Results 1 to 10 of about 204,474 (261)

DNA fragmentation in apoptosis [PDF]

open access: yesCell Research, 2000
Cleavage of chromosomal DNA into oligonucleosomal size fragments is an integral part of apoptosis. Elegant biochemical work identified the DNA fragmentation factor (DFF) as a major apoptotic endonuclease for DNA fragmentation in vitro. Genetic studies in mice support the importance of DFF in DNA fragmentation and possibly in apoptosis in vivo.
Ming Xu, John H Zhang
exaly   +3 more sources

Oxidative origin of sperm DNA fragmentation in the adult varicocele [PDF]

open access: yesInternational Brazilian Journal of Urology, 2021
Purpose: Sperm DNA fragmentation is a major cellular mechanism underlying varicocele-related male infertility. However, the type of DNA fragmentation - whether oxidative or of another nature - remains unknown.
Jessica Timóteo Jeremias   +6 more
doaj   +1 more source

Whether the Effect of Sperm DNA Fragmentation on the Clinical and Laboratory Outcomes Changes with the Paternal age in the intracytoplasmic sperm injection Cycles of Donor Oocytes

open access: yesGynecology Obstetrics & Reproductive Medicine, 2022
OBJECTIVE: The present study aimed to know the effect of paternal age together with Sperm DNA fragmentation on the clinical and laboratory outcomes in the donor oocyte intracytoplasmic sperm injection cycles.
Deepthi Repalle   +3 more
doaj   +1 more source

Sperm DNA Fragmentation: Causes, Effects and Relevance in IVF Outcomes

open access: yesFertility & Reproduction, 2023
Sperm DNA fragmentation, characterized by damaged or broken DNA strands within sperm cells, has been recognized as an important factor in male infertility and poor ART outcome.
David YL Chan
doaj   +1 more source

Evaluation of Sperm DNA Fragmentation Using Two Different Methods: TUNEL via Fluorescence Microscopy, and Flow Cytometry

open access: yesMedicina, 2023
Background and Objectives: Sperm DNA fragmentation refers to any break in one or both of the strands of DNA in the head of a sperm. The most widely used methodologies for assessing sperm DNA fragmentation are the sperm chromatin structure assay (SCSA ...
Katerina Chatzimeletiou   +8 more
doaj   +1 more source

MACS-annexin V cell sorting of semen samples with high TUNEL values decreases the concentration of cells with abnormal chromosomal content: a pilot study

open access: yesAsian Journal of Andrology, 2022
We question whether, in men with an abnormal rate of sperm DNA fragmentation, the magnetic-activated cell sorting (MACS) could select spermatozoa with lower rates of DNA fragmentation as well as spermatozoa with unbalanced chromosome content ...
Sahar El Fekih   +9 more
doaj   +1 more source

SPERM DNA FRAGMENTATION [PDF]

open access: yesArchives of Andrology, 2006
Several techniques have been developed to measure the amount of sperm DNA damage in an effort to identify more objective parameters for evaluation of infertile men. The integrity of sperm DNA influences a couple's fertility and helps predict the chances of pregnancy and its successful outcome.
A, Shafik   +3 more
openaire   +2 more sources

Sperm DNA fragmentation [PDF]

open access: yesTranslational Andrology and Urology, 2017
I agree with Agarwal et al. (1) that sperm DNA fragmentation (SDF) should be included in the evaluation of male factor infertility, along with semen analysis.
openaire   +5 more sources

Electroeluting DNA Fragments [PDF]

open access: yesJournal of Visualized Experiments, 2010
Purified DNA fragments are used for different purposes in Molecular Biology and they can be prepared by several procedures. Most of them require a previous electrophoresis of the DNA fragments in order to separate the band of interest. Then, this band is excised out from an agarose or acrylamide gel and purified by using either: binding and elution ...
Zarzosa-Álvarez, Ana L.   +3 more
openaire   +2 more sources

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