypoxia and global metabolism in human breast tumors

open access: yesMolecular Oncology, EarlyView.
Breast tumor samples scored for metabolic deregulation (M1 to M3) were given a hypoxia score (HS). The highest HS occurred in patients with strongest metabolic deregulation (M3), supporting tumor aggressiveness. HS correlated with the highest number of metabolic pathways in M1. This suggests hypoxia to be an early event in metabolic deregulation.
Raefa Abou Khouzam   +2 more
wiley   +1 more source

Low concentration DNA extraction and recovery using a silica solid phase

open access: yesPLoS ONE, 2017
DNA extraction from clinical samples is commonly achieved with a silica solid phase extraction column in the presence of a chaotrope. Versions of these protocols have been adapted for point of care (POC) diagnostic devices in miniaturized platforms, but ...
Constantinos Katevatis   +2 more
semanticscholar   +1 more source

Crosstalk between gut microbiota and tumor: tumors could cause gut dysbiosis and metabolic imbalance

open access: yesMolecular Oncology, EarlyView.
In this research, we analyzed the relationship between gut microbiota and tumor. We discovered that both subcutaneous and metastatic tumors would alter the composition and metabolic function of gut microbiota. Meanwhile, fecal microbiota transplantation also indicated the anti‐tumor role of the gut microbiota, revealing the crosstalk between tumor and ...
Siyuan Zhang   +8 more
wiley   +1 more source

Genomic Dna Extraction Method From Mature Leaf Of Lai (Durio Kutejensis Becc.) [PDF]

open access: yes, 2016
Lai (Durio kutejensis Becc.) is an indigeneous germplasm of Kalimantan which has some superiorities compared to its close-relative, durian (Durio zibethinus Murr.). Genetic exploration of lai is important to support its breeding program.
Handayani, F. (Fitri)   +2 more
core   +2 more sources

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