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Caulimovirus Isolation and DNA Extraction
1998Members of the cauhmovn-us group (1) each have a circular double-stranded DNA genome of approx 8 kbp that is encapsidated m a spherical, naked nucleocapsid of approx 50 nm diameter (Fig. 1). Caulimovnuses charactertsttcally produce subcellular mclusron bodies m infected tissues that contam most of the virions found in cells, embedded m an apparently ...
Nadia S. Al-Kaff +3 more
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Extraction/Characterization of DNA
2012Abstract Some of the foundational procedures of modern biology are the extraction and characterization of DNA from various cultures and tissues. This chapter guides the reader through the key principles of DNA extraction using some of the most commonly needed procedures, including preparation of (1) plasmid DNA from bacterial cultures; (2) plant ...
Leland J. Cseke, Joseph R. Herdy
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Extraction of DNA from Paleofeces
2011Paleofeces are the nonmineralized remains of dung from extant and extinct fauna. They represent a surprisingly large proportion of fossil remains recovered from cave sites across the world. Paleofeces contain the DNA of the defecator as well as the DNA of ingested plant and animal remains.
Melanie Kuch, Hendrik N. Poinar
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Phytoplasma Plasmid DNA Extraction
2012Phytoplasma plasmids have generally been detected from DNA extracted from plants and insects using methods designed for the purification of total phytoplasma DNA. Methods include extraction from tissues that are high in phytoplasma titre, such as the phloem of plants, with the use of CsCl-bisbenzimide gradients that exploit the low G+C content of ...
Mark T. Andersen, Lia W. Liefting
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2001
The extraction of DNA from plants is the starting point for genotype analysis. The approach to preparation of DNA from plants is determined by the species, the type of tissue or sample available and the analysis required on the DNA. For example, extraction of DNA from the leaf of a cereal for analysis using a robust test may require a very different ...
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The extraction of DNA from plants is the starting point for genotype analysis. The approach to preparation of DNA from plants is determined by the species, the type of tissue or sample available and the analysis required on the DNA. For example, extraction of DNA from the leaf of a cereal for analysis using a robust test may require a very different ...
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2010
Publisher Summary This chapter focuses on the DNA extraction process. DNA molecules must be separated from other cellular material before they can be examined. Cellular proteins that package and protect DNA in the environment of the cell can inhibit the ability to analyze the DNA.
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Publisher Summary This chapter focuses on the DNA extraction process. DNA molecules must be separated from other cellular material before they can be examined. Cellular proteins that package and protect DNA in the environment of the cell can inhibit the ability to analyze the DNA.
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2008
The isolation of genomic DNA is a fundamental requirement for many analytical molecular biology procedures. Although proper collection and stabilisation of the sample is crucial, purification of the DNA is often the key step for success in downstream analysis.
Ginny C. Saunders, Jennifer M. Rossi
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The isolation of genomic DNA is a fundamental requirement for many analytical molecular biology procedures. Although proper collection and stabilisation of the sample is crucial, purification of the DNA is often the key step for success in downstream analysis.
Ginny C. Saunders, Jennifer M. Rossi
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Extraction and Precipitation of
AbstractThis appendix presents protocols for phenol extraction and ethanol precipitation, methods that are routinely used to isolate DNA from biological sources or enzymatic reaction mixtures, to concentrate DNA samples, and to change from one solvent system to another.
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DNA Extraction from Fossil Eggshell
2011Avian eggshell fragments recovered from both paleontological and archaeological deposits contain a cache of well-preserved ancient DNA. Here, we describe an extraction protocol that has been optimized to maximize the recovery of ancient DNA from fossil eggshell and minimize the co-purification of PCR inhibitors.
Oskam, C.L., Bunce, M.
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DNA extraction from actinorhizal nodules
1996The nitrogen-fixing actinomycete Frankia has its main ecological niche in the root nodules that are formed with a wide taxonomic range of host plants, called by this fact actinorhizal plants. This generic name comprises more than 200 species of vascular dicotyledonous plants, distributed in 20 genera and eight families [5].
Ramirez-Saad, H. +2 more
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