Results 11 to 20 of about 373,852 (297)
DNA fragmentation in apoptosis [PDF]
Cell Research, 2000 Cleavage of chromosomal DNA into oligonucleosomal size fragments is an integral part of apoptosis. Elegant biochemical work identified the DNA fragmentation factor (DFF) as a major apoptotic endonuclease for DNA fragmentation in vitro. Genetic studies in mice support the importance of DFF in DNA fragmentation and possibly in apoptosis in vivo.
J H, Zhang, M, Xu
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Asian Journal of Andrology, 2022 We question whether, in men with an abnormal rate of sperm DNA fragmentation, the magnetic-activated cell sorting (MACS) could select spermatozoa with lower rates of DNA fragmentation as well as spermatozoa with unbalanced chromosome content ...
Sahar El Fekih +9 more
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Electroeluting DNA Fragments [PDF]
Journal of Visualized Experiments, 2010 Purified DNA fragments are used for different purposes in Molecular Biology and they can be prepared by several procedures. Most of them require a previous electrophoresis of the DNA fragments in order to separate the band of interest. Then, this band is excised out from an agarose or acrylamide gel and purified by using either: binding and elution ...
Zarzosa-Álvarez, Ana L. +3 more
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Translational Andrology and Urology, 2017 I agree with Agarwal et al. (1) that sperm DNA fragmentation (SDF) should be included in the evaluation of male factor infertility, along with semen analysis.
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Comparison between density gradient centrifugation method, an extended version of the horizontal swim up method and the combination of both for sperm selection [PDF]
Obstetrics & Gynecology Science, 2023 Objective To compare the degree of efficiency between density gradient centrifugation (DGC) method and an extended horizontal swim-up (SU) method. Methods A total of 97 couples undergoing in vitro fertilization were enrolled in the study.
Malak Jamil +12 more
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Critical parameters for efficient sonication and improved chromatin immunoprecipitation of high molecular weight proteins [PDF]
, 2016 Solubilization of cross-linked cells followed by chromatin shearing is essential for successful chromatin immunoprecipitation (ChIP). However, this task, typically accomplished by ultrasound treatment, may often become a pitfall of the process, due to ...
Adams, Peter D. +2 more
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PLoS ONE, 2021 Poor sperm morphology and an elevated DNA fragmentation level are considered to be related to spermiogenesis malfunctions as a result of genetic mutations and effects of environmental factors, including industrial pollution.
Maxim Kleshchev +2 more
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Understanding sperm DNA fragmentation [PDF]
Translational Andrology and Urology, 2017 We greatly value the commentary by Dr. Gosalvez on the “Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios” by Agarwal et al. (1). The author initiated his well-written note with an overview of the structural and physiologic significance of sperm DNA on male reproduction addressing its ...
Majzoub, Ahmad +2 more
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DNA Fragment Conformations [PDF]
European Journal of Biochemistry, 1982 The helix‐coil transition, conformations and dynamic structures of d(A‐C‐A‐T‐G‐T) we re investigated as a function of temperature by 1H‐NMR spectroscopy at various frequencies (400, 276, 250 arid 90 MHz). Imino exchangeable protons of the d(A‐C‐A‐T‐G‐T) duplex were observed between 5°C and 25°C at 276 MHz.
Jean‐Michel NEUMANN +5 more
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Fertilization capacity with rainbow trout DNA-damaged sperm and embryo developmental success [PDF]
, 2010 Mammalian spermatozoa undergo a strong selection process along the female tract to guarantee fertilization by good quality cells, but risks of fertilization with DNA-damaged spermatozoa have been reported.
Ahmadi +31 more
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