Results 11 to 20 of about 18,298 (211)
iScience, 2022 Summary: Gene silencing in bacteria is mediated by chromatin proteins, of which Escherichia coli H-NS is a paradigmatic example. H-NS forms nucleoprotein filaments with either one or two DNA duplexes.
Beth A. Shen +4 more
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Footprinting with an Automated Capillary DNA Sequencer
BioTechniques, 2000 Footprinting is a valuable tool for studying DNA-protein contacts. However, it usually involves expensive, tedious and hazardous steps such as radioactive labeling and analyses on polyacrylamide sequencing gels.
Wandee Yindeeyoungyeon, Mark A. Schell
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BioTechniques, 2003 Identification of nucleotides used for RNA chain initiation or for contacting DNA binding proteins is basic to our understanding of gene regulation. Normally, a radioactive primer is used to copy RNA or DNA. The polymerase extension stops at free ends of
Richard A. Fekete +2 more
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DNase I Footprinting to Identify Protein Binding Sites
Bio-Protocol, 2013 DNase I footprinting is used to precisely localise the position that a DNA binding protein, e.g. a transcription factor, binds to a DNA fragment. A DNA fragment of a few hundred bp is labelled at one end and then incubated with the proteins suspected to ...
Isabelle Gaugué +2 more
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HIV-1 Integrase-DNA Recognition Mechanisms
Viruses, 2009 Integration of a reverse transcribed DNA copy of the HIV viral genome into the host chromosome is essential for virus replication. This process is catalyzed by the virally encoded protein integrase. The catalytic activities, which involve DNA cutting and
Mamuka Kvaratskhelia +5 more
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Targeted Downregulation of MYC through G-quadruplex Stabilization by DNAi
Molecules, 2021 Modulating the expression or function of the enigmatic MYC protein has demonstrated efficacy in an array of cancer types and a marked potential therapeutic index and safety profile. Despite its high therapeutic value, specific and selective inhibitors or
Alexandra Maria Psaras +3 more
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Nature Communications, 2023 Decades ago, it was shown that proteins binding to DNA can quantitatively alter the formation of DNA damage by UV light. This established the principle of UV footprinting for non-intrusive study of protein-DNA contacts in living cells, albeit at limited ...
Kerryn Elliott +3 more
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Computational and Structural Biotechnology Journal, 2022 The BvgAS two-component system regulates virulence gene expression in Bordetella pertussis. Although precise three-dimensional structural information is not available for the response regulator BvgA, its sequence conservation with E.
David Kim +12 more
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Attachment of a 32P-phosphate to the 3′ Terminus of a DNA Oligonucleotide
Bio-Protocol, 2020 Biochemical investigations into DNA-binding and DNA-cutting proteins often benefit from the specific attachment of a radioactive label to one of the two DNA termini.
Joshua Cofsky, Jennifer Doudna
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Characterization of Dnmt1 Binding and DNA Methylation on Nucleosomes and Nucleosomal Arrays. [PDF]
PLoS ONE, 2015 The packaging of DNA into nucleosomes and the organisation into higher order structures of chromatin limits the access of sequence specific DNA binding factors to DNA.
Anna Schrader +3 more
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