Results 91 to 100 of about 635,921 (300)

Single nucleotide polymorphism discrimination assisted by improved base stacking hybridization using oligonucleotide microarrays

BioTechniques, 2003
Efficiencies of mismatch discrimination using size-varied capture probes were examined at various hybridization temperatures. The probes were 17, 15, 13, 11, 9, and 7 nucleotides long and contained single-base mismatches at their 3′ ends.
D. Wang, H. Gao, R. Zhang, X. Ma, Y. Zhou, J. Cheng   +5 more
doaj   +1 more source

NKCC1: A key regulator of glioblastoma progression

Molecular Oncology, EarlyView.
Glioblastoma (GBM) progression is driven by disrupted chloride cotransporter homeostasis. NKCC1 is highly expressed in stem‐like, astrocytic, and progenitor cells, correlating with earlier recurrence, while overall survival remains unaffected. NKCC1 serves as a prognostic marker and potential therapeutic target, linking chloride transporter imbalance ...
Anja Thomsen, Diana Freitag, Madlen Haase, Christian Senft, Falko Schwarz, Silke Keiner   +5 more
wiley   +1 more source

High-Sensitivity Hybridization Assay for Quantitation of Residual E. coli DNA

BioTechniques, 2002
Impurity assays for recombinant protein therapeutics are essential to ensure batchto-batch consistency and to meet the FDA's criteria for a well-characterized biopharmaceutical.
Xiaoying Ji, Kwan Lee, Byron DiPaolo
doaj   +1 more source

Long-Range Chromosomal Mapping of the Carcinoembryonic Antigen (CEA) Gene Family Cluster [PDF]

, 1992
A long-range physical map of the carcinoembryonic antigen (CEA) gene family cluster, which is located on the long arm of chromosome 19, has been constructed.
Arakawa, Barnett, Barnett, Barnett, Benchimol, Berling, Bird, Bischoff-Delaloye, Bohn, Bordes, Brandriff, Branscomb, Brook, Brook, Brook, Carrano, Carrano, Chomczynski, Church, de Jong, Efstratiadis, Feinberg, Fritsch, Gold, Gold, Hinoda, Hinoda, Holland, Huang, Kamarck, Kodelja, Kuroki, Leusch, Lin, Mach, Maniatis, Nap, Niemann, Oikawa, Paxton, Pignatelli, Ropers, Sambrook, Sanger, Schonk, Schrewe, Schwartz, Sharkey, Shively, Slightom, Stallings, Steinmetz, Streydio, Svenberg, Tatarinov, Tatarinov, Thompson, Thompson, Thompson, Thompson, Thompson, Tonegawa, von Kleist, von Kleist, Willcocks, Willcocks, Willcocks, Zimmermann, Zimmermann, Zimmermann   +69 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Specific staining of human chromosomes in Chinese hamster x man hybrid cell lines demonstrates interphase chromosome territories [PDF]

, 1985
In spite of Carl Rabl's (1885) and Theodor Boveri's (1909) early hypothesis that chromosomes occupy discrete territories or domains within the interphase nucleus, evidence in favor pf this hypothesis has been limited and indirect so far in higher plants ...
AB Murray, C Rabl, C Zorn, DA Agard, DE Comings, DE Comings, DJ DuPraw, E Therman, F Vogel, GA Rappold, GA Rappold, J Hozier, J Sedat, JJ Leary, JS Lebkowski, JT Finch, JW Littlefield, K Grell, K Sperling, L Avivi, L Hens, L Manuelidis, L Manuelidis, LM Kunkel, M Feldman, M Raith, MD Bennett, PR Langer, PR Langer-Safer, R Hancock, S Wischnitzer, SM Stack, T Cremer, T Cremer, Th Boveri   +34 more
core   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Defects Can Increase the Melting Temperature of DNA-Nanoparticle Assemblies

, 2007
DNA-gold nanoparticle assemblies have shown promise as an alternative technology to DNA microarrays for DNA detection and RNA profiling. Understanding the effect of DNA sequences on the melting temperature of the system is central to developing reliable ...
Harris, Nolan C., Kiang, Ching-Hwa
core   +1 more source

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source