Hybridization Parameters Revisited: Solutions Containing SDS
BioTechniques, 2002 Salt concentration governs nucleic acid hybridization according to the Schildkraut-Lifson equation. High concentrations of SDS are used in some common protocols, but the effects of SDS on hybridization stringency have not been reported.
Ken Rose, John O. Mason, Richard Lathe
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Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe [PDF]
, 1992 A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures.
Eichhorn, Werner +3 more
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Epidemiological Typing of Moraxella Catarrhalis by Pulsed Field Gel Electrophoresis
Canadian Journal of Infectious Diseases, 1995 Pulsed field gel electrophoresis (pfge) was used to compare 59 strains of Moraxella catarrhalis to evaluate pfge for the epidemiological typing of this organism.
Susan M Davison +4 more
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Reusing Nylon Membranes for Radioactive Hybridizations
BioTechniques, 2000 We describe a procedure for recycling nylon hybridization membranes, enabling their repeated use for radioactive Southern hybridization analysis of different DNA samples.
David W. Thornbury, Mark L. Farman
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Furan-PNA : a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA [PDF]
, 2016 We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy.
Corradini, Roberto +3 more
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Phylogeny and DNA-DNA hybridization. [PDF]
Molecular Biology and Evolution, 1986 Resolving the phylogeny of the hominoid primates is a test case for molecular systematic methods because, by most genetic measures, humans, chimpanzees, and gorillas are very closely related. Recently, Sibley and Ahlquist (1984) applied DNADNA hybridization techniques to infer the hominoid phylogenetic tree with humans and chimpanzees closest ...
M, Ruvolo, T F, Smith
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Development of Si-based electrical biosensors: Simulations and first experimental results
Sensing and Bio-Sensing Research, 2015 In this work, we simulated and experimentally assessed the possibility to detect, through electrical transduction, hybridization of DNA molecules on MOS-like devices, having different dielectrics: SiO2, Si3N4 and SiO2/Si3N4/SiO2 (ONO).
Marco Favetta +8 more
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DNA-DNA hybridization study of Bradyrhizobium strains. [PDF]
International Journal of Systematic and Evolutionary Microbiology, 2001 DNA-DNA hybridizations were performed between Bradyrhizobium strains, isolated mainly from Faidherbia albida and Aeschynomene species, as well as Bradyrhizobium reference strains. Results indicated that the genus Bradyrhizobium consists of at least 11 genospecies, I to XI.
Willems, A. +6 more
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BMC Biotechnology, 2008 Background The high binding specificity of short 10 to 30 mer oligonucleotide probes enables single base mismatch (MM) discrimination and thus provides the basis for genotyping and resequencing microarray applications.
Mai Timo +5 more
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Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries [PDF]
, 1988 A method of in situ hybridization for visualizing individual human chromosomes from pter to qter, both in metaphase spreads and interphase nuclei, is reported.
B Trask +39 more
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