Results 281 to 290 of about 4,873,130 (388)

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

DNA-extraction of Daphnia and symbionts v1 [PDF]

Pascal Angst, Peter D. Fields
openalex   +1 more source

Laboratory diagnostics of common bunt and dwarf bunt

Czech Journal of Genetics and Plant Breeding, 2006
M. Kochanová, E. Prokinová, P. Ryšánek   +2 more
doaj   +1 more source

Evaluation of four DNA extraction kits for implementation of nanopore sequencing in routine surveillance of antimicrobial resistance in low-resource settings. [PDF]

Front Microbiol
Thornval NR, Lacy-Roberts N, Nilsson P, Espinosa-Gongora C, Hasman H, Mourão J, Rasmussen A, Rebelo AR, Gibson C, Hendriksen RS.   +9 more
europepmc   +1 more source

Evolutionary dynamics of the chloroplast genome in Daphne (Thymelaeaceae): comparative analysis with related genera and insights into phylogenetics

FEBS Open Bio, EarlyView.
Comparative analysis of chloroplast genomes from 14 genera of Thymelaeaceae revealed variation in gene content, ranging from 128 to 142 genes, primarily influenced by IR expansion/contraction events and pseudogenization of ndhF, ndhI, and ndhG. Two large inversions were detected within the large single‐copy region, including a synapomorphic inversion ...
Abdullah, Hui Li, Rushan Yan, Chengyu Chen, Madiha Islam, Abdul Majid, Ibrar Ahmed, Parviz Heidari, Xiaoxuan Tian   +8 more
wiley   +1 more source

Successful optical genome mapping from 500 000 cells: A low-input UHMW DNA extraction approach. [PDF]

MethodsX
De Vlieghere E, Nollet F, Devos H, Cauwelier B.   +3 more
europepmc   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Optimization of DNA extraction methods from pig farm wastewater for pathogen detection using metagenomic sequencing. [PDF]

Microb Genom
Muralidhar M, Naseem MN, Mesa JRB, Omaleki L, Turni C.   +4 more
europepmc   +1 more source

A comparison of DNA extraction methods for high‐throughput DNA analyses

Molecular Ecology Resources, 2017
L. M. Schiebelhut, Sarah S. Abboud, L. Gómez Daglio, Holly F. Swift, M. Dawson   +4 more
semanticscholar   +1 more source

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

FEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source