Results 161 to 170 of about 4,713,939 (389)

A crude method of DNA extraction and identification from exfoliated human buccal mucosa cells

Indian Journal of Dental Research, 2019
Background: DNA analysis has a key role in forensic dentistry. However, techniques of DNA extraction and analysis are far from the reach of majority of medical professionals owing to its expensive set up. Aim: The present study was aimed at formulating a
Priyanka Kardam, Monica Mehendiratta, Shweta Rehani, Rashi Sharma, Khushboo Sahay   +4 more
doaj   +1 more source

A rapid and low-cost DNA extraction method for isolating Escherichia coli DNA from animal stools [PDF]

, 2011
The price of commercial DNA extraction methods makes the routine use of polymerase chain reaction amplification (PCR) based methods rather costly for scientists in developing countries. A guanidium thiocayante-based DNA extraction method was investigated
Barnard, T. G., Jagals, P., Potgieter, N., Robertson, C. A.   +3 more
core  

Low concentration DNA extraction and recovery using a silica solid phase

PLoS ONE, 2017
DNA extraction from clinical samples is commonly achieved with a silica solid phase extraction column in the presence of a chaotrope. Versions of these protocols have been adapted for point of care (POC) diagnostic devices in miniaturized platforms, but ...
Constantinos Katevatis, Andy Fan, C. Klapperich   +2 more
semanticscholar   +1 more source

Plasmid DNA extraction v2

, 2021
This protocol is used to extract plasmid DNA from E. coli.
openaire   +2 more sources

Multi‐omic characterization of consensus molecular subtype 1 (CMS1) colorectal cancer with dampened immune response improves precision medicine

Molecular Oncology, EarlyView.
This study highlights the importance of multi‐omic analyses in characterizing colorectal cancers. Indeed, our analysis revealed a rare CMS1 exhibiting dampened immune activation, including reduced PD‐1 expression, moderate CD8+ T‐cell infiltration, and suppressed JAK/STAT pathway.
Livia Concetti, Manuel Scimeca, Julia Bischof, Jonathan Woodsmith, Massimiliano Agostini, Cristina Fiorani, Yufang Shi, Eleonora Candi, Gerry Melino, Alessandro Mauriello, Giuseppe S. Sica   +10 more
wiley   +1 more source

Whole Genome Sequencing of A Healthy Sundanese Individual As an Initial Study for Sundanese Population Genetic Reference Foundation [PDF]

BIO Web of Conferences
Sundanese is the second-largest tribe in Indonesia. Despite its substantial and diverse population, the application and database of whole-genome sequencing in Indonesia, especially among Sundanese individuals, are limited.
Jamilah Richa Wijayanti, Andrianto Dimas, Pratama Rahadian, Budhyanto Vincentius Simeon Weo, Pudjas Nabila Tsoerayya Gustia   +4 more
doaj   +1 more source

The Effect of DNA Extraction Methods on Observed Microbial Communities from Fibrous and Liquid Rumen Fractions of Dairy Cows

Frontiers in Microbiology, 2018
DNA based methods have been widely used to study the complexity of the rumen microbiota, and it is well known that the method of DNA extraction is a critical step in enabling accurate assessment of this complexity.
J. D. Vaidya, B. van den Bogert, J. Edwards, J. Boekhorst, Sanne van Gastelen, E. Saccenti, C. Plugge, H. Smidt   +7 more
semanticscholar   +1 more source

A DNA extraction method that allows reliable PCR amplification of MLO DNA from "difficult" plant host species. [PDF]

, 1994
Karen Gibb, Anna Padovan
openalex   +1 more source

Targeting the AKT/mTOR pathway attenuates the metastatic potential of colorectal carcinoma circulating tumor cells in a murine xenotransplantation model

Molecular Oncology, EarlyView.
Dual targeting of AKT and mTOR using MK2206 and RAD001 reduces tumor burden in an intracardiac colon cancer circulating tumor cell xenotransplantation model. Analysis of AKT isoform‐specific knockdowns in CTC‐MCC‐41 reveals differentially regulated proteins and phospho‐proteins by liquid chromatography coupled mass spectrometry. Circulating tumor cells
Daniel J. Smit, Thais Pereira‐Veiga, Helena Brauer, Michael Horn, Paula Nissen, Thomas Mair, Bente Siebels, Hannah Voß, Ruimeng Zhuang, Marie‐Therese Haider, Desiree Loreth, Margarita Iskhakova, Bele Lindemann, Julian Kött, Laure Cayrefourcq, Jasmin Wellbrock, Hartmut Schlüter, Klaus Pantel, Catherine Alix‐Panabières, Manfred Jücker   +19 more
wiley   +1 more source

A simple method for extraction of DNA from Guthrie cards. [PDF]

, 1992
Christian Schneeberger, F Kury, JWA Larsen, P Speiser, Robert Zeillinger   +4 more
openalex   +1 more source