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Isolation of DNA Fragments for Microinjection
2003The purification of a DNA fragment for microinjection is extremely important. This chapter describes a rapid and efficient technique for isolating specific DNA fragments from agarose gels run in Tris-acetate buffer, and was first described by Vogelstein and Gillespie.
Smith, D.R., Murphy, D.
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Current Protocols in Molecular Biology, 1991
AbstractThe essence of recombinant DNA technology is the joining of two or more separate segments of DNA to generate a single DNA molecule that is capable of autonomous replication in a given host. The simplest constructions of hybrid DNA molecules involve the cloning of insert sequences into plasmid or bacteriophage cloning vectors.
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AbstractThe essence of recombinant DNA technology is the joining of two or more separate segments of DNA to generate a single DNA molecule that is capable of autonomous replication in a given host. The simplest constructions of hybrid DNA molecules involve the cloning of insert sequences into plasmid or bacteriophage cloning vectors.
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Measurement of Apoptosis by DNA Fragmentation
2004Classical apoptotic cell death can be defined by certain morphological and biochemical characteristics that distinguish it from other forms of cell death. One such feature, which is a hallmark of apoptosis, is DNA fragmentation. In dying cells, DNA is cleaved by an endonuclease that fragments the chromatin into nucleosomal units, which are multiples of
Demetrius, Matassov +4 more
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Nucleosomal DNA Fragments in Autoimmune Diseases
Annals of the New York Academy of Sciences, 2006Abstract: The inadequate response of immune cells to circulating apoptotic products, such as nucleosomal DNA fragments, is assumed to be a potent stimulus for the production of autoantibodies during the pathogenesis and progression of systemic lupus erythematosus (SLE). Here, we analyzed the levels of circulating nucleosomes, caspases, and C‐reactive
Holdenrieder, Stefan +7 more
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Optimization of Restriction Fragment DNA Mapping
Journal of Computational Biology, 1998Consider a mapping project in which overlap of clonal segments is inferred from complete multiple restriction digests. The fragment sizes of the clones are measured with some error, potentially leading to a map with erroneous links. The number of errors in the map depends on the number and types of enzymes used to characterize the clones.
Andrew F. Siegel +4 more
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Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
2003The Klenow fragment of DNA polymerase I is a proteolytic fragment obtained by the treatment of DNA polymerase I with subtilisin. The fragment still has the polymerase and 3'-5' exonuclease activity, but lacks the 5'-3' exonuclease activity of the holoenzyme (1). Radiolabeling of DNA fragments is most efficient with DNA fragments that contain recessed 3'
W, Gaastra, J, Josephsen
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Sperm DNA Fragmentation and Fertility
Elevated levels of sperm deoxyribonucleic acid (DNA) fragmentation (SDF) have been associated with several adverse reproductive outcomes, including: lower natural and assisted reproductive technology (ART) pregnancy rates, abnormal embryo development, and recurrent pregnancy loss.Jessica, Marinaro, Peter N, Schlegel
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Fragment Assembly of DNA [PDF]
, 2003 Presentation prepared for BIO/CS 471: Algorithms for Bioinformatics. The lecture comprises material from the text Fundamental Concepts of Bioinformatics.
Krane, Dan E., Raymer, Michael L.
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Fragmentation of DNA by sonication.
CSH protocols, 2012INTRODUCTIONDNA fragmentation is often necessary prior to library construction or subcloning for DNA sequencing. This protocol describes a method for DNA fragmentation by sonication. During sonication, DNA samples are subjected to hydrodynamic shearing by exposure to brief periods of sonication. DNA that has been sonicated for excessive periods of time
Joseph, Sambrook, David W, Russell
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The effect of sperm DNA fragmentation on ICSI outcomes depending on oocyte quality
Andrology, 2023Daniela Paes De Almeida Ferreira Braga +2 more
exaly