Results 111 to 120 of about 47,217 (278)
A Mechanistic Model of rAAV Production in Synthetic Cell Lines
Biotechnology and Bioengineering, EarlyView.ABSTRACT The recombinant adeno‐associated virus (rAAV) is a widely used vector for gene therapy. Its manufacturing faces significant challenges in producing the large quantities of vectors needed for clinical applications and reducing empty particles.
Prahalad Srinivasan +5 more
wiley +1 more source
Structural visualization of key steps in human transcription initiation. [PDF]
, 2013 Eukaryotic transcription initiation requires the assembly of general transcription factors into a pre-initiation complex that ensures the accurate loading of RNA polymerase II (Pol II) at the transcription start site. The molecular mechanism and function
Fang, Jie +3 more
core +1 more source
BMEMat, EarlyView.Abstract Hepatocellular carcinoma (HCC), ranking as the third leading cause of cancer‐related mortality globally, continues to pose significant therapeutic challenges. Here, we developed an innovative nanosystem, Phl@PT, based on Pt‐TiO2 nanoparticles for the co‐delivery of Phlorezin, presenting a novel approach for HCC treatment through sonodynamic ...
Kairui Liu +13 more
wiley +1 more source
Helicases at Work: The Importance of Nucleic Acids Unwinding Under Cold Stress
DNASeparation of duplex strands of nucleic acids is a vital process in the nucleic acid metabolism and survival of all living organisms. Helicases are defined as enzymes that are intended to unwind the double-stranded nucleic acids.
Theetha L. Pavankumar +3 more
doaj +1 more source
Molecular wrench activity of DNA helicases: Keys to modulation of rapid kinetics in DNA repair. [PDF]
Protein Sci, 2023 Wettasinghe AP +10 more
europepmc +1 more source
The ageing holobiont: crosstalk between telomere dynamics, oxidative stress and the gut microbiome
Biological Reviews, EarlyView.ABSTRACT The gut tissue is at the frontline of early onset of ageing. It exhibits high cell turnover rates and rapid telomere shortening, which can have systemic effects on the developing or senescing organism. We conducted a literature review of studies on the crosstalk between telomere length dynamics, telomerase activity, oxidative stress, and gut ...
Michael L. Pepke +2 more
wiley +1 more source
The conformational changes coupling ATP hydrolysis and translocation in a bacterial DnaB helicase
Nature Communications, 2019 DnaB helicases are motor proteins that couple ATP-hydrolysis to the movement of the protein along single-stranded DNA leading to a separation of double-stranded DNA at the replication fork.
Thomas Wiegand +6 more
doaj +1 more source
eLife, 2017 Replicative helicases are ring-shaped hexamers that encircle DNA for duplex unwinding. The currently accepted view of hexameric helicase function is by steric exclusion, where the helicase encircles one DNA strand and excludes the other, acting as a ...
Lance Langston, Mike O’Donnell
doaj +1 more source
Systemic aging fuels heart failure: Molecular mechanisms and therapeutic avenues
ESC Heart Failure, Volume 12, Issue 2, Page 1059-1080, April 2025.Abstract Systemic aging influences various physiological processes and contributes to structural and functional decline in cardiac tissue. These alterations include an increased incidence of left ventricular hypertrophy, a decline in left ventricular diastolic function, left atrial dilation, atrial fibrillation, myocardial fibrosis and cardiac ...
Zhuyubing Fang +7 more
wiley +1 more source
DNA translocation mechanism of an XPD family helicase
eLife, 2018 The XPD family of helicases, that includes human disease-related FANCJ, DDX11 and RTEL1, are Superfamily two helicases that contain an iron-sulphur cluster domain, translocate on ssDNA in a 5’−3’ direction and play important roles in genome stability ...
Kaiying Cheng, Dale B Wigley
doaj +1 more source