Results 161 to 170 of about 4,873,130 (388)

Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels

Frontiers in Marine Science, 2017
Metabarcoding of marine environmental DNA (eDNA), the DNA suspended in seawater originating from tissue, cells, or extracellular DNA, offers the opportunity to survey the biological composition of communities across multiple trophic levels from a non ...
Anni Djurhuus, Jesse A. Port, Collin J. Closek, K. Yamahara, Ofelia C Romero-Maraccini, K. Walz, Dawn B. Goldsmith, R. Michisaki, M. Breitbart, A. Boehm, F. Chavez   +10 more
semanticscholar   +1 more source

Integrated genomic and proteomic profiling reveals insights into chemoradiation resistance in cervical cancer

Molecular Oncology, EarlyView.
A comprehensive genomic and proteomic analysis of cervical cancer revealed STK11 and STX3 as a potential biomarkers of chemoradiation resistance. Our study demonstrated EGFR as a therapeutic target, paving the way for precision strategies to overcome treatment failure and the DNA repair pathway as a critical mechanism of resistance.
Janani Sambath, Irene A. George, Srikanth S. Manda, Prasanth Ariyannur, Ekta R. Dhawale, Raja Sekhar Kommu, Rajan Datar, Darshana Patil, Vinita Trivedi, Manisha Singh, Kumar Prabhash, Sewanti Limaye, Richa Chauhan, Prashant Kumar   +13 more
wiley   +1 more source

Preliminary validation of Prepfiler Express™ Extraction kit in AutoMate Express DNA Extraction System [PDF]

, 2011
F. Balsa, V. Bogas, Pricila da Silva Cunha, P. Brito, A. Serra, V. Lopes, M. Carvalho, L.H.C. Andrade, A.M. Bento, M. São Bento, Francisco Gonçalves, M.J. Anjos   +11 more
openalex   +1 more source

An Analysis of Procedural and Conceptual Knowledge in Genetic Engineering Topic among STEM Students: Basis for Instructional Material Development

Bioeduca
The objective of this study is to examine the levels of procedural and conceptual knowledge in genetic engineering topics among STEM students to provide baseline in developing effective instructional materials.
Anwar I. Kolong, Melbert C. Sepe
doaj   +1 more source

Performance comparison of three DNA extraction kits on human whole-exome data from formalin-fixed paraffin-embedded normal and tumor samples

PLoS ONE, 2018
Next-generation sequencing (NGS) studies are becoming routinely used for the detection of novel and clinically actionable DNA variants at a pangenomic scale. Such analyses are now used in the clinical practice to enable precision medicine. Formalin-fixed
E. Bonnet, Marie-Laure Moutet, Céline Baulard, D. Bacq-Daian, Florian Sandron, L. Mesrob, B. Fin, M. Delepine, M. Palomares, Claire Jubin, H. Blanché, V. Meyer, A. Boland, R. Olaso, J. Deleuze   +14 more
semanticscholar   +1 more source

Gut microbiota diversity is prognostic in metastatic hormone receptor‐positive breast cancer patients receiving chemotherapy and immunotherapy

Molecular Oncology, EarlyView.
In this exploratory study, we investigated the relationship between the gut microbiota and outcome in patients with metastatic hormone receptor‐positive breast cancer, treated in a randomized clinical trial with chemotherapy alone or chemotherapy in combination with immune checkpoint blockade.
Andreas Ullern, Kristian Holm, Nikolai Kragøe Andresen, Andreas Hagen Røssevold, Corinna Bang, Bjørn Naume, Johannes R. Hov, Jon Amund Kyte   +7 more
wiley   +1 more source

The choice of the DNA extraction method may influence the outcome of the soil microbial community structure analysis

MicrobiologyOpen, 2017
Metagenomics approaches and recent improvements in the next‐generation sequencing methods, have become a method of choice in establishing a microbial population structure. Many commercial soil DNA extraction kits are available and due to their efficiency
S. Zielińska, P. Radkowski, Aleksandra Blendowska, A. Ludwig-Gałęzowska, J. Łoś, M. Łoś   +5 more
semanticscholar   +1 more source

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

A simple plant high-molecular-weight DNA extraction method suitable for single-molecule technologies [PDF]

, 2020
Zhigang Li, Stephen Parris, Christopher Saski   +2 more
openalex   +1 more source

PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism

Molecular Oncology, EarlyView.
This study investigated how PYCR1 inhibition in bone marrow stromal cells (BMSCs) indirectly affects multiple myeloma (MM) cell metabolism and viability. Culturing MM cells in conditioned medium from PYCR1‐silenced BMSCs impaired oxidative phosphorylation and increased sensitivity to bortezomib.
Inge Oudaert, Lauren van den Broecke, Osman Aksoy, Judith Lind, Sonia Vallet, Arne Van der Vreken, Gamze Ates, Ann Massie, Ken Maes, Kim De Veirman, Elke De Bruyne, Karin Vanderkerken, Klaus Podar, Eline Menu   +13 more
wiley   +1 more source