Results 251 to 260 of about 1,932,999 (290)

Isolation of DNA Fragments for Microinjection

2003
The purification of a DNA fragment for microinjection is extremely important. This chapter describes a rapid and efficient technique for isolating specific DNA fragments from agarose gels run in Tris-acetate buffer, and was first described by Vogelstein and Gillespie.
Smith, D.R., Murphy, D.
openaire   +2 more sources

Measurement of Apoptosis by DNA Fragmentation

2004
Classical apoptotic cell death can be defined by certain morphological and biochemical characteristics that distinguish it from other forms of cell death. One such feature, which is a hallmark of apoptosis, is DNA fragmentation. In dying cells, DNA is cleaved by an endonuclease that fragments the chromatin into nucleosomal units, which are multiples of
Demetrius, Matassov, Terri, Kagan, Julie, Leblanc, Marianna, Sikorska, Zahra, Zakeri   +4 more
openaire   +2 more sources

Nucleosomal DNA Fragments in Autoimmune Diseases

Annals of the New York Academy of Sciences, 2006
Abstract:  The inadequate response of immune cells to circulating apoptotic products, such as nucleosomal DNA fragments, is assumed to be a potent stimulus for the production of autoantibodies during the pathogenesis and progression of systemic lupus erythematosus (SLE). Here, we analyzed the levels of circulating nucleosomes, caspases, and C‐reactive
Holdenrieder, Stefan, Eichhorn, Peter, Beuers, Ulrich, Samtleben, Walter, Schoenermarck, Ulf, Zachoval, Reinhart, Nagel, Dorothea, Stieber, Petra   +7 more
openaire   +3 more sources

Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase

2003
The Klenow fragment of DNA polymerase I is a proteolytic fragment obtained by the treatment of DNA polymerase I with subtilisin. The fragment still has the polymerase and 3'-5' exonuclease activity, but lacks the 5'-3' exonuclease activity of the holoenzyme (1). Radiolabeling of DNA fragments is most efficient with DNA fragments that contain recessed 3'
W, Gaastra, J, Josephsen
openaire   +2 more sources

Circulating tumor DNA in advanced solid tumors: Clinical relevance and future directions

Ca-A Cancer Journal for Clinicians, 2021
Heather Parsons, Atish D. Choudhury, Michael L Cheng   +2 more
exaly  

Sperm DNA fragmentation testing: Summary evidence and clinical practice recommendations

Andrologia, 2021
Sheena Lewis, Peter Humaidan, Sandro C. Esteves   +2 more
exaly  

Sperm DNA Fragmentation and Fertility

Elevated levels of sperm deoxyribonucleic acid (DNA) fragmentation (SDF) have been associated with several adverse reproductive outcomes, including: lower natural and assisted reproductive technology (ART) pregnancy rates, abnormal embryo development, and recurrent pregnancy loss.
Jessica, Marinaro, Peter N, Schlegel
openaire   +2 more sources

FRAGMENTATION OF DNA

2022
APONE LYNNE, SEXTON BRITTANY S, HEIDER MARGARET, WILLIAMS LOUISE JS, DIMALANTA EILEEN T   +4 more
openaire   +5 more sources

The effect of sperm DNA fragmentation on ICSI outcomes depending on oocyte quality

Andrology, 2023
Amanda Souza Setti, Daniela Braga, Rodrigo R Provenza   +2 more
exaly  

The Biology of Cell-free DNA Fragmentation and the Roles of DNASE1, DNASE1L3, and DFFB

American Journal of Human Genetics, 2020
Rossa W K Chiu, Kathy O Lui, Meng Ni
exaly