Results 51 to 60 of about 635,921 (300)

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Characterization of denaturation and renaturation of DNA for DNA hybridization [PDF]

Environmental Health and Toxicology, 2014
Objectives The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization.
Xiaofang Wang, Hyun Jeong Lim, Ahjeong Son   +2 more
doaj   +1 more source

Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules [PDF]

, 2015
Rhizobial strains isolated from effective root nodules of field-grown lentil (Lens culinaris) from different parts of Bangladesh were previously analysed using sequences of the 16S rRNA gene, three housekeeping genes (recA, atpD and glnll) and three ...
Braun, Markus Santosh, Clercx, Pia, Everall, Isobel, Rashid, M Harun-Or, Willems, Anne, Wink, Michael, Young, J Peter W   +6 more
core   +2 more sources

Structural biology of ferritin nanocages

FEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Quantitative Analysis of Nucleic Acid Hybridization on Magnetic Particles and Quantum Dot-Based Probes

Sensors, 2009
In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-,
Alexey Dan Chin Yu, Sek Mardy, Felix Bestvater, Philippe Buchy, Sun Hee Lim   +4 more
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Molecular detection methods of human papillomavirus (HPV)

The International Journal of Biological Markers, 2009
Human papillomavirus (HPV) testing can identify women at risk of cervical cancer. Currently, molecular detection methods are the gold standard for identification of HPV.
Apostolos Zaravinos, Ioannis N. Mammas, George Sourvinos, Demetrios A. Spandidos   +3 more
doaj   +1 more source

DNA-based Biosensor for Detection of Ganoderma boninense, an Oil Palm Pathogen Utilizing Newly Synthesized Ruthenium Complex [Ru(phen)2(qtpy)]2+ Based on a PEDOT-PSS/Ag Nanoparticles Modified Electrode

International Journal of Electrochemical Science, 2013
An electrochemical DNA biosensor has been developed for detection of ganoderma boninense, an oil palm pathogen utilizing newly synthesized ruthenium [Ru(phen)2(qtpy)]2+ complex as hybridization indicator.
Sabo Wada Dutse, Nor Azah Yusof, Haslina Ahmad, Mohd Zobir Hussein, Zulkarnain Zainal, Roozbeh hushiarian   +5 more
doaj   +1 more source

FRET Imaging of Nonuniformly Distributed DNA SAMs on Gold Reveals the Role Played by the Donor/Acceptor Ratio and the Local Environment in Measuring the Rate of Hybridization

Chemical & Biomedical Imaging, 2023
Mixed DNA SAMs labeled with a fluorophore (either AlexaFluor488 or AlexaFluor647) were prepared on a single crystal gold bead electrode using potential-assisted thiol exchange and studied using Förster resonance energy transfer (FRET).
Adrian Jan Grzędowski, Tianxiao Ma, Dan Bizzotto   +2 more
doaj   +1 more source

A method for nucleic acid hybridization to isolated chromosomes in suspension [PDF]

, 1987
A procedure was developed to provide differential fluorescent staining of metaphase chromosomes in suspension following nucleic acid hybridization. For this purpose metaphase chromosomes were isolated from a Chinese hamster x human hybrid cell line ...
Bier, Frank, Cremer, Christoph, Cremer, Thomas, Dudin, Gertrud, Hausmann, Michael, Schardin, Margit   +5 more
core   +1 more source