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DNA fragmentation in spermatozoa: a historical review [PDF]
Andrology, 2017 Sperm DNA Fragmentation has been extensively studied for more than a decade. In the 1940s the uniqueness of the spermatozoa protein complex which stabilizes the DNA was discovered.
Jens Fedder
exaly +6 more sources
DNA fragmentation in apoptosis [PDF]
Cell Research, 2000 Cleavage of chromosomal DNA into oligonucleosomal size fragments is an integral part of apoptosis. Elegant biochemical work identified the DNA fragmentation factor (DFF) as a major apoptotic endonuclease for DNA fragmentation in vitro. Genetic studies in mice support the importance of DFF in DNA fragmentation and possibly in apoptosis in vivo.
Ming Xu, John H. Zhang
exaly +3 more sources
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Seminars in Reproductive Medicine, 2021
AbstractSperm DNA fragmentation refers to the accumulation of adducts as well as single- or double-strand DNA breaks and reflects the sperm DNA quality. Current data suggest that there are differences in sperm DNA quality among individuals with high or low fertility, and this observation has led to the idea that testing sperm DNA fragmentation could be
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AbstractSperm DNA fragmentation refers to the accumulation of adducts as well as single- or double-strand DNA breaks and reflects the sperm DNA quality. Current data suggest that there are differences in sperm DNA quality among individuals with high or low fertility, and this observation has led to the idea that testing sperm DNA fragmentation could be
openaire +2 more sources
Cytochalasin B induces cellular DNA fragmentation
FASEB Journal, 1990 Cellular DNA fragmentation can be induced in many biological instances without plasma membrane damage. The fungal metabolite, cytochalasin B, is capable of modifying numerous cellular functions related to DNA synthesis.
Belkis Landa‐Gonzalez +5 more
exaly +2 more sources
Experimental Cell Research, 2000
Degradation of nuclear DNA into nucleosomal units is one of the hallmarks of apoptotic cell death. It occurs in response to various apoptotic stimuli in a wide variety of cell types. Molecular characterization of this process identified a specific DNase (CAD, caspase-activated DNase) that cleaves chromosomal DNA in a caspase-dependent manner.
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Degradation of nuclear DNA into nucleosomal units is one of the hallmarks of apoptotic cell death. It occurs in response to various apoptotic stimuli in a wide variety of cell types. Molecular characterization of this process identified a specific DNase (CAD, caspase-activated DNase) that cleaves chromosomal DNA in a caspase-dependent manner.
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The role of DNA fragmentation in apoptosis
Trends in Cell Biology, 1995The formation of distinct DNA fragments of oligonucleosomal size (180-200 bp lengths) is a biochemical hallmark of apoptosis in many cells. Recent observations also suggest large DNA fragments and even single-strand cleavage events occur during cell death. These observations have raised many questions. What are the types of DNA cleavage observed during
C D, Bortner +2 more
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Microelectrophoresis for the separation of DNA fragments
ELECTROPHORESIS, 1992AbstractA methodology has been developed which significantly reduces the linear dimension necessary for the electrophoretic separation of DNA fragments and oligonucleotides. DNA fragments are rapidly separated into compact, resolvable microscopic banding patterns which can be detected using a high‐resolution electronic imaging system.
M J, Heller, R H, Tullis
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Quantitative Evaluation of DNA Fragmentation
Bulletin of Experimental Biology and Medicine, 2002A new method for evaluation of DNA fragmentation in tissue is based on computer-aided densitometric standardization of the sum of optical densities of DNA fragments visualized by electrophoresis (180-2500 b. p.) in comparison with the density of high molecular weight DNA in the same sample.
T S, Kalinina, A V, Bannova, N N, Dygalo
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A hypervariable pig DNA fragment
Animal Genetics, 1990Summary A new hypervariable tandem repeat was isolated from the pig genome and characterized by DNA sequence. The use of this DNA fragment as a probe in order to follow allelic segregation and DNA fingerprinting in pigs, horses and rabbits is documented.
W, Coppieters +4 more
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Current Protocols in Molecular Biology, 1991
AbstractThe essence of recombinant DNA technology is the joining of two or more separate segments of DNA to generate a single DNA molecule that is capable of autonomous replication in a given host. The simplest constructions of hybrid DNA molecules involve the cloning of insert sequences into plasmid or bacteriophage cloning vectors.
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AbstractThe essence of recombinant DNA technology is the joining of two or more separate segments of DNA to generate a single DNA molecule that is capable of autonomous replication in a given host. The simplest constructions of hybrid DNA molecules involve the cloning of insert sequences into plasmid or bacteriophage cloning vectors.
openaire +2 more sources