Hybridization Parameters Revisited: Solutions Containing SDS
BioTechniques, 2002 Salt concentration governs nucleic acid hybridization according to the Schildkraut-Lifson equation. High concentrations of SDS are used in some common protocols, but the effects of SDS on hybridization stringency have not been reported.
Ken Rose, John O. Mason, Richard Lathe
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Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]
, 1992 Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke +33 more
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Epidemiological Typing of Moraxella Catarrhalis by Pulsed Field Gel Electrophoresis
Canadian Journal of Infectious Diseases, 1995 Pulsed field gel electrophoresis (pfge) was used to compare 59 strains of Moraxella catarrhalis to evaluate pfge for the epidemiological typing of this organism.
Susan M Davison +4 more
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Reusing Nylon Membranes for Radioactive Hybridizations
BioTechniques, 2000 We describe a procedure for recycling nylon hybridization membranes, enabling their repeated use for radioactive Southern hybridization analysis of different DNA samples.
David W. Thornbury, Mark L. Farman
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Studies on Nucleic Acid Reassociation Kinetics: Retarded Rate of Hybridization of RNA with Excess DNA [PDF]
, 1977 The rate of reaction of excess double-stranded bacteriophage phi X174 and plasmid RSF2124 DNA drivers with enzymatically synthesized asymmetric RNA tracers was measured. Other reactions were carried out with excess Escherichia coli DNA and E.
Britten, Roy J. +3 more
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Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84 [PDF]
, 1986 The localization of chromosome 18 in human interphase nuclei is demonstrated by use of radioactive and nonradioactive in situ hybridization techniques with a DNA clone designated L1.84.
A. Brückner +33 more
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Bidirectional natural hybridization between sympatric Ligularia vellerea and L. subspicata
Plant Diversity, 2017 Natural hybridization has been regarded as a crucial pathway of speciation and provides the raw materials for the evolution of biodiversity. The interspecific natural hybridization of the genus Ligularia Cass.
Huai Ning, Jiaojun Yu, Xun Gong
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Phylogeny and DNA-DNA hybridization. [PDF]
Molecular Biology and Evolution, 1986 Resolving the phylogeny of the hominoid primates is a test case for molecular systematic methods because, by most genetic measures, humans, chimpanzees, and gorillas are very closely related. Recently, Sibley and Ahlquist (1984) applied DNADNA hybridization techniques to infer the hominoid phylogenetic tree with humans and chimpanzees closest ...
M, Ruvolo, T F, Smith
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Kindred DNA Amplification from Two Distinct Populations of cDNA Fragments
BioTechniques, 2003 Kindred DNA amplification is a novel and cost-effective method developed to isolate common cDNA fragments between two distinct cDNA populations. Unlike subtractive hybridization, which discards common sequences, kindred DNA amplification isolates and ...
Ulo Puurand, Lumme Kadaja, Enn K. Seppet
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Genome composition of 'Elatior'-begonias hybrids analyzed by genomic in situ hybridisation [PDF]
, 2010 Interspecific hybridization of various tuberous Begonia species hybrids with Begonia socotrana results in so-called 'Elatior'-begonias hybrids (B. x hiemalis Fotsch).
Laak, W.A. +3 more
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