Results 21 to 30 of about 18,298 (211)

Cleavage of phosphorothioated DNA and methylated DNA by the type IV restriction endonuclease ScoMcrA. [PDF]

PLoS Genetics, 2010
Many taxonomically diverse prokaryotes enzymatically modify their DNA by replacing a non-bridging oxygen with a sulfur atom at specific sequences. The biological implications of this DNA S-modification (phosphorothioation) were unknown.
Guang Liu, Hong-Yu Ou, Tao Wang, Li Li, Huarong Tan, Xiufen Zhou, Kumar Rajakumar, Zixin Deng, Xinyi He   +8 more
doaj   +1 more source

How an essential Zn2Cys6 transcription factor PoxCxrA regulates cellulase gene expression in ascomycete fungi?

Biotechnology for Biofuels, 2019
Background Soil ascomycete fungi produce plant-biomass-degrading enzymes to facilitate nutrient and energy uptake in response to exogenous stress. This is controlled by a complex signal network, but the regulatory mechanisms are poorly understood.
Lu-Sheng Liao, Cheng-Xi Li, Feng-Fei Zhang, Yu-Si Yan, Xue-Mei Luo, Shuai Zhao, Jia-Xun Feng   +6 more
doaj   +1 more source

Binding of NF-κB to nucleosomes: effect of translational positioning, nucleosome remodeling and linker histone H1. [PDF]

PLoS Genetics, 2013
NF-κB is a key transcription factor regulating the expression of inflammatory responsive genes. How NF-κB binds to naked DNA templates is well documented, but how it interacts with chromatin is far from being clear. Here we used a combination of UV laser
Imtiaz Nisar Lone, Manu Shubhdarshan Shukla, John Lalith Charles Richard, Zahary Yordanov Peshev, Stefan Dimitrov, Dimitar Angelov   +5 more
doaj   +1 more source

Protein-DNA associations in a gender-specific gene of Schistosoma mansoni: characterization by UV cross-linking, DNase I footprinting and band shift assays

Memorias do Instituto Oswaldo Cruz, 1992
Protein extracts obtained from male and female shistosomes were incubated with a gender-specific gene, F-10, transcribed only in adult females and encoding a major egg-shell protein.
Simone Engelender, Franklin D. Rumjanek
doaj   +1 more source

Multidomain architecture of estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains

Nature Communications, 2018
The human estrogen receptor alpha (hERα) is a hormone-responsive transcription factor. Here the authors combine small-angle X-ray scattering, hydroxyl radical protein footprinting and computational modeling and show that multidomain hERα adopts an L ...
Wei Huang, Yi Peng, Janna Kiselar, Xuan Zhao, Aljawharah Albaqami, Daniel Mendez, Yinghua Chen, Srinivas Chakravarthy, Sayan Gupta, Corie Ralston, Hung-Ying Kao, Mark R. Chance, Sichun Yang   +12 more
doaj   +1 more source

Amidst multiple binding orientations on fork DNA, Saccharolobus MCM helicase proceeds N-first for unwinding

eLife, 2019
DNA replication requires that the duplex genomic DNA strands be separated; a function that is implemented by ring-shaped hexameric helicases in all Domains.
Himasha M Perera, Michael A Trakselis
doaj   +1 more source

Nucleosome footprinting in plasma cell-free DNA for the pre-surgical diagnosis of ovarian cancer

npj Genomic Medicine, 2022
Fragmentation patterns of plasma cell-free DNA (cfDNA) are known to reflect nucleosome positions of cell types contributing to cfDNA. Based on cfDNA fragmentation patterns, the deviation in nucleosome footprints was quantified between diagnosed ovarian ...
Adriaan Vanderstichele, Pieter Busschaert, Chiara Landolfo, Siel Olbrecht, An Coosemans, Wouter Froyman, Liselore Loverix, Nicole Concin, Elena Ioana Braicu, Pauline Wimberger, Els Van Nieuwenhuysen, Sileny N. Han, Toon Van Gorp, Tom Venken, Ruben Heremans, Patrick Neven, Tom Bourne, Ben Van Calster, Dirk Timmerman, Diether Lambrechts, Ignace Vergote   +20 more
doaj   +1 more source

Bivariate Genomic Footprinting Detects Changes in Transcription Factor Activity

Cell Reports, 2017
In response to activating signals, transcription factors (TFs) bind DNA and regulate gene expression. TF binding can be measured by protection of the bound sequence from DNase digestion (i.e., footprint).
Songjoon Baek, Ido Goldstein, Gordon L. Hager   +2 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

2,3-Bifunctionalized Quinoxalines: Synthesis, DNA Interactions and Evaluation of Anticancer, Anti-tuberculosis and Antifungal Activity

Molecules, 2002
A variety of 2,3-bifunctionalized quinoxalines (6-14) have been prepared by the condensation of 1,6-disubstituted-hexan-1,3,4,6-tetraones (1-4) with o-phenylenediamine, (R,R)-1,2-diaminocyclohexane and p-nitro-o-phenylenediamine.
Tom Ellis, Mohamed Bouakka, Abdelkader Hakkou, Sghir Elkadiri, Rachid Touzani, Abdelkrim Ramdani, Ann T. Kotchevar, Taibi Ben-Hadda, Michael J. Waring   +8 more
doaj   +1 more source